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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000392
  • pan locus tag?: SAUPAN002174000
  • symbol: JSNZ_000392
  • pan gene symbol?: mccA
  • synonym:
  • product: PLP-dependent cysteine synthase family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000392
  • symbol: JSNZ_000392
  • product: PLP-dependent cysteine synthase family protein
  • replicon: chromosome
  • strand: +
  • coordinates: 421507..422412
  • length: 906
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGATTACTTATGATTTAATTGGCAATACACCATTAGTACTGTTAGAACATTATAGTGAT
    GATAAAGTTAAAATTTATGCCAAGCTTGAACAATGGAATCCTGGAGGCAGTGTTAAAGAC
    AGACTCGGGAAATATTTAGTAGAGAAGGCAATTCAAGAAGGGCGTGTGCGTGCAGGTCAA
    ACTATTGTTGAAGCGACTGCTGGTAATACAGACATAGGGTTAGCTATTGCAGCGAATAGA
    CATCATTTAAAATGTAAGATCTTTGCGCCGTATGGTTTTTCAGAAGAAAAGATTAATATT
    ATGATAGCGCTTGGTGCAGATGTTTCAAGGACGAGTCAGTCTGAAGGTATGCATGGGGCA
    CAATTAGCTGCACGTTCCTATGCTGAAAAATATGGTGCCGTTTATATGAATCAATTTGAA
    TCCGAACATAATCCGGATACATATTTTCATACATTGGGACCCGAATTGACTTCAGCATTA
    CAGCAAATTGATTATTTTGTGGCTGGTATTGGCTCTGGCGGTACATTTACAGGTACCGCA
    CGTTATTTAAAGCAACATCACGTGCAATGTTATGCCGTTGAGCCAGAAGGGTCCGTGTTA
    AATGGAGGGCCAGCTCATGCACATGACACTGAAGGTATCGGTTCTGAGAAATGGCCGATA
    TTTTTAGAGAGACGTCTTGTAGATGGGATATTTACGATTAAAGATCAAGATGCCTTTCGA
    AATGTCAAAAGTTTGGCTATAAATGAAGGGTTGTTAGTAGGCAGTTCTTCAGGTGCAGCA
    TTACAAGGTGCATTGAATTTAAAAGCGCAATTATCTGAAGGTACGATTGTTGTCGTATTT
    CCAGATGGTAGCGATCGATATATGTCTAAGCAAATATTTAATTATGAGGAGAATGATTAT
    GAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    906

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000392
  • symbol: JSNZ_000392
  • description: PLP-dependent cysteine synthase family protein
  • length: 301
  • theoretical pI: 5.56783
  • theoretical MW: 32983.8
  • GRAVY: -0.283721

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Amino acid biosynthesis Serine family cysteine synthase (TIGR01136; EC 2.5.1.47; HMM-score: 266.2)
    Metabolism Amino acid biosynthesis Serine family cysteine synthase A (TIGR01139; EC 2.5.1.47; HMM-score: 256.3)
    Metabolism Amino acid biosynthesis Serine family cystathionine beta-synthase (TIGR01137; EC 4.2.1.22; HMM-score: 252.1)
    Metabolism Amino acid biosynthesis Serine family cysteine synthase B (TIGR01138; EC 2.5.1.47; HMM-score: 213.4)
    and 12 more
    Cellular processes Cellular processes Biosynthesis of natural products 2,3-diaminopropionate biosynthesis protein SbnA (TIGR03945; HMM-score: 199.3)
    Metabolism Amino acid biosynthesis Pyruvate family threonine ammonia-lyase (TIGR01127; EC 4.3.1.19; HMM-score: 63.3)
    Metabolism Amino acid biosynthesis Pyruvate family threonine dehydratase (TIGR02079; EC 4.3.1.19; HMM-score: 45.4)
    Metabolism Amino acid biosynthesis Pyruvate family threonine ammonia-lyase, biosynthetic (TIGR01124; EC 4.3.1.19; HMM-score: 40.7)
    Metabolism Amino acid biosynthesis Aspartate family threonine synthase (TIGR00260; EC 4.2.3.1; HMM-score: 35.2)
    Metabolism Amino acid biosynthesis Aromatic amino acid family tryptophan synthase, beta subunit (TIGR00263; EC 4.2.1.20; HMM-score: 32)
    ectoine utilization protein EutB (TIGR02991; HMM-score: 25.7)
    Unknown function Enzymes of unknown specificity pyridoxal-phosphate dependent TrpB-like enzyme (TIGR01415; HMM-score: 18.4)
    diaminopropionate ammonia-lyase (TIGR03528; EC 4.3.1.15; HMM-score: 18)
    Metabolism Energy metabolism Amino acids and amines D-serine ammonia-lyase (TIGR02035; EC 4.3.1.18; HMM-score: 15.8)
    Metabolism Energy metabolism Other diaminopropionate ammonia-lyase family (TIGR01747; EC 4.3.1.15; HMM-score: 14.7)
    Metabolism Central intermediary metabolism Other 1-aminocyclopropane-1-carboxylate deaminase (TIGR01274; EC 3.5.99.7; HMM-score: 11.9)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    NADP_Rossmann (CL0063) PALP; Pyridoxal-phosphate dependent enzyme (PF00291; HMM-score: 218.4)
    and 2 more
    Acyltransferase (CL0228) DUF374; Domain of unknown function (DUF374) (PF04028; HMM-score: 13.2)
    vWA-like (CL0128) VWA_CoxE; VWA domain containing CoxE-like protein (PF05762; HMM-score: 12.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9975
    • Cytoplasmic Membrane Score: 0.0004
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.002
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00521
    • TAT(Tat/SPI): 0.000209
    • LIPO(Sec/SPII): 0.000556
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MITYDLIGNTPLVLLEHYSDDKVKIYAKLEQWNPGGSVKDRLGKYLVEKAIQEGRVRAGQTIVEATAGNTDIGLAIAANRHHLKCKIFAPYGFSEEKINIMIALGADVSRTSQSEGMHGAQLAARSYAEKYGAVYMNQFESEHNPDTYFHTLGPELTSALQQIDYFVAGIGSGGTFTGTARYLKQHHVQCYAVEPEGSVLNGGPAHAHDTEGIGSEKWPIFLERRLVDGIFTIKDQDAFRNVKSLAINEGLLVGSSSGAALQGALNLKAQLSEGTIVVVFPDGSDRYMSKQIFNYEENDYE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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