PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0965 [new locus tag: SA_RS05465 ]
• pan locus tag^?: SAUPAN003343000
• symbol: ctaB
• pan gene symbol^?: ctaB
• synonym:
• product: protoheme IX farnesyltransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0965 [new locus tag: SA_RS05465 ]
• symbol: ctaB
• product: protoheme IX farnesyltransferase
• replicon: chromosome
• strand: +
• coordinates: 1096182..1097096
• length: 915
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123791 NCBI
• RefSeq: NP_374234 NCBI
• BioCyc: see SA_RS05465
• MicrobesOnline: 103260 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  901
  
  ATTATGAGCAAAGAGCATACTTTGTCACAAAATATTAGCAGAGTTAACTTCAAAGAATTG
  CAACAGATAATTAAAATGGGACTTGTTCAAGGTAACTTAATTCCGGCTTTTGCGGGCGCA
  TGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCAATACCTCAAATTTTATTA
  ATGCTATTTGGATCTACTTTAATTATGGGTGGCGCATGTGCGTTAAATAATTATTACGAC
  CAAGATATTGATCGTATTATGCCTAGTAAACAAAATAGACCAACTGTAAATAATAGAATT
  ACAGATCAAAATTTATTACTATTAAGTTTTGGTATGATGTTAGTTGGAGAAATTTGTTTG
  TTTTTATTGAATATACCATCAGGCGTACTTGGTCTTATGGGGATTGTAGGTTATGTGTCT
  TATTACTCAATATGGTCTAAAAGACATACAACATGGAACACAGTGATTGGGAGTTTTCCT
  GGAGCAGTACCACCACTAATTGGATGGGTTGCAATTGAAGGACAAATTAGTTTAACAGCG
  ATTGCGCTGTTTTTAGTTGTATTTTGTTGGCAACCAATTCATTTTTATGCCTTAGCTATT
  AAACGTAAAGATGAATATGCACTTGCAAATATTCCAATGTTACCATCAGTTAAAGGCTTT
  AAACGTACACGTGTCAGTATGTTTATCTGGTTGATTATTTTATTGCCAGTACCTTTATTA
  CTAATAAATTTAGGTGTCGTATTCGTAGTGTTAGCTACATTATTAAATTTAGGATGGATT
  GCATTAGGTTTAACAACATTTAAGAAAAATTCAGATCAAACAAAATGGGCAACACAAATG
  TTTATATATTCACTAAATTATTTAGTGATCTTTTTCGTGTTAGCTGTAATTGTTTCATTA
  CTTACTTTGATCTAG
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⊟Protein

⊟General

• locus tag: SA0965 [new locus tag: SA_RS05465 ]
• symbol: CtaB
• description: protoheme IX farnesyltransferase
• length: 304
• theoretical pI: 9.93909
• theoretical MW: 33989.6
• GRAVY: 0.739474

⊟Function

• reaction:
  EC 2.5.1.-?  ExPASy
• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin protoheme IX farnesyltransferase (TIGR01473; EC 2.5.1.-; HMM-score: 318.5)
  and 2 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 4-hydroxybenzoate polyprenyl transferase (TIGR01474; EC 2.5.1.-; HMM-score: 62.1)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone putative 4-hydroxybenzoate polyprenyltransferase (TIGR01475; EC 2.5.1.-; HMM-score: 39)
• TheSEED  :
  • Heme O synthase, protoheme IX farnesyltransferase (EC 2.5.1.-) COX10-CtaB
  Respiration Respiration - no subcategory Biogenesis of cytochrome c oxidases  Heme O synthase, protoheme IX farnesyltransferase (EC 2.5.1.-) COX10-CtaB
  and 1 more
  Stress Response Stress Response - no subcategory Flavohaemoglobin  Heme O synthase, protoheme IX farnesyltransferase (EC 2.5.1.-) COX10-CtaB
• PFAM:
  Terp_synthase (CL0613) UbiA; UbiA prenyltransferase family (PF01040; HMM-score: 200.9)
  and 1 more
  no clan defined DUF4577; Domain of unknown function (DUF4577) (PF15145; HMM-score: 12.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 9
• DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.9996
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0004
• LocateP: Multi-transmembrane(Lipid modified N-termini)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPII)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: MGGACAL
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.008817
  • TAT(Tat/SPI): 0.00083
  • LIPO(Sec/SPII): 0.00228
• predicted transmembrane helices (TMHMM): 8

⊟Accession numbers

• GI: 15926701 NCBI
• RefSeq: NP_374234 NCBI
• UniProt: Q7A664 UniProt

⊟Protein sequence

• MMSKEHTLSQNISRVNFKELQQIIKMGLVQGNLIPAFAGAWLAVVMTNHSFLSSIPQILLMLFGSTLIMGGACALNNYYDQDIDRIMPSKQNRPTVNNRITDQNLLLLSFGMMLVGEICLFLLNIPSGVLGLMGIVGYVSYYSIWSKRHTTWNTVIGSFPGAVPPLIGWVAIEGQISLTAIALFLVVFCWQPIHFYALAIKRKDEYALANIPMLPSVKGFKRTRVSMFIWLIILLPVPLLLINLGVVFVVLATLLNLGWIALGLTTFKKNSDQTKWATQMFIYSLNYLVIFFVLAVIVSLLTLI

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: ctaB > SA0966

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications