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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0214 [new locus tag: SACOL_RS01095 ]
  • pan locus tag?: SAUPAN001095000
  • symbol: SACOL0214
  • pan gene symbol?: fadE
  • synonym:
  • product: long-chain-fatty-acid--CoA ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0214 [new locus tag: SACOL_RS01095 ]
  • symbol: SACOL0214
  • product: long-chain-fatty-acid--CoA ligase
  • replicon: chromosome
  • strand: -
  • coordinates: 253142..254647
  • length: 1506
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAATTTCGATTGGATAAAAACGCGTTCAGACTTCGATGATGACAAGCCTGCCGTTATT
    GATCACGCAAAACAAACATCTTGGACATACCAACAACTCAATGCACGCGCTGATAATATG
    GCACATTATTTAACATCTCAAGGTGTTAAAAAAGGCGATGTTATCGGTATTTTTGCGCCA
    AATGATATTGCAATATTAGATTTATTGTTTGCTTGTTTTAAAACAGGTGCGGTTTTTTTA
    CCATTGAATTGGCGGCTTAACCCAAAAGAAATTGCAGCCATTGTGGAAGATGCACAATTA
    AAACTGCTCTTCTATGCTGAAAAACATTTAAGTTCACTCACCGATATTGACCAAAACTTA
    TTGCATATGGATATTGATGTGGCGCAATATGATGAAATCGTAAATCCAGATTATCACCAA
    CCTTTTCAAGCAACACCTGTTGAACCACAAGATCTTGCAGCATTAATTTATACAAGTGGT
    ACAACCGGATCACCTAAAGGTGTGATGTTCTCATATGAATCATTTGTTCATAACGGTGCA
    AACTTAGAGCTGACGTATAAGTTCAATTCAAACTATATTACGATTGTATCAACACCAATG
    TTCCATGTTTTAGGGTTTAACGATACTGTATTACCAGTATTAATGTCAGGTGGCACACTT
    ATTCTTCAACGTTACTTTAACGGCGAAGAATTGAATGACATGATTGCACAATATCACCCT
    ACATTTATTATCATGATTCCGACAATGTATTATAGTACGCTACGTGCTAGTAATTTTAAT
    CCTGAAAATTTTAGAGCTATGGATTATATCATCCAAGGTGGTTCACAACCATTACCAAGT
    ATCCAAGCAGCTTTTAAACAATATGGCATAAACATTATTAACGGCTATGGTTTAACTGAA
    GCACCTCTTGTACTTGTTAATACACCGGAAAATTCAAAACGTAAGCCAATGAGTATTGGT
    AAAGCAGTCATGTTCGTTGATGCACGTATCCTTGATGATAACGGTGAGGAAGTACCTACT
    GGTGAGATTGGCGAACTTGCAATTAAGGCTAAAAATGTCACGCCAGGATATTGGAATAAA
    CCAGCAGAGACTGCCAAAGCATTTCATGGTCGATATTTATTAACTGGTGACTTAGCGAAG
    ATGGACAACGATGGCGATATATTTATTATTGACCGCAAAAAAGAATTAATCATAACTGGT
    GGCGAAAATGTCTTACCATCCGAAGTCGAAAATGCTTTAGCTGAGCATCCACTAGTAGAC
    CGGTGTGTGGTCGTTGGCTATGATCATCCAAAATATGGTGAATCAATTGCTGCAGCCATT
    ATACTTCGCGAAGATGAACCTCATTACGCTGAAATTTTAAATCAACATATGCGAAGTCGT
    TTAGCAGGTTATAAAGTCCCAAGAATGTATGTACCAGTGACACATATGCCGTTAAACAGT
    ACGCAGAAACCAGATAAACTTGCGATTCGACAAATGATGAATGACAAAGTCTCGCAAACA
    CTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1506

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0214 [new locus tag: SACOL_RS01095 ]
  • symbol: SACOL0214
  • description: long-chain-fatty-acid--CoA ligase
  • length: 501
  • theoretical pI: 5.18811
  • theoretical MW: 56204.8
  • GRAVY: -0.198802

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone O-succinylbenzoate-CoA ligase (TIGR01923; EC 6.2.1.26; HMM-score: 271.9)
    acyl-CoA ligase (AMP-forming), exosortase A-associated (TIGR03098; HMM-score: 249.5)
    amino acid adenylation domain (TIGR01733; HMM-score: 227.1)
    and 11 more
    cyclohexanecarboxylate-CoA ligase (TIGR03208; EC 6.2.1.-; HMM-score: 193.3)
    dicarboxylate--CoA ligase PimA (TIGR03205; EC 6.2.1.23; HMM-score: 181.4)
    benzoate-CoA ligase family (TIGR02262; HMM-score: 164.3)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan D-alanine--poly(phosphoribitol) ligase, subunit 1 (TIGR01734; EC 6.1.1.13; HMM-score: 152.5)
    Metabolism Transport and binding proteins Cations and iron carrying compounds (2,3-dihydroxybenzoyl)adenylate synthase (TIGR02275; EC 2.7.7.58; HMM-score: 144.2)
    acetate--CoA ligase (TIGR02188; EC 6.2.1.1; HMM-score: 123.1)
    propionate--CoA ligase (TIGR02316; EC 6.2.1.17; HMM-score: 68.9)
    L-aminoadipate-semialdehyde dehydrogenase (TIGR03443; EC 1.2.1.31; HMM-score: 53.7)
    Metabolism Central intermediary metabolism Other acetoacetate-CoA ligase (TIGR01217; EC 6.2.1.16; HMM-score: 35.4)
    4-coumarate--CoA ligase (TIGR02372; EC 6.2.1.12; HMM-score: 27.3)
    Metabolism Energy metabolism Other phenylacetate-CoA ligase (TIGR02155; EC 6.2.1.30; HMM-score: 11.8)
  • TheSEED  :
    • Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Biotin Biotin biosynthesis  Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3)
  • PFAM:
    ANL (CL0378) AMP-binding; AMP-binding enzyme (PF00501; HMM-score: 330.5)
    and 1 more
    AMP-binding_C (CL0531) AMP-binding_C; AMP-binding enzyme C-terminal domain (PF13193; HMM-score: 53.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.009649
    • TAT(Tat/SPI): 0.00044
    • LIPO(Sec/SPII): 0.001227
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNFDWIKTRSDFDDDKPAVIDHAKQTSWTYQQLNARADNMAHYLTSQGVKKGDVIGIFAPNDIAILDLLFACFKTGAVFLPLNWRLNPKEIAAIVEDAQLKLLFYAEKHLSSLTDIDQNLLHMDIDVAQYDEIVNPDYHQPFQATPVEPQDLAALIYTSGTTGSPKGVMFSYESFVHNGANLELTYKFNSNYITIVSTPMFHVLGFNDTVLPVLMSGGTLILQRYFNGEELNDMIAQYHPTFIIMIPTMYYSTLRASNFNPENFRAMDYIIQGGSQPLPSIQAAFKQYGINIINGYGLTEAPLVLVNTPENSKRKPMSIGKAVMFVDARILDDNGEEVPTGEIGELAIKAKNVTPGYWNKPAETAKAFHGRYLLTGDLAKMDNDGDIFIIDRKKELIITGGENVLPSEVENALAEHPLVDRCVVVGYDHPKYGESIAAAIILREDEPHYAEILNQHMRSRLAGYKVPRMYVPVTHMPLNSTQKPDKLAIRQMMNDKVSQTL

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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