COLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA1777 [new locus tag: SA_RS10215 ]
• pan locus tag^?:
• symbol: SA1777
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1777 [new locus tag: SA_RS10215 ]
• symbol: SA1777
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 2029668..2031329
• length: 1662
• essential: unknown

⊟Accession numbers

• Gene ID: 1124666 NCBI
• RefSeq: NP_375075 NCBI
• BioCyc: see SA_RS10215
• MicrobesOnline: 104101 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGATTAGTAATAAATACGTTGATGAATATATAAATTTGTGGAAACAAGGAAAGATAATT
  TTAAATAAAGAAAGAATTGATCTCTTTAATTATCTACAAAAACATATATATTCACGAGAT
  GATGTATATTTTGATGAACAGAAAATCGAGGATTGTATCAAATTTATTGAAAAATGGTAT
  TTTCCAACATTACCATTTCAAAGGTTTATCATAGCTAATATATTTCTTATAGATAAAAAT
  ACAGATGAAGCTTTCTTTACAGAATTTGCTATTTTCATGGGACGTGGAGGCGGGAAAAAC
  GGTCTAATAAGTGCTATTAGTGATTTTCTTTCTACGCCCTTACACGGAGTTAAAGAATAT
  CACATCTCCATTGTTGCTAATAGTGAAGATCAAGCAAAAACATCGTTTGATGAAATCAGA
  ACCGTTTTAATGGATAACAAACGAAATAAGACGGGTAAAACGCCAAAAGCTCCTTATGAA
  GTTAGTAAAGCAAAAATAATAAACCGTGCAACTAAATCGGTTATTCGATATAACACATCA
  AACACAAAAACCAAAGACGGTGGACGTGAGGGGTGTGTTATTTTTGATGAAATTCATTAT
  TTCTTTGGTCCTGAAATGGTAAACGTCAAACGTGGTGGATTAGGTAAAAAGAAAAATAGA
  AGAACGTTTTATATAAGTACTGATGGTTTTGTTAGAGAGGGTTATATCGATGCAATGAAG
  CACAAAATTGCAAGTGTATTAAGTGGCAAGGTTAAAAATAGTAGATTGTTTGCTTTTTAT
  TGTAAGTTAGACGATCCAAAAGAAGTTGATGACAGACAGACGTGGGAAAAGGCGAACCCA
  ATGTTACATAAACCGTTATCAGAATACGCTAAAACACTGCTAAGCACGATTGAAGAAGAA
  TATAACGATTTACCATTCAACCGTTCAAATAAGCCCGAATTCATGACTAAGCGAATGAAT
  TTGCCTGAAGTTGACCTTGAAAAAGTAATAGCACCATGGAAAGAAATACTAGCGACTAAT
  AGAGAGATACCAAATTTAGATAATCAAATGTGTATTGGTGGTTTAGACTTTGCAAACATT
  CGAGATTTTGCAAGTGTAGGGCTATTATTCCGAAAAAACGATGATTACATTTGGTTAGGA
  CATTCGTTTGTAAGACAAGGGTTTTTGGATGATGTCAAATTAGAACCTCCTATTAAAGAA
  TGGGAAAAAATGGGATTATTGACCATTGTCGATGATGATGTCATTGAAATTGAATATATA
  GTTGATTGGTTTTTAAAGGCTAGAGAAAAATATGGGCTTGAAAAAGTCATAGCTGATAAT
  TATAGAACTGATATTGTAAGACGTGCGTTTGAGGATGCTGGCATAAAACTTGAAGTACTT
  AGAAATCCAAAAGCAATACATGGATTACTTGCACCACGTATCGATACAATGTTTGCGAAA
  CATAACGTAATATATGGAGACAATCCTTTGATGCGTTGGTTTACTAATAATGTTGCAGTA
  AAGGTTAAACCCGATGGTAATAAAGAATATATTAAAAAAGATGAAAATAGAAGAAAAACC
  GATGGGTTCATGGCTTTTGTTCACGCATTATATAGAGCAGACGATATAGTAGACAAAGAC
  ATGTCTAAAGCGCTTGATGCATTAATGAGTATAGATTTCTAA
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  1662
  

⊟Protein

⊟General

• locus tag: SA1777 [new locus tag: SA_RS10215 ]
• symbol: SA1777
• description: hypothetical protein
• length: 553
• theoretical pI: 8.46683
• theoretical MW: 64307.4
• GRAVY: -0.45859

⊟Function

• TIGRFAM:
• TheSEED  :
  • Phage terminase, large subunit
• PFAM:
  P-loop_NTPase (CL0023) TerL_ATPase; Terminase large subunit, ATPase domain (PF03354; HMM-score: 60.6)
  RNase_H (CL0219) TerL_nuclease; Terminase large subunit, endonuclease domain (PF20441; HMM-score: 60.5)
  and 1 more
  P-loop_NTPase (CL0023) ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 16.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9443
  • Cytoplasmic Membrane Score: 0.0071
  • Cell wall & surface Score: 0.0001
  • Extracellular Score: 0.0486
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002105
  • TAT(Tat/SPI): 0.000095
  • LIPO(Sec/SPII): 0.000782
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15927542 NCBI
• RefSeq: NP_375075 NCBI
• UniProt: A0A0H3JMS2 UniProt

⊟Protein sequence

• MISNKYVDEYINLWKQGKIILNKERIDLFNYLQKHIYSRDDVYFDEQKIEDCIKFIEKWYFPTLPFQRFIIANIFLIDKNTDEAFFTEFAIFMGRGGGKNGLISAISDFLSTPLHGVKEYHISIVANSEDQAKTSFDEIRTVLMDNKRNKTGKTPKAPYEVSKAKIINRATKSVIRYNTSNTKTKDGGREGCVIFDEIHYFFGPEMVNVKRGGLGKKKNRRTFYISTDGFVREGYIDAMKHKIASVLSGKVKNSRLFAFYCKLDDPKEVDDRQTWEKANPMLHKPLSEYAKTLLSTIEEEYNDLPFNRSNKPEFMTKRMNLPEVDLEKVIAPWKEILATNREIPNLDNQMCIGGLDFANIRDFASVGLLFRKNDDYIWLGHSFVRQGFLDDVKLEPPIKEWEKMGLLTIVDDDVIEIEYIVDWFLKAREKYGLEKVIADNYRTDIVRRAFEDAGIKLEVLRNPKAIHGLLAPRIDTMFAKHNVIYGDNPLMRWFTNNVAVKVKPDGNKEYIKKDENRRKTDGFMAFVHALYRADDIVDKDMSKALDALMSIDF

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA1768 < SA1769 < SA1770 < SA1771 < SA1772 < SA1773 < SA1774 < SA1775 < SA1776 < SA1777 < SA1778

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications