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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0349 [new locus tag: SA_RS01995 ]
  • pan locus tag?: SAUPAN001907000
  • symbol: SA0349
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA0349 [new locus tag: SA_RS01995 ]
  • symbol: SA0349
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 409131..410012
  • length: 882
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGATCAAGTTATGAGTATTATTTCATCGCTATTCGAACCATTAACTAAAATTGAAACA
    TATGAAAATATTGCAACTAAAATCGCTATGATTGTTATTTATATTATCGTAGCCCTCATA
    GTTATTAAAATACTGAATAAAATGATTGAACAGGGATTTAAGATTCAAAATAAAAGTAAA
    AAGAGTAACAAAAAGCGCTCTAAAACTTTAATATCTCTTGTTCAAAATGTAGTGAAGTAT
    ATCGTTTGGTTTATAGTTATTACGACGATTTTAAGTAAATTTGGCATTAGTGTTGAAGGT
    GTTATTGCAAGTGCTGGTGTCGTAGGTTTAGCAGTAGGTTTTGGTGCGCAGACCATTGTT
    AAAGACGTTATTACTGGATTTTTCATTATATTTGAAAGCCAATTTGATGTAGGTGATTAT
    GTTAAGATAAACAACGGTGGTACAACTGTAGCAGAGGGAACAGTTAAATCAATTGGACTC
    CGTTCAACACGTATTAATACAATATCTGGAGAACTTACAATCTTGCCAAATAGTAGCATG
    GGTGAAATAACGAATTACTCAATTACGAATGGAACAGCAATTGTTAAAATTCCAGTATCT
    GTAGATGAAAATATTGATAATGTTGAGAAGAAATTAAACAAATTATTTACATCTCTGCGT
    AGTAAATATTATTTATTTGTAAGTGATCCTGTTGTGATTGGTATTGATGCCTTTGAAGAT
    ACTAGAGTAATATTAAGGGTTTCAGCAGAAACAATACCAGGTGAAGGTTTCTCTGGTGCG
    CGTATTATTCGTAAAGAAGTACAAAAAATGTTTTTACAAGAAGGTATCAAGACACCACAA
    CCAATAATGACACCTTTTAATCATAGTGAAAACGGTGTTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    882

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA0349 [new locus tag: SA_RS01995 ]
  • symbol: SA0349
  • description: hypothetical protein
  • length: 293
  • theoretical pI: 9.86063
  • theoretical MW: 32206.5
  • GRAVY: 0.299317

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Potassium efflux system KefA protein
    • Small-conductance mechanosensitive channel
    Potassium metabolism Potassium metabolism - no subcategory Potassium homeostasis  Potassium efflux system KefA protein
  • PFAM:
    SH3 (CL0010) MS_channel_2nd; Mechanosensitive ion channel, beta-domain (PF00924; HMM-score: 65.1)
    and 8 more
    no clan defined MS_channel_1st; Mechanosensitive ion channel, transmembrane helices 2/3 (PF21088; HMM-score: 38)
    MscS_C_sf (CL0875) MS_channel_3rd; Mechanosensitive ion channel MscS, C-terminal (PF21082; HMM-score: 21.5)
    no clan defined DUF751; Protein of unknown function (DUF751) (PF05421; HMM-score: 14.2)
    DUF6688; DUF6688 N-terminal domain (PF20394; HMM-score: 12.4)
    AWPM-19; AWPM-19-like family (PF05512; HMM-score: 11.6)
    Peptidase_MA (CL0126) DUF3810; Protein of unknown function (DUF3810) (PF12725; HMM-score: 10.2)
    no clan defined DUF6019; Family of unknown function (DUF6019) (PF19483; HMM-score: 8.4)
    DUF4834; Domain of unknown function (DUF4834) (PF16118; HMM-score: 7.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 3
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.99
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0099
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00167
    • TAT(Tat/SPI): 0.00014
    • LIPO(Sec/SPII): 0.000461
  • predicted transmembrane helices (TMHMM): 3

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MDQVMSIISSLFEPLTKIETYENIATKIAMIVIYIIVALIVIKILNKMIEQGFKIQNKSKKSNKKRSKTLISLVQNVVKYIVWFIVITTILSKFGISVEGVIASAGVVGLAVGFGAQTIVKDVITGFFIIFESQFDVGDYVKINNGGTTVAEGTVKSIGLRSTRINTISGELTILPNSSMGEITNYSITNGTAIVKIPVSVDENIDNVEKKLNKLFTSLRSKYYLFVSDPVVIGIDAFEDTRVILRVSAETIPGEGFSGARIIRKEVQKMFLQEGIKTPQPIMTPFNHSENGV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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