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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0374 [new locus tag: SA_RS02140 ]
  • pan locus tag?: SAUPAN001995000
  • symbol: pbuX
  • pan gene symbol?: pbuX
  • synonym:
  • product: xanthine permease

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA0374 [new locus tag: SA_RS02140 ]
  • symbol: pbuX
  • product: xanthine permease
  • replicon: chromosome
  • strand: +
  • coordinates: 431708..432976
  • length: 1269
  • essential: yes DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAAAAATTTAATCCTAAGTGTTCAACATCTTTTAGCTATGTACGCAGGTGCCATCTTA
    GTTCCAATCATTGTTGGTACAAGTTTGAAGTTTACACCTGAACAAATCGCTTACTTAGTT
    ACAGTAGATATATTTATGTGTGGGGTTGCCACATTTTTACAAGCCAATAAAGTAACAGGA
    ACAGGATTACCAATCGTTCTTGGATGTACATTCACGGCTGTTGCGCCCATGATTTTAATT
    GGTCAAACGAAAGGAATAGATGTACTTTACGGTTCGCTATTTTTATCAGGGATATTAGTT
    ATTATCATCGCGCCTTTCTTTTCACATCTTGTAAAATTCTTCCCACCAGTAGTAACGGGT
    AGTGTTGTTACTATCATTGGTATCAATTTAATGCCAGTAGCAATGAATTACTTAGCTGGA
    GGTCAAGGTGCAAAGGACTATGGAGATGTTAAGAACATTTTGTTAGGTTTAATGACATTA
    ATCATTATTCTTGTTTTACAAAGATTCACAACTGGATTTATTAAGAGTATTGCCATATTA
    ATTGGACTCGTTTTAGGAACGATAGGTGCTGGCTTACTTGGGATGGTCGATATTAATCAA
    GTCAATCATGCCGGTTGGTTAGGCATCCCAGTGCCGTTTAGATTCTCAGGATTTAGCTTT
    GATGTGACATCGACGTTAGTGTTCTTTATTTTAGCTATCGTTAGTTTAATTGAGTCGACA
    GGTGTCTATCATGCGTTAAGTGAAATTACCGGTAAGAAGTTAGAAAGAAAAGATTTTCGT
    AAAGGTTATACTGCGGAAGGTCTAGCGATAGTGTTAGGTTCTATATTCAATTCATTTCCG
    TATACAGCCTATTCGCAAAATGTAGGACTTGTTTCTTTATCCGGCGCTAAGAAAAATAAT
    GTTATATACGGCATGGTCGTGTTATTACTTATATGTGGTTGTATACCTAAGCTTGGTGCA
    TTAGCAAATATCATACCGCTACCTGTGTTAGGTGGTGCGATGATAGCTATGTTTGGCATG
    GTAATGGCATATGGTGTTAGTATATTAGGACATATCGATTTTAAAAATCAAAACAATTTA
    TTAATTATTGCTGTATCAGTAGGATTAGGTACTGGTATAAGCGCTGTACCACAAGCATTT
    AAAGGTTTAGGTGAACAATTTGCATGGTTGACTCAAAACGGAATTGTTTTAGGCGCAATC
    TCTGCAATTATTCTTAATTTCTTTTTTAATGGAATAAAGTATAAACAAACGGAAGAAAAT
    GTGAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1269

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA0374 [new locus tag: SA_RS02140 ]
  • symbol: PbuX
  • description: xanthine permease
  • length: 422
  • theoretical pI: 9.51484
  • theoretical MW: 44803.4
  • GRAVY: 0.935782

⊟Function[edit | edit source]

  • TIGRFAM:
    xanthine permease (TIGR03173; HMM-score: 506.5)
    Metabolism Transport and binding proteins Nucleosides, purines and pyrimidines uracil-xanthine permease (TIGR00801; HMM-score: 410.8)
    and 2 more
    pyrimidine utilization transport protein G (TIGR03616; HMM-score: 115.9)
    putative glycosyltransferase, exosortase G-associated (TIGR03111; HMM-score: 10.1)
  • TheSEED  :
    • Xanthine permease
    • Xanthine:proton symporter PbuX
    Nucleosides and Nucleotides Purines Xanthine Metabolism in Bacteria  Xanthine permease
  • PFAM:
    APC (CL0062) Xan_ur_permease; Permease family (PF00860; HMM-score: 350)
    and 1 more
    no clan defined Chloroplast_duf; Petal formation-expressed (PF14476; HMM-score: 9.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.175968
    • TAT(Tat/SPI): 0.001447
    • LIPO(Sec/SPII): 0.0631
  • predicted transmembrane helices (TMHMM): 13

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKNLILSVQHLLAMYAGAILVPIIVGTSLKFTPEQIAYLVTVDIFMCGVATFLQANKVTGTGLPIVLGCTFTAVAPMILIGQTKGIDVLYGSLFLSGILVIIIAPFFSHLVKFFPPVVTGSVVTIIGINLMPVAMNYLAGGQGAKDYGDVKNILLGLMTLIIILVLQRFTTGFIKSIAILIGLVLGTIGAGLLGMVDINQVNHAGWLGIPVPFRFSGFSFDVTSTLVFFILAIVSLIESTGVYHALSEITGKKLERKDFRKGYTAEGLAIVLGSIFNSFPYTAYSQNVGLVSLSGAKKNNVIYGMVVLLLICGCIPKLGALANIIPLPVLGGAMIAMFGMVMAYGVSILGHIDFKNQNNLLIIAVSVGLGTGISAVPQAFKGLGEQFAWLTQNGIVLGAISAIILNFFFNGIKYKQTEENVK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: G-box (transcription termination) regulon
    G-box(RNA)important in Purine metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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