PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0642 [new locus tag: NWMN_RS03650 ]
• pan locus tag^?: SAUPAN002556000
• symbol: NWMN_0642
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0642 [new locus tag: NWMN_RS03650 ]
• symbol: NWMN_0642
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 725671..726891
• length: 1221
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5330358 NCBI
• RefSeq: YP_001331676 NCBI
• BioCyc:
• MicrobesOnline: 3706189 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
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  901
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  1021
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  1141
  1201
  
  ATGGATATGTTTGCAGCGTTATTACAAATAAAGAATTATAAACTCTTTGTTGCTAATATG
  TTTCTACTAGGTATGGGTATTGCGGTTACGGTCCCATATCTTGTTCTTTTTGCAACTAAA
  GATTTAGGTATGACAACAAATCAGTATGGATTACTTCTAGCATCTGCAGCGATTAGCCAG
  TTTACAGTAAATTCAATTATTGCTAGATTTTCGGATACGCATCACTTTAATAGAAAAATT
  ATTATTATTCTCGCATTATTAATGGGTGCGCTTGGTTTTTCAATATACTTTTTTGTAGAT
  ACAATCTGGTTATTCATATTACTATATGCGATTTTCCAAGGATTATTTGCACCAGCAATG
  CCCCAACTTTACGCATCTGCTAGAGAATCTATCAATGTTTCAAGCTCTAAAGATAGAGCT
  CAATTTGCCAACACAGTATTACGTTCAATGTTCTCATTGGGCTTTTTATTTGGTCCATTT
  ATTGGTGCCCAATTAATCGGATTAAAAGGCTATGCTGGATTGTTTGGTGGAACAATAAGT
  ATCATTTTATTTACTTTAGTACTTCAAGTGTTTTTCTATAAGGATTTAAACATTAAACAC
  CCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATGTTTAAAGACAAAACG
  CTTTTATTACCATTTATTGCATTTATTTTATTACACATTGGACAATGGATGTATACGATG
  AATATGCCTTTATTTGTTACTGATTATTTAAAAGAAAATGAACAACATGTCGGTTATTTA
  GCTAGTTTATGTGCTGGTTTAGAAGTGCCATTTATGATCATTCTTGGCGTTTTATCATCT
  AGATTACAGACTCGAACATTGTTGATTTATGGAGCGATTTTTGGTGGTTTATTCTACTTC
  AGCATTGGGGTATTTAAAAACTTCTATATGATGTTAGCAGGACAGGTGTTTTTAGCTATT
  TTCTTAGCGGTTCTTTTAGGAATTGGTATTAGTTATTTCCAAGATATCTTACCAGATTTT
  CCAGGATACGCTTCAACACTATTTTCTAATGCAATGGTTATTGGACAGTTAGGCGGTAAC
  CTATTAGGTGGTGCTATGAGTCACTGGGTAGGTTTGGAAAATGTATTTTTTGTATCAGCA
  GCATCAATCATGTTAGGTATGATACTTATATTCTTTACTAAAAATCAAAAAATTACAAAA
  GAGGATGTGATATCAACATGA
  60
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  1221
  

⊟Protein

⊟General

• locus tag: NWMN_0642 [new locus tag: NWMN_RS03650 ]
• symbol: NWMN_0642
• description: hypothetical protein
• length: 406
• theoretical pI: 9.39681
• theoretical MW: 45130.5
• GRAVY: 0.813054

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids sugar efflux transporter (TIGR00899; HMM-score: 161.2)
  and 5 more
  Metabolism Transport and binding proteins Cations and iron carrying compounds H+ Antiporter protein (TIGR00900; HMM-score: 44.8)
  multidrug resistance protein (TIGR00880; HMM-score: 41.1)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 17.1)
  Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 4)
  Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 4)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 120.4)
  and 3 more
  MFS_1_like; MFS_1 like family (PF12832; HMM-score: 29.5)
  ATG22; Vacuole effluxer Atg22 like (PF11700; HMM-score: 17.5)
  no clan defined Ilm1; Increased loss of mitochondrial DNA protein 1 (PF10311; HMM-score: 12.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 12
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.016845
  • TAT(Tat/SPI): 0.00127
  • LIPO(Sec/SPII): 0.006278
• predicted transmembrane helices (TMHMM): 12

⊟Accession numbers

• GI: 151220854 NCBI
• RefSeq: YP_001331676 NCBI
• UniProt: A0A0H3K6N1 UniProt

⊟Protein sequence

• MDMFAALLQIKNYKLFVANMFLLGMGIAVTVPYLVLFATKDLGMTTNQYGLLLASAAISQFTVNSIIARFSDTHHFNRKIIIILALLMGALGFSIYFFVDTIWLFILLYAIFQGLFAPAMPQLYASARESINVSSSKDRAQFANTVLRSMFSLGFLFGPFIGAQLIGLKGYAGLFGGTISIILFTLVLQVFFYKDLNIKHPISTQQHVEKIAPNMFKDKTLLLPFIAFILLHIGQWMYTMNMPLFVTDYLKENEQHVGYLASLCAGLEVPFMIILGVLSSRLQTRTLLIYGAIFGGLFYFSIGVFKNFYMMLAGQVFLAIFLAVLLGIGISYFQDILPDFPGYASTLFSNAMVIGQLGGNLLGGAMSHWVGLENVFFVSAASIMLGMILIFFTKNQKITKEDVIST

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_0642 > NWMN_0643

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications