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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1347 [new locus tag: SA_RS07635 ]
  • pan locus tag?: SAUPAN004071000
  • symbol: bfmBAB
  • pan gene symbol?: bfmBAB
  • synonym: bkdAB
  • product: branched-chain alpha-keto acid dehydrogenase E1

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1347 [new locus tag: SA_RS07635 ]
  • symbol: bfmBAB
  • product: branched-chain alpha-keto acid dehydrogenase E1
  • replicon: chromosome
  • strand: -
  • coordinates: 1556807..1557790
  • length: 984
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGCTAAATTATCTTATTTAGAGGCGATACGCCAGGCACAAGACTTAGCATTACAACAA
    AATAAAGATGTGTTTATTCTTGGTGAAGATGTTGGTAAAAAAGGTGGCGTATTTGGTACT
    ACTCAAGGACTACAACAACAATATGGTGAAGACAGAGTTATCGATACGCCATTAGCTGAG
    TCAAATATTGTTGGAACTGCCATTGGTGCAGCTATGGTAGGTAAAAGACCAATTGCAGAA
    ATTCAATTTGCAGATTTTATTTTACCTGCGACAAATCAAATTATTAGTGAAGCTGCGAAA
    ATGCGTTATCGTTCAAATAATGATTGGCAATGCCCATTAACGATTCGTGCACCATTTGGT
    GGCGGTGTTCACGGAGGACTATATCATTCACAAAGTATTGAAAGTATATTTGCTTCATCA
    CCAGGTTTAACAATTGTTATTCCTTCCACACCGTATGATGCTAAAGGGCTTTTATTATCA
    TCTATCGAATCAAATGATCCAGTATTGTACTTTGAGCATAAAAAAGCTTATCGTTTCTTA
    AAGGAAGAAGTGCCAGAAGAATATTACACTGTACCTTTAGGAAAAGCAGATGTTAAACGT
    GAAGGTGAAGATTTAACAGTATTTTGTTATGGATTAATGGTCAATTATTGTTTACAAGCA
    GCGGATATTTTAGCTGCAGACGGTATCAACGTTGAAGTCGTTGATTTACGAACTGTTTAT
    CCATTAGATAAAGAAACAATTATTGATCGTGCTAAAAATACAGGTAAAGTTTTACTTGTA
    ACTGAAGATAATTTAGAAGGTAGTATTATGTCAGAAGTGTCAGCGATTATTGCAGAGCAT
    TGCTTGTTCGATTTAGATGCACCAATCATGCGTTTAGCTGCTCCAGATGTACCATCTATG
    CCATTTTCTCCTGTATTAGAAAATGAAATTATGATGAATCCAGAAAAAATCTTAAATAAA
    ATGCGTGAATTAGCAGAATTCTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    984

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1347 [new locus tag: SA_RS07635 ]
  • symbol: BfmBAB
  • description: branched-chain alpha-keto acid dehydrogenase E1
  • length: 327
  • theoretical pI: 4.42709
  • theoretical MW: 36010.1
  • GRAVY: -0.0422018

