PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0628 [new locus tag: SA_RS03600 ]
• pan locus tag^?: SAUPAN002556000
• symbol: SA0628
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0628 [new locus tag: SA_RS03600 ]
• symbol: SA0628
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 724337..725551
• length: 1215
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123435 NCBI
• RefSeq: NP_373883 NCBI
• BioCyc: see SA_RS03600
• MicrobesOnline: 102909 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  
  ATGTTTGCAGCGTTATTACAAATAAAGAATTATAAACTCTTTGTTGCTAATATGTTTCTA
  CTAGGTATGGGTATTGCGGTTACGGTCCCATATCTTGTTCTTTTTGCAACTAAAGATTTA
  GGTATGACAACAAATCAGTATGGATTACTTCTAGCATCTGCAGCGATTAGCCAGTTTACA
  GTAAATTCAATTATTGCTAGATTTTCGGATACGCATCACTTTAATAGAAAAATTATTATT
  ATTCTCGCATTATTAATGGGTGCGCTTGGTTTTTCAATATACTTTTTTGTAGATACAATC
  TGGTTATTCATATTACTATATGCGATTTTCCAAGGATTATTTGCACCAGCAATGCCCCAA
  CTTTACGCATCTGCTAGAGAATCTATCAATGTTTCAAGCTCTAAAGATAGAGCTCAATTT
  GCCAACACAGTATTACGTTCAATGTTCTCATTGGGCTTTTTATTTGGTCCATTTATTGGT
  GCCCAATTAATCGGTTTAAAAGGCTATGCTGGATTGTTTGGTGGAACAATAAGTATCATT
  TTATTTACTTTAGTACTTCAAGTGTTTTTCTATAAGGATTTAAACATTAAACACCCTATT
  AGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATGTTTAAAGACAAAACGCTTTTA
  TTACCATTTATTGCATTTATTTTATTACACATTGGACAATGGATGTATACGATGAATATG
  CCTTTATTTGTTACTGATTATTTAAAAGAAAATGAGCAACATGTCGGTTATTTAGCTAGT
  TTATGTGCTGGTTTAGAAGTGCCATTTATGATCATTCTTGGCGTTTTATCATCTAGATTA
  CATACTCGAACATTGTTGATTTATGGAGCGATTTTTGGTGGTTTATTCTACTTCAGCATT
  GGGGTATTTAAAAACTTCTATATGATGTTAGCAGGACAGGTATTTTTAGCTATTTTCTTA
  GCGGTTCTTTTAGGAATTGGTATTAGTTATTTCCAAGATATCTTACCAGATTTTCCAGGA
  TACGCCTCAACACTATTTTCTAATGCAATGGTTATTGGACAGTTAGGCGGTAACCTATTA
  GGTGGTGCTATGAGTCACTGGGTAGGTTTGGAAAATGTATTTTTTGTATCAGCAGCATCA
  ATCATGTTAGGTATGATACTTATATTCTTTACTAAAAATCAAAAAATTACAAAAGAGGAT
  ATGATATCAACATGA
  60
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  1140
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  1215
  

⊟Protein

⊟General

• locus tag: SA0628 [new locus tag: SA_RS03600 ]
• symbol: SA0628
• description: hypothetical protein
• length: 404
• theoretical pI: 9.52783
• theoretical MW: 44925.3
• GRAVY: 0.816089

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids sugar efflux transporter (TIGR00899; HMM-score: 161.3)
  and 5 more
  Metabolism Transport and binding proteins Cations and iron carrying compounds H+ Antiporter protein (TIGR00900; HMM-score: 44.9)
  multidrug resistance protein (TIGR00880; HMM-score: 41.1)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 18.5)
  Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 4.6)
  Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 4.6)
• TheSEED  :
  • Sugar efflux transpoter
• PFAM:
  MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 120.4)
  and 3 more
  MFS_1_like; MFS_1 like family (PF12832; HMM-score: 28.9)
  ATG22; Vacuole effluxer Atg22 like (PF11700; HMM-score: 20.3)
  no clan defined Ilm1; Increased loss of mitochondrial DNA protein 1 (PF10311; HMM-score: 12)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 12
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.033799
  • TAT(Tat/SPI): 0.002657
  • LIPO(Sec/SPII): 0.009227
• predicted transmembrane helices (TMHMM): 12

⊟Accession numbers

• GI: 15926350 NCBI
• RefSeq: NP_373883 NCBI
• UniProt: A0A0H3JL37 UniProt

⊟Protein sequence

• MFAALLQIKNYKLFVANMFLLGMGIAVTVPYLVLFATKDLGMTTNQYGLLLASAAISQFTVNSIIARFSDTHHFNRKIIIILALLMGALGFSIYFFVDTIWLFILLYAIFQGLFAPAMPQLYASARESINVSSSKDRAQFANTVLRSMFSLGFLFGPFIGAQLIGLKGYAGLFGGTISIILFTLVLQVFFYKDLNIKHPISTQQHVEKIAPNMFKDKTLLLPFIAFILLHIGQWMYTMNMPLFVTDYLKENEQHVGYLASLCAGLEVPFMIILGVLSSRLHTRTLLIYGAIFGGLFYFSIGVFKNFYMMLAGQVFLAIFLAVLLGIGISYFQDILPDFPGYASTLFSNAMVIGQLGGNLLGGAMSHWVGLENVFFVSAASIMLGMILIFFTKNQKITKEDMIST

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA0628 > SA0629

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications