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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0502 [new locus tag: SACOL_RS02535 ]
  • pan locus tag?: SAUPAN002174000
  • symbol: SACOL0502
  • pan gene symbol?: mccA
  • synonym:
  • product: cysteine synthase/cystathionine beta-synthase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0502 [new locus tag: SACOL_RS02535 ]
  • symbol: SACOL0502
  • product: cysteine synthase/cystathionine beta-synthase
  • replicon: chromosome
  • strand: +
  • coordinates: 502702..503616
  • length: 915
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGATTACTTATGATTTAATTGGCAATACACCATTAGTACTGTTAGAACATTATAGTGAT
    GATAAAGTTAAAATTTATGCCAAGCTTGAACAATGGAATCCTGGAGGCAGTGTTAAAGAC
    AGACTCGGGAAATATTTAGTAGAGAAGGCAATTCAAGAAGGGCGTGTGCGTGCAGGTCAA
    ACTATTGTTGAAGCGACTGCTGGTAATACAGGCATAGGGTTAGCTATTGCAGCGAATAGA
    CATCATTTGAAATGTAAGATCTTTGCGCCGTATGGTTTTTCAGAAGAAAAGATTAATATT
    ATGATAGCGCTTGGTGCAGAAGTTTCAAGGACGAGTCAGTCTGAAGGTATGCATGGGGCA
    CAATTAGCTGCACGTTCCTATGCTGAAAAATATGGTGCCGTTTATATGAATCAATTTGAA
    TCCGAACATAATCCGGATACATATTTTCATACATTGGGACCCGAATTGACTTCAGCATTA
    CAGCAAATTGATTATTTTGTGGCTGGTATTGGCTCTGGCGGTACATTTACAGGTACCGCA
    CGTTATTTAAAGCAACATCACGTGCAATGTTATGCCGTTGAGCCAGAAGGGTCCGTGTTA
    AATGGAGGGCCAGCTCATGCACATGACACTGAAGGTATCGGTTCTGAGAAATGGCCGATA
    TTTTTAGAGAGACGTCTTGTAGATGGGATATTTACGATTAAAGATCAAGATGCCTTTCGA
    AATGTCAAAAGTTTGGCTATAAATGAAGGGTTGTTAGTAGGCAGTTCTTCAGGTGCAGCA
    TTACAAGGTGCATTGAATTTAAAAGCGCAATTATCTGAAGGTACGATTGTTGTCGTATTT
    CCAGATGGTAGTGATCGATATATGTCTAAGCAAATATTTAATTATGAGGAGAATAATAAT
    GAACAAGAAAACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    915

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0502 [new locus tag: SACOL_RS02535 ]
  • symbol: SACOL0502
  • description: cysteine synthase/cystathionine beta-synthase
  • length: 304
  • theoretical pI: 5.79025
  • theoretical MW: 33261.1
  • GRAVY: -0.3125

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Amino acid biosynthesis Serine family cysteine synthase (TIGR01136; EC 2.5.1.47; HMM-score: 271.8)
    Metabolism Amino acid biosynthesis Serine family cysteine synthase A (TIGR01139; EC 2.5.1.47; HMM-score: 261.7)
    Metabolism Amino acid biosynthesis Serine family cystathionine beta-synthase (TIGR01137; EC 4.2.1.22; HMM-score: 258.4)
    Metabolism Amino acid biosynthesis Serine family cysteine synthase B (TIGR01138; EC 2.5.1.47; HMM-score: 218)
    and 12 more
    Cellular processes Cellular processes Biosynthesis of natural products 2,3-diaminopropionate biosynthesis protein SbnA (TIGR03945; HMM-score: 203.3)
    Metabolism Amino acid biosynthesis Pyruvate family threonine ammonia-lyase (TIGR01127; EC 4.3.1.19; HMM-score: 65.1)
    Metabolism Amino acid biosynthesis Pyruvate family threonine dehydratase (TIGR02079; EC 4.3.1.19; HMM-score: 46.5)
    Metabolism Amino acid biosynthesis Pyruvate family threonine ammonia-lyase, biosynthetic (TIGR01124; EC 4.3.1.19; HMM-score: 42.1)
    Metabolism Amino acid biosynthesis Aspartate family threonine synthase (TIGR00260; EC 4.2.3.1; HMM-score: 39.6)
    Metabolism Amino acid biosynthesis Aromatic amino acid family tryptophan synthase, beta subunit (TIGR00263; EC 4.2.1.20; HMM-score: 35.8)
    ectoine utilization protein EutB (TIGR02991; HMM-score: 30.2)
    Unknown function Enzymes of unknown specificity pyridoxal-phosphate dependent TrpB-like enzyme (TIGR01415; HMM-score: 23.1)
    diaminopropionate ammonia-lyase (TIGR03528; EC 4.3.1.15; HMM-score: 22.4)
    Metabolism Energy metabolism Other diaminopropionate ammonia-lyase family (TIGR01747; EC 4.3.1.15; HMM-score: 19.3)
    Metabolism Energy metabolism Amino acids and amines D-serine ammonia-lyase (TIGR02035; EC 4.3.1.18; HMM-score: 15.8)
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis beta-hydroxyacyl-(acyl-carrier-protein) dehydratase FabZ (TIGR01750; EC 4.2.1.-; HMM-score: 12)
  • TheSEED  :
    • Cystathionine beta-synthase (EC 4.2.1.22)
    Amino Acids and Derivatives Alanine, serine, and glycine Glycine and Serine Utilization  Cystathionine beta-synthase (EC 4.2.1.22)
    and 3 more
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Cysteine Biosynthesis  Cystathionine beta-synthase (EC 4.2.1.22)
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Methionine Biosynthesis  Cystathionine beta-synthase (EC 4.2.1.22)
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Methionine Degradation  Cystathionine beta-synthase (EC 4.2.1.22)
  • PFAM:
    no clan defined PALP; Pyridoxal-phosphate dependent enzyme (PF00291; HMM-score: 209.7)
    and 2 more
    Acyltransferase (CL0228) DUF374; Domain of unknown function (DUF374) (PF04028; HMM-score: 15.2)
    NADP_Rossmann (CL0063) Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 11.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00521
    • TAT(Tat/SPI): 0.000209
    • LIPO(Sec/SPII): 0.000556
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MITYDLIGNTPLVLLEHYSDDKVKIYAKLEQWNPGGSVKDRLGKYLVEKAIQEGRVRAGQTIVEATAGNTGIGLAIAANRHHLKCKIFAPYGFSEEKINIMIALGAEVSRTSQSEGMHGAQLAARSYAEKYGAVYMNQFESEHNPDTYFHTLGPELTSALQQIDYFVAGIGSGGTFTGTARYLKQHHVQCYAVEPEGSVLNGGPAHAHDTEGIGSEKWPIFLERRLVDGIFTIKDQDAFRNVKSLAINEGLLVGSSSGAALQGALNLKAQLSEGTIVVVFPDGSDRYMSKQIFNYEENNNEQEN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:
    SACOL1307hypothetical protein  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CymR* (repression) regulon
    CymR*(TF)important in Cysteine metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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