From AureoWiki
Jump to navigation Jump to search

NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1850 [new locus tag: SA_RS10620 ]
  • pan locus tag?: SAUPAN005290000
  • symbol: SA1850
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1850 [new locus tag: SA_RS10620 ]
  • symbol: SA1850
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2088036..2089115
  • length: 1080
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTGGATGATTTTTAGTGGCTTAATTGTCGGGGGACTCTTAGGTTTTGTCATGCAAAGA
    ACAAGATTTTGTTTAACAGGTGGCTTTCGAGATATGTATGTGCAAAAGAATAATAAGATG
    TTCTATGCATTATTAATCGCTATTACTATTCAAAGTATAGGATTATTGATTTTGACGGCA
    ACAGATATTTTACAAATTCCTGCACATAGTTTTCCAATATTGGGAACAATTATAGGTTCT
    TTTATTTTTGGAATTGGAATAGTATTGGCTGGAGGGTGTGCAACAGGTACTTGGTATCGC
    GCTGGTGAAGGGCTAATTGGTAGTTGGATTGCATTAGTATTATATGCTGTTACTGCAGCA
    ATCACTAAAACAGGGATTTTAAAGCCAGTAATGGATAAAATTAATCAACCAACGAATGTA
    AATAGTGATATGTCTCAAACAACTGGCATTCCGTTTTGGGGATTAGTCGTTATATTAACT
    ATAATCACCATTTTTCTAGTTGTAAGAACACTCAATAATAAGAAAGTTCGAGTTGCTGTT
    CCAAAACTAAAGCAACGTTATACAGGTATTAGATATTACCTTTTCGAAAAACGATACCAT
    CCATTTATTGCAGCAATTGTAATTGGACTTATCGCACTCTTAGCTTGGCCAATGAGTGCA
    TCAACTGGAAGAAATGACGGTTTAGGTATAACAACGCCTTCAGCAAATTTAGTACACTTT
    TTGATTACAGGTGAAACTAAATTTATTGATTGGGGTGTCTTTTTAGTTCTAGGAATTTTC
    ATTGGTTCATATATTGCAGCTAGAGGATCAAGAGAATTTAAATGGCGATTGCCAGACAAG
    ATTACAATACGAAACAGTGCCATTGGTGGCATATGTATGGGATTTGGTGCGTCAGTTGCT
    GGTGGTTGTTCTATCGGTAACGGTTTGGTTGAAACGGCAACGATGACTTGGCAAGGATGG
    ATTGCGCTAGCATCGATGATAGTTGGTGTATGGACAATGAGTCATTTTATCTTTGTTCGT
    CCAATGAAAAAAGTACAACAACAATCCGCAAAGGTTAAACAGCAAACGCAAATAGTATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1850 [new locus tag: SA_RS10620 ]
  • symbol: SA1850
  • description: hypothetical protein
  • length: 359
  • theoretical pI: 10.7059
  • theoretical MW: 39252.5
  • GRAVY: 0.600279

⊟Function[edit | edit source]

  • TIGRFAM:
    putative selenium metabolism protein, YedE family (TIGR04112; HMM-score: 51.7)
    and 2 more
    Hypothetical proteins Conserved TIGR00701 family protein (TIGR00701; HMM-score: 11.8)
    tandem five-TM protein (TIGR01218; HMM-score: 8.4)
  • TheSEED  :
    • UPF0394 inner membrane protein YeeE
  • PFAM:
    no clan defined Sulf_transp; Sulphur transport (PF04143; HMM-score: 332.7)
    and 1 more
    DUF2070; Predicted membrane protein (DUF2070) (PF09843; HMM-score: 6.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 9
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.011463
    • TAT(Tat/SPI): 0.000534
    • LIPO(Sec/SPII): 0.011773
  • predicted transmembrane helices (TMHMM): 9

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MWMIFSGLIVGGLLGFVMQRTRFCLTGGFRDMYVQKNNKMFYALLIAITIQSIGLLILTATDILQIPAHSFPILGTIIGSFIFGIGIVLAGGCATGTWYRAGEGLIGSWIALVLYAVTAAITKTGILKPVMDKINQPTNVNSDMSQTTGIPFWGLVVILTIITIFLVVRTLNNKKVRVAVPKLKQRYTGIRYYLFEKRYHPFIAAIVIGLIALLAWPMSASTGRNDGLGITTPSANLVHFLITGETKFIDWGVFLVLGIFIGSYIAARGSREFKWRLPDKITIRNSAIGGICMGFGASVAGGCSIGNGLVETATMTWQGWIALASMIVGVWTMSHFIFVRPMKKVQQQSAKVKQQTQIV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CymR* (repression) regulon
    CymR*(TF)important in Cysteine metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SA1850&oldid=87500"