PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA1665 [new locus tag: SA_RS09410 ]
• pan locus tag^?: SAUPAN004780000
• symbol: SA1665
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1665 [new locus tag: SA_RS09410 ]
• symbol: SA1665
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 1901505..1901969
• length: 465
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1124516 NCBI
• RefSeq: NP_374954 NCBI
• BioCyc: see SA_RS09410
• MicrobesOnline: 103980 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGGATAGACAGAGTTTTACAGATTTAATTCAAACAAAATTTAAAATGGTTCGTATAGAG
  GCTGGTTATACGCAAGATACTATGGCGCAAACAATTGGACTTTCTAAAAAGACTTTAGTA
  CAAATCGAAAAAGAGAGAGTATTACCAAACTGGACAACTTGTATTTCAATTTGTGCATTA
  TTCAGAGACTCTGAAGTATTAAACAGTACATTTGGCTGTGATCCATTAGAAATCGTTCAA
  ACAATTTCAAGAAATCACTGTGCATATCCAAACCATGCACCAACAAGTGATATTTATTGG
  AATAACATTGAGACTCGTAATGGGTACATTTTACAAAGTAACAAAGTAAGCAATATTTAT
  CGTGTATTAAATCCAGATAACCAACCAATTTTCGGTACTTCTAAAATGAGAGAAGCTGAA
  ACATACTTTAACAGAAATGCTAAAGAAGAATTAGTACATATCTAG
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  465
  

⊟Protein

⊟General

• locus tag: SA1665 [new locus tag: SA_RS09410 ]
• symbol: SA1665
• description: hypothetical protein
• length: 154
• theoretical pI: 7.38252
• theoretical MW: 17798.1
• GRAVY: -0.465584

⊟Function

• TIGRFAM:
  Signal transduction Regulatory functions DNA interactions transcriptional regulator, y4mF family (TIGR03070; HMM-score: 17.9)
  and 1 more
  putative zinc finger/helix-turn-helix protein, YgiT family (TIGR03830; HMM-score: 14.1)
• TheSEED  :
  • HTH domain protein SA1665, binds to mecA promoter region
• PFAM:
  HTH (CL0123) HTH_3; Helix-turn-helix (PF01381; HMM-score: 25.2)
  HTH_19; Helix-turn-helix domain (PF12844; HMM-score: 23.5)
  HTH_31; Helix-turn-helix domain (PF13560; HMM-score: 20.3)
  and 2 more
  Pou; Pou domain - N-terminal to homeobox domain (PF00157; HMM-score: 13.7)
  HTH_25; Helix-turn-helix domain (PF13413; HMM-score: 12.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.007743
  • TAT(Tat/SPI): 0.002454
  • LIPO(Sec/SPII): 0.001453
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15927421 NCBI
• RefSeq: NP_374954 NCBI
• UniProt: A0A0H3JN49 UniProt

⊟Protein sequence

• MDRQSFTDLIQTKFKMVRIEAGYTQDTMAQTIGLSKKTLVQIEKERVLPNWTTCISICALFRDSEVLNSTFGCDPLEIVQTISRNHCAYPNHAPTSDIYWNNIETRNGYILQSNKVSNIYRVLNPDNQPIFGTSKMREAETYFNRNAKEELVHI

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Miriam Ender, Brigitte Berger-Bächi, Nadine McCallum
A novel DNA-binding protein modulating methicillin resistance in Staphylococcus aureus.
BMC Microbiol: 2009, 9;15
[PubMed:19173709] [WorldCat.org] [DOI] (I e)