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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2077 [new locus tag: SA_RS11940 ]
  • pan locus tag?: SAUPAN005744000
  • symbol: SA2077
  • pan gene symbol?: bioY
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA2077 [new locus tag: SA_RS11940 ]
  • symbol: SA2077
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2337188..2337742
  • length: 555
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGACAACAAAACAGTTAGTATATACAGCTTTAATGACAGCGATTATCGCTATTTTAGGA
    ATGGTACCGGTAATTCCACTACCATTTTCTTCAGTACCAATTGTACTTCAAAACATTGGT
    ATTTTCTTAGCAGGTGTGATTTTAGGACGTAAATATGGCACATTAAGTGTTATCGTCTTT
    TTATTATTAGTAGTTGCTGGCTTGCCATTGTTATCAGGTGGTCGCGGTGGCATCGGTGTA
    TTCGCAGGTCCTTCAGCAGGGTTTTTACTATTATATCCAGTTGTAGCATTCATGATTGGT
    GCGATTCGTGATAGATTCATCAATGAAATTAATTTCTGGATTTTATTCGTTGGTATTTTA
    GTTTTTGGTGTTATAGCATTAGATGTTATTGGTACATTGATTATGGGCATGATTATTAAC
    ATACCATTTACGAAAGCTATTTCAATTTCATTAGCTTATTTGCCTGGTGATATATTAAAA
    GCAATTGTAGCAAGTTTGATTGGTACAGCTTTACTTAATCACTCGCAGTTTCGTCAAATT
    ATGGGATTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    555

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA2077 [new locus tag: SA_RS11940 ]
  • symbol: SA2077
  • description: hypothetical protein
  • length: 184
  • theoretical pI: 10.5944
  • theoretical MW: 19509.8
  • GRAVY: 1.35598

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Unknown substrate ECF transporter S component, folate family (TIGR04518; HMM-score: 20.8)
  • TheSEED  :
    • Biotin ECF transporter, substrate-specific component BioY
    Cofactors, Vitamins, Prosthetic Groups, Pigments Biotin Biotin biosynthesis  Substrate-specific component BioY of biotin ECF transporter
    and 1 more
    Membrane Transport Membrane Transport - no subcategory ECF class transporters  Substrate-specific component BioY of biotin ECF transporter
  • PFAM:
    Gx_transp (CL0315) BioY; BioY family (PF02632; HMM-score: 100.7)
    and 2 more
    ECF-ribofla_trS; ECF-type riboflavin transporter, S component (PF07155; HMM-score: 42.9)
    ECF_trnsprt; ECF transporter, substrate-specific component (PF12822; HMM-score: 22.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.022118
    • TAT(Tat/SPI): 0.001905
    • LIPO(Sec/SPII): 0.049425
  • predicted transmembrane helices (TMHMM): 6

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTTKQLVYTALMTAIIAILGMVPVIPLPFSSVPIVLQNIGIFLAGVILGRKYGTLSVIVFLLLVVAGLPLLSGGRGGIGVFAGPSAGFLLLYPVVAFMIGAIRDRFINEINFWILFVGILVFGVIALDVIGTLIMGMIINIPFTKAISISLAYLPGDILKAIVASLIGTALLNHSQFRQIMGLK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: BirA* (repression) regulon
    BirA*(TF)important in Biotin biosynthesis; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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