PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA2356 [new locus tag: SA_RS13495 ]
• pan locus tag^?: SAUPAN006235000
• symbol: isaA
• pan gene symbol^?: isaA
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA2356 [new locus tag: SA_RS13495 ]
• symbol: isaA
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 2648126..2648827
• length: 702
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1125284 NCBI
• RefSeq: NP_375681 NCBI
• BioCyc: see SA_RS13495
• MicrobesOnline: 104707 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAAAAAGACAATTATGGCATCATCATTAGCAGTGGCATTAGGTGTAACAGGTTACGCA
  GCAGGTACAGGACATCAAGCACACGCTGCTGAAGTAAACGTTGATCAAGCACACTTAGTT
  GACTTAGCGCATAATCACCAAGATCAATTAAATGCAGCTCCAATCAAAGATGGTGCATAT
  GACATCCACTTTGTAAAAGATGGTTTCCAATATAACTTTACTTCAAATGGTACTACATGG
  TCATGGAGCTATGAAGCAGCTAATGGTCAAACTGCTGGTTTCTCAAACGTTGCAGGTGCA
  GACTACACTACTTCATACAACCAAGGTTCAGATGTACAATCAGTAAGCTACAATGCACAA
  TCAAGTAACTCAAACGTTGAAGCTGTTTCAGCTCCAACTTACCATAACTACAGCACTTCA
  ACTACTTCAAGTTCAGTGAGATTAAGCAATGGTAATACTGCAGGTGCTACTGGTTCATCA
  GCAGCTCAAATCATGGCTCAACGTACTGGTGTTTCAGCTTCTACATGGGCTGCAATCATC
  GCTCGTGAATCAAATGGTCAAGTAAATGCTTACAACCCATCAGGTGCTTCAGGTTTATTC
  CAAACTATGCCAGGTTGGGGTCCGACAAACACTGTTGACCAACAAATCAACGCAGCTGTT
  AAAGCATACAAAGCACAAGGTTTAGGTGCTTGGGGATTCTAA
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  702
  

⊟Protein

⊟General

• locus tag: SA2356 [new locus tag: SA_RS13495 ]
• symbol: IsaA
• description: hypothetical protein
• length: 233
• theoretical pI: 6.38594
• theoretical MW: 24204
• GRAVY: -0.338197

⊟Function

• reaction:
  EC 3.2.-.-?  ExPASy
• TIGRFAM:
• TheSEED  :
  • Immunodominant staphylococcal antigen A precursor
• PFAM:
  Lysozyme (CL0037) SLT; Transglycosylase SLT domain (PF01464; HMM-score: 28.3)
  and 2 more
  Transglycosylas; Transglycosylase-like domain (PF06737; HMM-score: 21.9)
  no clan defined DUF945; Bacterial protein of unknown function (DUF945) (PF06097; HMM-score: 15.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.02
  • Extracellular Score: 9.98
  • Internal Helices: 0
• LocateP: N-terminally anchored (with CS)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: -0.17
  • Signal peptide possibility: 1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: GTGHQAHA
• SignalP: Signal peptide SP(Sec/SPI) length 29 aa
  • SP(Sec/SPI): 0.994875
  • TAT(Tat/SPI): 0.002931
  • LIPO(Sec/SPII): 0.001546
  • Cleavage Site: CS pos: 29-30. AHA-AE. Pr: 0.9855
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15928148 NCBI
• RefSeq: NP_375681 NCBI
• UniProt: P99160 UniProt

⊟Protein sequence

• MKKTIMASSLAVALGVTGYAAGTGHQAHAAEVNVDQAHLVDLAHNHQDQLNAAPIKDGAYDIHFVKDGFQYNFTSNGTTWSWSYEAANGQTAGFSNVAGADYTTSYNQGSDVQSVSYNAQSSNSNVEAVSAPTYHNYSTSTTSSSVRLSNGNTAGATGSSAAQIMAQRTGVSASTWAAIIARESNGQVNAYNPSGASGLFQTMPGWGPTNTVDQQINAAVKAYKAQGLGAWGF

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• data available for NCTC8325

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J Microbiol Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)