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other 1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 75.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine 1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 75.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine 1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 75.9)
    and 2 more
    2-oxoacid:acceptor oxidoreductase, alpha subunit (TIGR03710; HMM-score: 23.7)
    indolepyruvate ferredoxin oxidoreductase, alpha subunit (TIGR03336; EC 1.2.7.8; HMM-score: 20.6)
  • TheSEED  :
    • Branched-chain alpha-keto acid dehydrogenase E1 component, beta subunit (EC 1.2.4.4)
    Amino Acids and Derivatives Branched-chain amino acids Isoleucine degradation  Branched-chain alpha-keto acid dehydrogenase, E1 component, beta subunit (EC 1.2.4.4)
    and 2 more
    Amino Acids and Derivatives Branched-chain amino acids Valine degradation  Branched-chain alpha-keto acid dehydrogenase, E1 component, beta subunit (EC 1.2.4.4)
    Carbohydrates Central carbohydrate metabolism Dehydrogenase complexes  Branched-chain alpha-keto acid dehydrogenase, E1 component, beta subunit (EC 1.2.4.4)
  • PFAM:
    THDP-binding (CL0254) Transket_pyr; Transketolase, pyrimidine binding domain (PF02779; HMM-score: 155.5)
    TKC_like (CL0591) Transketolase_C; Transketolase, C-terminal domain (PF02780; HMM-score: 136.1)
    and 2 more
    PFOR_II; Pyruvate:ferredoxin oxidoreductase core domain II (PF17147; HMM-score: 24.1)
    no clan defined DUF2795; Protein of unknown function (DUF2795) (PF11387; HMM-score: 12.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00726
    • TAT(Tat/SPI): 0.000337
    • LIPO(Sec/SPII): 0.00042
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAKLSYLEAIRQAQDLALQQNKDVFILGEDVGKKGGVFGTTQGLQQQYGEDRVIDTPLAESNIVGTAIGAAMVGKRPIAEIQFADFILPATNQIISEAAKMRYRSNNDWQCPLTIRAPFGGGVHGGLYHSQSIESIFASSPGLTIVIPSTPYDAKGLLLSSIESNDPVLYFEHKKAYRFLKEEVPEEYYTVPLGKADVKREGEDLTVFCYGLMVNYCLQAADILAADGINVEVVDLRTVYPLDKETIIDRAKNTGKVLLVTEDNLEGSIMSEVSAIIAEHCLFDLDAPIMRLAAPDVPSMPFSPVLENEIMMNPEKILNKMRELAEF

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SA0033(aadD)kanamycin nucleotidyltransferase  [2] (data from MRSA252)
    SA2428(arcA)arginine deiminase  [2] (data from MRSA252)
    SA1348(bfmBAA)branched-chain alpha-keto acid dehydrogenase E1  [2] (data from MRSA252)
    SA1346(bmfBB)branched-chain alpha-keto acid dehydrogenase E2 subunit  [2] (data from MRSA252)
    SA1046(carB)carbamoyl phosphate synthase large subunit  [2] (data from MRSA252)
    SA1517(citC)isocitrate dehydrogenase  [2] (data from MRSA252)
    SA0869(fabI)enoyl-ACP reductase  [2] (data from MRSA252)
    SA1029(ftsZ)cell division protein FtsZ  [2] (data from MRSA252)
    SA0505(fus)elongation factor G  [2] (data from MRSA252)
    SA1510(gapB)glyceraldehyde 3-phosphate dehydrogenase 2  [2] (data from MRSA252)
    SA1716(gatA)aspartyl/glutamyl-tRNA amidotransferase subunit A  [2] (data from MRSA252)
    SA1715(gatB)aspartyl/glutamyl-tRNA amidotransferase subunit B  [2] (data from MRSA252)
    SA2334(mvaS)3-hydroxy-3-methylglutaryl-CoA synthase  [2] (data from MRSA252)
    SA0943-1(pdhA)pyruvate dehydrogenase E1 component subunit alpha  [2] (data from MRSA252)
    SA1938(pdp)pyrimidine-nucleoside phosphorylase  [2] (data from MRSA252)
    SA1520(pykA)pyruvate kinase  [2] (data from MRSA252)
    SA0496(rplA)50S ribosomal protein L1  [2] (data from MRSA252)
    SA0497(rplJ)50S ribosomal protein L10  [2] (data from MRSA252)
    SA1099(rpsB)30S ribosomal protein S2  [2] (data from MRSA252)
    SA2031(rpsE)30S ribosomal protein S5  [2] (data from MRSA252)
    SA2034(rpsH)30S ribosomal protein S8  [2] (data from MRSA252)
    SA0506(tuf)elongation factor Tu  [2] (data from MRSA252)
    SA0627hypothetical protein  [2] (data from MRSA252)
    SA0774hypothetical protein  [2] (data from MRSA252)
    SA0802hypothetical protein  [2] (data from MRSA252)
    SA0829hypothetical protein  [2] (data from MRSA252)
    SA1271threonine dehydratase  [2] (data from MRSA252)
    SA1349dihydrolipoamide dehydrogenase  [2] (data from MRSA252)
    SA1532hypothetical protein  [2] (data from MRSA252)
    SA1924hypothetical protein  [2] (data from MRSA252)
    SA2327pyruvate oxidase  [2] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol Microbiol: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] [DOI] (P p)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 2.19 2.20 2.21 2.22 2.23 2.24 2.25 2.26 2.27 2.28 2.29 2.30 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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