PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA2080 [new locus tag: SA_RS11955 ]
• pan locus tag^?: SAUPAN005749000
• symbol: SA2080
• pan gene symbol^?: ydbM
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA2080 [new locus tag: SA_RS11955 ]
• symbol: SA2080
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 2340324..2341478
• length: 1155
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1125003 NCBI
• RefSeq: NP_375398 NCBI
• BioCyc: see SA_RS11955
• MicrobesOnline: 104424 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  
  ATGAATGATATGGATAATTCCTTTTTAATAACAACGGAAATTCAAAGAAAATGGATTGAA
  AAATTCAAAGTAATTAGAGATACATTTAAGGCTAAAGCTGAATATAATGATCAACATAGC
  CAATTTCCATATAAAAATATTGAATGGTTAATTAAAGAAGGTTATGGAAAATTAACGTTA
  CCAAAAGCATATGGTGGTGAAGGTGCGACCATAGAAGACATGGTTATTTTGCAATCATTT
  TTAGGCGAACTTGATGGTGCCACAGCATTATCTATTGGTTGGCATGTGAGTGTCGTAGGA
  CAAATTTATGAACAGAAATTATGGTCTCAAGATATGTTGGAGCAATTTGCTGTTGAAATT
  AATAATGGTGCATTAGTTAATAGAGCAGTTAGTGAAGCTGAAATGGGTAGTCCAACAAGA
  GGGGGAAGACCAAGTACACATGCTGTTAAAGCTGATGATGGGTATATTTTAAATGGTGTG
  AAGACATATACATCAATGAGTAAAGCACTAACACATATTATTGTTGCTGCTTATATAGAA
  GAATTAGAAAGTGTTGGTTTTTTCTTAGTAGACAGGAATTTGCCTGGTGTTGAAATTGCC
  GATAATTGGGACGTATTAGGTATGCGAGCGACAGAAAGTCATGATTTAATATTAAATGAT
  GTAAAAGTACCATTAAAACATTTAGTGGAAACAGAGAAAAGTAAAGCGCCAAATGGTTGG
  ATATTGCATATACCAAGCTGTTATTTAGGTATAGCACAAGCTGCTAGAAATTATGCTGTA
  GACTTTGCAATCCAACATAGCCCTAATAGTATTGAAGGAACGATTGCAACATTGCCAACT
  GTGCAACAAAATTTAGGGAAAATGGAAACGCTATTGTTATGTGCGAGACAATTTTTATGG
  AGTACAGCAAAAGGGTATCAACAATATAAAGATGACAGTCAGATAAGAAATCCAACAAGT
  GCGAGTAAAGTGATGGTAATGAATCAAGGGCTTGAAGTGATTGATTTAGCGATGAGAATA
  GTTGGTGCTAAAAGCTTAGAAATGAATCGACCATTACAACGTTACTATCGTGACATGCGT
  GCAGGATTACACAATCCACCTATGGAAGATGCAGCATATACAAATATTGCTAAGAGTATT
  ACAGATACATTTTAA
  60
  120
  180
  240
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  360
  420
  480
  540
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  660
  720
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  840
  900
  960
  1020
  1080
  1140
  1155
  

⊟Protein

⊟General

• locus tag: SA2080 [new locus tag: SA_RS11955 ]
• symbol: SA2080
• description: hypothetical protein
• length: 384
• theoretical pI: 5.85467
• theoretical MW: 42999.8
• GRAVY: -0.246354

⊟Function

• TIGRFAM:
  Metabolism Central intermediary metabolism Sulfur metabolism sulfur acquisition oxidoreductase, SfnB family (TIGR04022; EC 1.-.-.-; HMM-score: 104.2)
  and 2 more
  pimeloyl-CoA dehydrogenase, large subunit (TIGR03204; EC 1.3.1.62; HMM-score: 34.3)
  cyclohexanecarboxyl-CoA dehydrogenase (TIGR03207; EC 1.1.1.-; HMM-score: 32.9)
• TheSEED  :
  • Acyl-CoA dehydrogenase
  Lysine fermentation  Butyryl-CoA dehydrogenase (EC 1.3.99.2)
  and 1 more
  Lysine fermentation MCB 432  Butyryl-CoA dehydrogenase (EC 1.3.99.2)
• PFAM:
  Acyl-CoA_dh (CL0087) Acyl-CoA_dh_2; Acyl-CoA dehydrogenase, C-terminal domain (PF08028; HMM-score: 51.2)
  no clan defined Acyl-CoA_dh_M; Acyl-CoA dehydrogenase, middle domain (PF02770; HMM-score: 50)
  Acyl-CoA_dh (CL0087) Acyl-CoA_dh_1; Acyl-CoA dehydrogenase, C-terminal domain (PF00441; HMM-score: 44.5)
  and 1 more
  AcylCoA_ox_dh_N (CL0544) Acyl-CoA_dh_N; Acyl-CoA dehydrogenase, N-terminal domain (PF02771; HMM-score: 28)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.0164
  • TAT(Tat/SPI): 0.001229
  • LIPO(Sec/SPII): 0.002022
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15927865 NCBI
• RefSeq: NP_375398 NCBI
• UniProt: A0A0H3JNK2 UniProt

⊟Protein sequence

• MNDMDNSFLITTEIQRKWIEKFKVIRDTFKAKAEYNDQHSQFPYKNIEWLIKEGYGKLTLPKAYGGEGATIEDMVILQSFLGELDGATALSIGWHVSVVGQIYEQKLWSQDMLEQFAVEINNGALVNRAVSEAEMGSPTRGGRPSTHAVKADDGYILNGVKTYTSMSKALTHIIVAAYIEELESVGFFLVDRNLPGVEIADNWDVLGMRATESHDLILNDVKVPLKHLVETEKSKAPNGWILHIPSCYLGIAQAARNYAVDFAIQHSPNSIEGTIATLPTVQQNLGKMETLLLCARQFLWSTAKGYQQYKDDSQIRNPTSASKVMVMNQGLEVIDLAMRIVGAKSLEMNRPLQRYYRDMRAGLHNPPMEDAAYTNIAKSITDTF

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator: CymR* (repression) regulon

  CymR*

  (TF)

  important in Cysteine metabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Olga Soutourina, Sarah Dubrac, Olivier Poupel, Tarek Msadek, Isabelle Martin-Verstraete
The pleiotropic CymR regulator of Staphylococcus aureus plays an important role in virulence and stress response.
PLoS Pathog: 2010, 6(5);e1000894
[PubMed:20485570] [WorldCat.org] [DOI] (I e)