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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1338 [new locus tag: SA_RS07580 ]
  • pan locus tag?: SAUPAN004042000
  • symbol: malA
  • pan gene symbol?: malA
  • synonym:
  • product: alpha-D-1,4-glucosidase

⊟Additional information (user-provided)[edit | edit source]

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1338 [new locus tag: SA_RS07580 ]
  • symbol: malA
  • product: alpha-D-1,4-glucosidase
  • replicon: chromosome
  • strand: -
  • coordinates: 1545313..1546962
  • length: 1650
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGAATAAGCAATGGTGGAAAGAAGCAGTAGCATATCAAGTATATCCAAGAAGTTTTAAT
    GATAGTAATCACGATGGTATTGGGGATTTACCTGGAATGATTGATAAATTGGACTACTTA
    AAAGATTTAGGTATCGATGTCATTTGGCTCAGTCCAATGTTTAAATCACCTAATGATGAC
    AATGGTTATGATATTAGTGACTATCAAGAAATTATGGATGAATTTGGAACGATGGAAGAC
    TTTGATTGTTTATTAAAAGGTATTCATGATAGAGGCATGAAGCTTATTTTAGATTTAGTT
    GTAAATCATACATCTGATGAACATCCTTGGTTTATAGAATCTAAATCTAGTAAAGACAAT
    CCCAAACGTGATTGGTATATTTGGCAAGATCCAAAGCAAGATGGCTCTGAACCTAACAAC
    TGGGAAAGTATCTTTAATGGATCTACATGGGAATATGATGCTAATACTGAGCAATATTAT
    TTCCATTTATTCAGTAAAAAACAACCTGATTTGAATTGGGGTAATCCAGAAGTTAGAGAT
    GCTGTATTTGAAATGATGAACTGGTGGTTTGATAAAGGCATTGATGGATTTAGAGTAGAT
    GCAATTACGCATATTAAGAAGACGTTTGAAGCGGGTGACTTACCTGTACCTGAGGGTAAA
    ACATATGCCCCAGCATTTGATGTAGATATGAATCAGCCAGGTATACAAACTTGGTTACAA
    GAGATGAAAGATCGCTCATTAAGTAAGTATGACATTATGACTGTCGGTGAAGCGAATGGT
    GTAAGCCCTGATGATGCTGATGACTGGGTCGGGGAAGAAAATGGTAAATTTAATATGATA
    TTCCAATTTGAACATTTGGGACTGTGGAATAGTGGTGATTCTCACTTTGATGTAAATTCG
    TATAAATCTGTATTAAATAGATGGCAAAAACAACTTGAAAATAAAGGTTGGAATGCGTTG
    TTTATTGAAAATCACGACCAACCACGACGTGTATCGACGTGGGGTGACGATGACAAGTAT
    TGGTATGAATCAGCAACAAGTCATGCAGCTGTTTATTTCTTGCAACAAGGTACGCCATTC
    ATTTATCAAGGTCAAGAAATTGGTATGACGAATTATCCATTTGAAAGTATTGAAACGTTT
    AACGATGTTGCTGTAAAAAATGACTATCAAATAGTGAAAGCTCAAGGTGGAGATGTAGAC
    GCTTTACTTGCGAAATATAAAGATGAGAACCGAGATAATTCTCGCACACCAATGCAATGG
    GATGATACGTTAAATGGAGGATTTACAAATGGTGAACCGTGGTTCCCAGTGAATCTGAAT
    TATAAAACTATCAATGTTGCACAACAATTAGAAGATGAGCATTCAGTATTACAATTTTAT
    AAAGATTTAATTCAATTAAGAAAGTCTAATGATGTATACGTATATGGTCAATTTGATTTA
    GTAGATGCTGAAAATTCACAAGTTTTTGCGTACACGAGAACATTAAATGAAAAGCAAGTT
    CTTATTGTAGGTAATCTTACTAACCACGAAGCAGAATTAACTGTACCATTTGATTTAAGC
    CATGGAGAAGTAAAGCTATTTAATTATGATGCCAAAGTTAATTTAAAACAGTTACGTCCA
    TATGAAGCATGTGTTATCGAACTAAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1650


⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1338 [new locus tag: SA_RS07580 ]
  • symbol: MalA
  • description: alpha-D-1,4-glucosidase
  • length: 549
  • theoretical pI: 4.28392
  • theoretical MW: 63879.2
  • GRAVY: -0.714572

⊟Function[edit | edit source]

  • reaction:
    EC 3.2.1.1?  ExPASy
    Alpha-amylase Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units
  • TIGRFAM:
    alpha,alpha-phosphotrehalase (TIGR02403; EC 3.2.1.93; HMM-score: 597.5)
    and 7 more
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides trehalose synthase (TIGR02456; EC 5.4.99.16; HMM-score: 349.6)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides malto-oligosyltrehalose synthase (TIGR02401; EC 5.4.99.15; HMM-score: 66.4)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides malto-oligosyltrehalose trehalohydrolase (TIGR02402; EC 3.2.1.141; HMM-score: 63.1)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides 1,4-alpha-glucan branching enzyme (TIGR01515; EC 2.4.1.18; HMM-score: 29.9)
    pullulanase, extracellular (TIGR02102; HMM-score: 27.3)
    pullulanase, type I (TIGR02104; EC 3.2.1.41; HMM-score: 23.8)
    Genetic information processing Protein synthesis Other ribosome biogenesis GTPase YqeH (TIGR03597; HMM-score: 11.6)
  • TheSEED  :
    • Alpha-glucosidase (EC 3.2.1.20)
    Carbohydrates Di- and oligosaccharides Maltose and Maltodextrin Utilization  Alpha-glucosidase (EC 3.2.1.20)
  • PFAM:
    TIM_barrel (CL0036) Alpha-amylase; Alpha amylase, catalytic domain (PF00128; HMM-score: 522.7)
    and 4 more
    GHD (CL0369) Malt_amylase_C; Maltogenic Amylase, C-terminal domain (PF16657; HMM-score: 32.3)
    SusG_C; Alpha-amylase SusG C-terminal domain (PF23915; HMM-score: 26)
    TIM_barrel (CL0036) hDGE_amylase; Glycogen debranching enzyme, glucanotransferase domain (PF14701; HMM-score: 15.8)
    GHD (CL0369) Cyc-maltodext_C; Cyclo-malto-dextrinase C-terminal domain (PF10438; HMM-score: 12.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9894
    • Cytoplasmic Membrane Score: 0.0003
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.0101
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006982
    • TAT(Tat/SPI): 0.000174
    • LIPO(Sec/SPII): 0.000767
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNKQWWKEAVAYQVYPRSFNDSNHDGIGDLPGMIDKLDYLKDLGIDVIWLSPMFKSPNDDNGYDISDYQEIMDEFGTMEDFDCLLKGIHDRGMKLILDLVVNHTSDEHPWFIESKSSKDNPKRDWYIWQDPKQDGSEPNNWESIFNGSTWEYDANTEQYYFHLFSKKQPDLNWGNPEVRDAVFEMMNWWFDKGIDGFRVDAITHIKKTFEAGDLPVPEGKTYAPAFDVDMNQPGIQTWLQEMKDRSLSKYDIMTVGEANGVSPDDADDWVGEENGKFNMIFQFEHLGLWNSGDSHFDVNSYKSVLNRWQKQLENKGWNALFIENHDQPRRVSTWGDDDKYWYESATSHAAVYFLQQGTPFIYQGQEIGMTNYPFESIETFNDVAVKNDYQIVKAQGGDVDALLAKYKDENRDNSRTPMQWDDTLNGGFTNGEPWFPVNLNYKTINVAQQLEDEHSVLQFYKDLIQLRKSNDVYVYGQFDLVDAENSQVFAYTRTLNEKQVLIVGNLTNHEAELTVPFDLSHGEVKLFNYDAKVNLKQLRPYEACVIELN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: CcpA regulon, MalR (repression) regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise 
    MalR(TF)important in Maltose utilization, Maltodextrin utilization; RegPrecise 

⊟Additional information (user-provided)[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟Other information (user-provided)[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]


⊟Relevant publications[edit | edit source]

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