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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL0334 [new locus tag: SACOL_RS01680 ]
- pan locus tag?: SAUPAN001434000
- symbol: SACOL0334
- pan gene symbol?: —
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL0334 [new locus tag: SACOL_RS01680 ]
- symbol: SACOL0334
- product: hypothetical protein
- replicon: chromosome
- strand: +
- coordinates: 361971..362132
- length: 162
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3236896 NCBI
- RefSeq: YP_185226 NCBI
- BioCyc: see SACOL_RS01680
- MicrobesOnline: 911805 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
61
121ATGAGCGACACATATAAAAGCTACCTAGTAGCAGTACTATGCTTCACAGTCTTAGCAATT
GTACTTATGCCGTTTCTATACTTCACTACAGCATGGTCAATTGCGGGATTCGCAAGTATC
GCAACATTCATATTTTATAAAGAATACTTTTATGAAGAATAA60
120
162
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL0334 [new locus tag: SACOL_RS01680 ]
- symbol: SACOL0334
- description: hypothetical protein
- length: 53
- theoretical pI: 4.1475
- theoretical MW: 6187.23
- GRAVY: 0.967925
⊟Function[edit | edit source]
- TIGRFAM:
- TheSEED :
- Phage phi 11 orf10a protein homolog
- PFAM: no clan defined DUF1270; Protein of unknown function (DUF1270) (PF06900; HMM-score: 131.9)and 2 moreEcsB; Bacterial ABC transporter protein EcsB (PF05975; HMM-score: 14.4)DUF2244; Integral membrane protein (DUF2244) (PF10003; HMM-score: 13.3)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0.32
- Cytoplasmic Membrane Score: 9.55
- Cellwall Score: 0.12
- Extracellular Score: 0.01
- Internal Helix: 1
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: 0.5
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.027745
- TAT(Tat/SPI): 0.007712
- LIPO(Sec/SPII): 0.051244
- predicted transmembrane helices (TMHMM): 1
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MSDTYKSYLVAVLCFTVLAIVLMPFLYFTTAWSIAGFASIATFIFYKEYFYEE
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: no data available
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
SACOL0334 is identical to the eleventh gene product of prophage ΦNM1 in Staphylococcus aureus Newman. Gp11 is a metabolic inhibitor that binds to host MurG and DivIC and impairs Lipid II synthesis and, ultimately, cell division.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
⊟Relevant publications[edit | edit source]
- ↑ Qi Xu, Li Tang, Weilin Liu, Neng Xu, Yangbo Hu, Yong Zhang, Shiyun Chen
Phage protein Gp11 blocks Staphylococcus aureus cell division by inhibiting peptidoglycan biosynthesis.
mBio: 2024, 15(6);e0067924
[PubMed:38752726] [WorldCat.org] [DOI] (I p)