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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1088 [new locus tag: SA_RS06165 ]
  • pan locus tag?: SAUPAN003537000
  • symbol: sucC
  • pan gene symbol?: sucC
  • synonym:
  • product: succinyl-CoA synthetase subunit beta

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1088 [new locus tag: SA_RS06165 ]
  • symbol: sucC
  • product: succinyl-CoA synthetase subunit beta
  • replicon: chromosome
  • strand: +
  • coordinates: 1231259..1232425
  • length: 1167
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAATATCCACGAGTATCAAGGTAAAGAAATATTTCGTTCAATGGGCGTTGCAGTTCCA
    GAAGGACGAGTAGCATTTACTGCTGAAGAAGCGGTGGAGAAAGCAAAAGAATTAAATTCT
    GATGTATATGTTGTAAAAGCACAAATTCATGCTGGAGGTAGAGGTAAAGCAGGCGGAGTA
    AAAATTGCTAAATCTTTATCTGAGGTAGAAACATATGCAAAAGAATTATTAGGGAAAACT
    TTGGTGACACATCAAACTGGTCCAGAAGGTAAAGAAATTAAACGTTTATATATCGAAGAA
    GGTTGTGCTATTCAAAAAGAATATTACGTTGGATTCGTTATTGATCGTGCGACTGACCAA
    GTAACATTGATGGCGTCTGAAGAAGGGGGCACTGAGATTGAAGAAGTTGCTGCGAAGACT
    CCTGAAAAGATCTTCAAAGAAACTATCGATCCAGTAATCGGACTTTCACCATTCCAAGCA
    AGACGAATTGCGTTTAATATTAATATTCCTAAAGAATCTGTTAACAAAGCTGCTAAATTC
    TTATTAGCACTTTATAATGTATTCATTGAAAAAGATTGTTCAATCGTAGAAATCAACCCA
    TTAGTTACAACAGCTGATGGTGATGTATTGGCATTAGATGCTAAAATTAATTTTGATGAT
    AATGCATTATTCAGACATAAAGATGTTGTAGAATTACGTGATTTAGAAGAAGAAGATCCG
    AAAGAGATTGAAGCGTCTAAACATGATTTATCATACATTGCATTAGATGGTGACATCGGA
    TGTATGGTTAATGGTGCAGGTTTAGCCATGGCAACAATGGATACGATTAATCATTTCGGT
    GGAAACCCAGCCAATTTCTTAGATGCAGGCGGAAGCGCTACTAGAGAAAAAGTAACTGAA
    GCATTTAAAATCATTTTAGGTGATGAAAATGTTAAAGGTATTTTTGTAAACATTTTCGGT
    GGCATTATGAAATGTGATGTTATCGCAGAAGGTATCGTTGAAGCTGTAAAAGAAGTAGAT
    TTAACTTTACCACTAGTTGTACGCTTAGAAGGTACAAATGTTGAGTTAGGTAAAAAAATC
    TTAAAAGACTCAGGATTAGCAATTGAACCAGCAGCAACAATGGCTGAAGGTGCACAAAAA
    ATTGTTAAACTAGTCAAAGAAGCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1167

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1088 [new locus tag: SA_RS06165 ]
  • symbol: SucC
  • description: succinyl-CoA synthetase subunit beta
  • length: 388
  • theoretical pI: 4.63116
  • theoretical MW: 42056
  • GRAVY: -0.0510309

⊟Function[edit | edit source]

  • reaction:
    EC 6.2.1.5?  ExPASy
    Succinate--CoA ligase (ADP-forming) ATP + succinate + CoA = ADP + phosphate + succinyl-CoA
  • TIGRFAM:
    Metabolism Energy metabolism TCA cycle succinate-CoA ligase, beta subunit (TIGR01016; EC 6.2.1.-; HMM-score: 545.1)
    and 3 more
    acetyl coenzyme A synthetase (ADP forming), alpha domain (TIGR02717; EC 6.2.1.13; HMM-score: 21.8)
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification putative protein-(glutamine-N5) methyltransferase, unknown substrate-specific (TIGR03704; EC 2.1.1.-; HMM-score: 17.4)
    Cellular processes Cellular processes Biosynthesis of natural products cyanophycin synthetase (TIGR02068; EC 6.3.2.29,6.3.2.30; HMM-score: 15.3)
  • TheSEED  :
    • Succinyl-CoA ligase [ADP-forming] beta chain (EC 6.2.1.5)
    Carbohydrates Central carbohydrate metabolism Dehydrogenase complexes  Succinyl-CoA ligase [ADP-forming] beta chain (EC 6.2.1.5)
    and 2 more
    Carbohydrates Central carbohydrate metabolism TCA Cycle  Succinyl-CoA ligase [ADP-forming] beta chain (EC 6.2.1.5)
    Carbohydrates One-carbon Metabolism Serine-glyoxylate cycle  Succinyl-CoA ligase [ADP-forming] beta chain (EC 6.2.1.5)
  • PFAM:
    ATP-grasp (CL0179) ATP-grasp_2; ATP-grasp domain (PF08442; HMM-score: 279.1)
    and 4 more
    Succ_CoA_synth (CL0506) Ligase_CoA; CoA-ligase (PF00549; HMM-score: 84.9)
    ATP-grasp (CL0179) ATP-grasp_5; ATP-grasp domain (PF13549; HMM-score: 40.2)
    GARS_A; Phosphoribosylglycinamide synthetase, ATP-grasp (A) domain (PF01071; HMM-score: 17.4)
    Succ_CoA_synth (CL0506) Citrate_bind; ATP citrate lyase citrate-binding (PF16114; HMM-score: 12.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.013326
    • TAT(Tat/SPI): 0.002972
    • LIPO(Sec/SPII): 0.002325
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNIHEYQGKEIFRSMGVAVPEGRVAFTAEEAVEKAKELNSDVYVVKAQIHAGGRGKAGGVKIAKSLSEVETYAKELLGKTLVTHQTGPEGKEIKRLYIEEGCAIQKEYYVGFVIDRATDQVTLMASEEGGTEIEEVAAKTPEKIFKETIDPVIGLSPFQARRIAFNINIPKESVNKAAKFLLALYNVFIEKDCSIVEINPLVTTADGDVLALDAKINFDDNALFRHKDVVELRDLEEEDPKEIEASKHDLSYIALDGDIGCMVNGAGLAMATMDTINHFGGNPANFLDAGGSATREKVTEAFKIILGDENVKGIFVNIFGGIMKCDVIAEGIVEAVKEVDLTLPLVVRLEGTNVELGKKILKDSGLAIEPAATMAEGAQKIVKLVKEA

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SA0627hypothetical protein  [1] (data from MRSA252)
    SA0829hypothetical protein  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J Microbiol Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)

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