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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0455 [new locus tag: SACOL_RS02305 ]
  • pan locus tag?: SAUPAN001990000
  • symbol: SACOL0455
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0455 [new locus tag: SACOL_RS02305 ]
  • symbol: SACOL0455
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 457594..458550
  • length: 957
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGTTAACTAAAGAATTTGCACAACGCGTAGAACTAAGTGAGAAGCAAGTACGTAAAATT
    GTTCAACATTTAGAAGAACGTGGTTACCAACTAAGCAAGACTGAATATCGTGGTCGTGAA
    GCAACTGATTTCAAAGAAGAAGATATCGAGCTTTTCAAAGACATTGCCGACAAAGTAAAA
    CAAACAAATAGTTATGATCTAGCGTTTGATGAATTAGAAAAAGAAAAAGACTTCCTGCAA
    GTCATTGTCAAAAACGATGACAAAAACTTACCTACTAATCAAAATGTCGCTCAACTAGTA
    GAAGATTTACGCCTAGAAATCCAGAAAATGCGCGAAGAACGTCACCTACTTGGTCAAATG
    ATGAATCAAGTACATCAGCAACAACAAGAATTAAAAGAACTTCAAAATCAACTTACATCT
    AAAATCGATTCAAATAGCGAATCCTTAAAAGCCATCCAAACATCACAAGAGGCTATCCAA
    GAAGCGCAAGCCTCTCAAGCAAAAGCATTAGCTGAATCAACAAATAAAGTTGAAAAGAAT
    GCTGTTACTGAAGATAAAGCTGACAGTAAAGATTCGAAGGTTGCTGGAGTGAATACATCA
    ACAGACGCTAAGACGGATACTAAAGCAGAAAATGCAGGCGATGGCACTGCTACTAAAGTA
    GATAAAGAGGATCAAATTTCTGCAACTGAAGCAATTGAAAAAGCCAGCGTTGAGCAGTCT
    AAAAATGACAATGCAGCTGAGACTTCAAATAAAGAAGCTACAGTAGATGCAGAAGCGCAA
    CATGATGCTGAACAACAAGTAGCAGAAGCCCATGCAGAAGCTTCTAAACAAGCTACTTCG
    AACGATTCATTGGAAGCGAAAGCAGAAAATGACAGCACAGCTTCACAGTCAGAAATGTCA
    GAACCAAAACCTCAAGAAGAGAAAAAAGGTTTCTTCGCACGTTTATTCAACCTGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    957

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0455 [new locus tag: SACOL_RS02305 ]
  • symbol: SACOL0455
  • description: hypothetical protein
  • length: 318
  • theoretical pI: 4.46066
  • theoretical MW: 35611.7
  • GRAVY: -1.05126

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA excision repair protein (rad2) (TIGR00600; HMM-score: 3.8)
    Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02168; HMM-score: 3.5)
    Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02168; HMM-score: 3.5)
    Metabolism Transport and binding proteins Cations and iron carrying compounds K+-dependent Na+/Ca+ exchanger (TIGR00927; HMM-score: 3.3)
  • TheSEED  :
    • FIG01108085: hypothetical protein
  • ⊞PFAM:
    no clan defined Med9; RNA polymerase II transcription mediator complex subunit 9 (PF07544; HMM-score: 18.5)
    and 4 more
    HTH (CL0123) HTH_36; Helix-turn-helix domain (PF13730; HMM-score: 14.3)
    NirdL-like_HTH; Siroheme decarboxylase NirDL-like HTH domain (PF22451; HMM-score: 12.2)
    FAD-oxidase_C (CL0277) FAD_SOX; Flavin adenine dinucleotide (FAD)-dependent sulfhydryl oxidase (PF18371; HMM-score: 10.5)
    HTH (CL0123) HTH_54; ParA helix turn helix domain (PF18607; HMM-score: 9.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.8369
    • Cytoplasmic Membrane Score: 0.0327
    • Cell wall & surface Score: 0.1159
    • Extracellular Score: 0.0145
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003324
    • TAT(Tat/SPI): 0.000347
    • LIPO(Sec/SPII): 0.000788
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLTKEFAQRVELSEKQVRKIVQHLEERGYQLSKTEYRGREATDFKEEDIELFKDIADKVKQTNSYDLAFDELEKEKDFLQVIVKNDDKNLPTNQNVAQLVEDLRLEIQKMREERHLLGQMMNQVHQQQQELKELQNQLTSKIDSNSESLKAIQTSQEAIQEAQASQAKALAESTNKVEKNAVTEDKADSKDSKVAGVNTSTDAKTDTKAENAGDGTATKVDKEDQISATEAIEKASVEQSKNDNAAETSNKEATVDAEAQHDAEQQVAEAHAEASKQATSNDSLEAKAENDSTASQSEMSEPKPQEEKKGFFARLFNL

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3] [4]
  • quantitative data / protein copy number per cell: 1088 [5]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: 10.83 h [6]

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  5. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  6. ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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