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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0482 [new locus tag: SACOL_RS02440 ]
  • pan locus tag?: SAUPAN002139000
  • symbol: SACOL0482
  • pan gene symbol?: lpl5
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0482 [new locus tag: SACOL_RS02440 ]
  • symbol: SACOL0482
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 483981..484772
  • length: 792
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGAATATCTAAAAAGGCTTGCATTGTTAATAAGTGTTATTATTTTGACCATTTTTATA
    ATGGGTTGTGATAGTCAAAGCGATACTGCAGAAAATCCAAAAGAAGGTTCAAAAGAAGCA
    CAAATTAAAAAGAGTTTTTCGAAAACGTTAGATATGTATCCAATTAAGAATCTCGAGGAT
    TTTTATGACAAAGAAGGATATCGAGATGGCGAATTTAAAAAAGATGATAAAGGTACTTGG
    CTAATTAGATCCGAAATAGTTAAACAGCCAAAGGGCAAAGTGATGAAAACAAGAGGTATG
    CAATTATATATTAATAGAAATACCGAAACAGCCAAAGGTTTCTTTGTTTTGAAAGAAATA
    AGTGAAAATAATAATCGTGTAAATAAAGATAAGGAGGAAAAATACGAAGTGAAAATGGTA
    GGAAATAAAATTATTCCTACTGAACAAATTAATGACGAGAAAATAAAAAAAGAAATTGAA
    AACTTCAAGTTTTTTGTGCAATATGGAAACTTTAAAAATTTCGAAAAATACAACAATGGT
    GAGTTTTCATATAATCCTGAAGCACCAATTTATTCGGCTAAATATCAATTACACAACGAT
    GATTACAATGTAAGGCAACTACGTAAAAGATATGACATTTCAACAAAAGAAACACCGAAG
    TTACTTTTGAAAGGTGGAGGAGATTTAAAAAATTCCTCAGTTGGTCAAAACGATATTGAA
    TTTACTTTTGTTGAAAGAAAAGGTGAGAATATTTATTTTAACGATAGTGTTGAATTCATA
    CCAAGTAAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    792

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0482 [new locus tag: SACOL_RS02440 ]
  • symbol: SACOL0482
  • description: hypothetical protein
  • length: 263
  • theoretical pI: 9.1857
  • theoretical MW: 30819.8
  • GRAVY: -0.862738

⊟Function[edit | edit source]

  • TIGRFAM:
    Staphylococcus tandem lipoproteins (TIGR01742; HMM-score: 343.3)
  • TheSEED  :
    • FIG01107840: hypothetical protein
  • PFAM:
    LolA_LolB (CL0048) DUF576; Csa1 family (PF04507; HMM-score: 383.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 4.6
    • Cellwall Score: 2.48
    • Extracellular Score: 2.92
    • Internal Helix: 1
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular possibility: 0
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: IFIMGCD
  • SignalP: Signal peptide LIPO(Sec/SPII) length 22 aa
    • SP(Sec/SPI): 0.000775
    • TAT(Tat/SPI): 0.000047
    • LIPO(Sec/SPII): 0.999013
    • Cleavage Site: CS pos: 22-23. IMG-CD. Pr: 0.9994
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MEYLKRLALLISVIILTIFIMGCDSQSDTAENPKEGSKEAQIKKSFSKTLDMYPIKNLEDFYDKEGYRDGEFKKDDKGTWLIRSEIVKQPKGKVMKTRGMQLYINRNTETAKGFFVLKEISENNNRVNKDKEEKYEVKMVGNKIIPTEQINDEKIKKEIENFKFFVQYGNFKNFEKYNNGEFSYNPEAPIYSAKYQLHNDDYNVRQLRKRYDISTKETPKLLLKGGGDLKNSSVGQNDIEFTFVERKGENIYFNDSVEFIPSK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Lipoprotein [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • data available for N315

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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