PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA1090 [new locus tag: SA_RS06175 ]
• pan locus tag^?: SAUPAN003539000
• symbol: lytN
• pan gene symbol^?: lytN
• synonym:
• product: LytN protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1090 [new locus tag: SA_RS06175 ]
• symbol: lytN
• product: LytN protein
• replicon: chromosome
• strand: +
• coordinates: 1233582..1234700
• length: 1119
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123921 NCBI
• RefSeq: NP_374363 NCBI
• BioCyc: see SA_RS06175
• MicrobesOnline: 103389 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  
  ATGAATAAACAACAAAGTAAAGTACGCTATTCAATTAGAAAAGTTAGTATTGGAATTTTG
  TCAATTTCAATAGGTATGTTTTTGGCATTGGGTATGTCGAACAAAGCATATGCAGATGAA
  ATTGATAAATCTAAAGATTTTACAAGAGGGTATGAGCAAAATGTATTCGCGAAATCAGAG
  TTAAATGCTAATAAAAATACGACAAAAGACAAAATAAAAAATGAAGGTGCTGTTAAAACA
  TCGGACACAAGTTTAAAGTTAGACAACAAATCAGCAATTTCAAACGGAAATGAAATTAAT
  CAAGATATAAAGATTTCAAATACTCCGAAAAACTCAAGCCAAGGTAACAATCTAGTTATT
  AATAACAATGAACCTACTAAAGAAATTAAAATTGCAAACTTGGAAGCTCAAAATTCTAAT
  CAGAAGAAAACGAATAAAGTTACTAATAATTACTTTGGTTACTACAGTTTTAGAGAAGCT
  CCAAAAACACAAATCTATACTGTAAAAAAAGGAGACACACTTAGTGCTATAGCATTAAAA
  TACAAAACTACAGTTTCAAATATTCAAAATACAAATAATATAGCAAATCCTAATTTAATA
  TTTATTGGTCAAAAATTAAAAGTGCCAATGACACCATTAGTAGAACCAAAACCAAAAACA
  GTGTCTTCAAATAATAAAAGTAATAGTAATAGCAGTACATTAAATTATTTGAAAACATTA
  GAGAATAGAGGATGGGATTTCGACGGTAGTTATGGATGGCAATGTTTCGATTTAGTTAAT
  GTATATTGGAATCATCTTTATGGTCATGGATTAAAAGGATATGGAGCTAAAGATATACCA
  TATGCAAATAATTTTAATAGTGAAGCTAAAATTTATCACAACACACCAACTTTCAAAGCT
  GAACCTGGGGACTTAGTGGTTTTTAGTGGAAGATTTGGTGGAGGATATGGTCATACAGCT
  ATTGTCTTAAATGGTGATTATGATGGAAAATTAATGAAGTTCCAAAGTTTAGATCAAAAC
  TGGAATAATGGTGGATGGCGTAAAGCAGAGGTTGCACATAAAGTTGTTCATAATTATGAA
  AATGATATGATTTTTATTAGACCATTTAAAAAAGCATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1119
  

⊟Protein

⊟General

• locus tag: SA1090 [new locus tag: SA_RS06175 ]
• symbol: LytN
• description: LytN protein
• length: 372
• theoretical pI: 10.1016
• theoretical MW: 41738.7
• GRAVY: -0.719355

⊟Function

• reaction:
  EC 3.-.-.-?  ExPASy
• TIGRFAM:
  gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 40.2)
  Cellular processes Cellular processes Sporulation and germination spore coat assembly protein SafA (TIGR02899; HMM-score: 36.6)
  and 1 more
  Cellular processes Cellular processes Sporulation and germination stage VI sporulation protein D (TIGR02907; HMM-score: 20.2)
• TheSEED  :
  • Putative cell wall hydrolase
• PFAM:
  LysM (CL0187) LysM; LysM domain (PF01476; HMM-score: 67.7)
  and 6 more
  Peptidase_CA (CL0125) CHAP; CHAP domain (PF05257; HMM-score: 38.7)
  LysM (CL0187) LysM3_LYK4_5; LYK4/5 LysM3 domain (PF23473; HMM-score: 19.2)
  no clan defined YSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 17.1)
  LysM (CL0187) LysM2_CERK1_LYK3_4_5; LYK3/4/5 LysM2 domain (PF23472; HMM-score: 15.4)
  LysM2_NFP; NFP LysM2 domain (PF23457; HMM-score: 14.6)
  LysM1_NFP_LYK; NFP/LYK LysM1 domain (PF23446; HMM-score: 10.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 9.87
  • Cellwall Score: 0.12
  • Extracellular Score: 0.01
  • Internal Helices: 0
• DeepLocPro: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0
  • Cell wall & surface Score: 0.0005
  • Extracellular Score: 0.9995
• LocateP: Secretory(released) (with CS)
  • Prediction by SwissProt Classification: Extracellular
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 1
  • N-terminally Anchored Score: -2
  • Predicted Cleavage Site: NKAYADEI
• SignalP: Signal peptide SP(Sec/SPI) length 38 aa
  • SP(Sec/SPI): 0.994665
  • TAT(Tat/SPI): 0.003738
  • LIPO(Sec/SPII): 0.000806
  • Cleavage Site: CS pos: 38-39. AYA-DE. Pr: 0.9957
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 15926830 NCBI
• RefSeq: NP_374363 NCBI
• UniProt: Q7A5Y8 UniProt

⊟Protein sequence

• MNKQQSKVRYSIRKVSIGILSISIGMFLALGMSNKAYADEIDKSKDFTRGYEQNVFAKSELNANKNTTKDKIKNEGAVKTSDTSLKLDNKSAISNGNEINQDIKISNTPKNSSQGNNLVINNNEPTKEIKIANLEAQNSNQKKTNKVTNNYFGYYSFREAPKTQIYTVKKGDTLSAIALKYKTTVSNIQNTNNIANPNLIFIGQKLKVPMTPLVEPKPKTVSSNNKSNSNSSTLNYLKTLENRGWDFDGSYGWQCFDLVNVYWNHLYGHGLKGYGAKDIPYANNFNSEAKIYHNTPTFKAEPGDLVVFSGRFGGGYGHTAIVLNGDYDGKLMKFQSLDQNWNNGGWRKAEVAHKVVHNYENDMIFIRPFKKA

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: lytN > fmhC

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications