PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0323 [new locus tag: SA_RS01855 ]
• pan locus tag^?: SAUPAN001869000
• symbol: SA0323
• pan gene symbol^?: mepA
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0323 [new locus tag: SA_RS01855 ]
• symbol: SA0323
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 380501..381856
• length: 1356
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123102 NCBI
• RefSeq: NP_373569 NCBI
• BioCyc: see SA_RS01855
• MicrobesOnline: 102595 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  
  ATGAAAGACGAACAATTATATTATTTTGAGAAATCGCCAGTATTTAAAGCGATGATGCAT
  TTCTCATTGCCAATGATGATAGGGACTTTATTAAGCGTTATTTATGGCATATTAAATATT
  TACTTTATAGGATTTTTAGAAGATAGCCACATGATTTCTGCTATCTCTCTAACACTGCCA
  GTATTTGCTATCTTAATGGGGTTGGGTAATTTATTTGGCGTTGGTGCAGGAACTTATATT
  TCACGTTTATTAGGTGCGAAAGACTATAGTAAGAGTAAATTTGTAAGTAGTTTCTCTATT
  TATGGTGGTATTGCACTAGGACTTATCGTGATTTTAGTTACTTTACCATTCAGTGATCAA
  ATCGCAGCAATTTTAGGAGCGAGAGGTGAAACGTTAGCTTTAACAAGTAATTATTTGAAA
  GTAATGTTTTTAAGTGCACCTTTTGTAATTTTGTTCTTCATATTAGAACAATTTGCACGT
  GCAATTGGGGCACCAATGATTTCTATGATTGGTATGTTAGCTAGTGTAGGCTTAAATATT
  ATTTTAGATCCAATTTTAATTTTTGGTTTTGATTTAAACGTTGTTGGTGCAGCTTTGGGT
  ACTGCAATCAGTAATGTTGCTGCTGCTCTGTTCTTTATCATTTATTTTATGAAAAATAGT
  GACGTTGTGTCAGTTAATATTAAACTTGCGAAACCTAATAAAGAAATGCTTTCTGAAATC
  TTTAAAATAGGTATTCCTGCATTTTTAATGAGTATCTTAATGGGATTCACAGGATTAGTT
  TTAAATTTATTTTTAGCACATTATGGAAACTTCGCGATTGCAAGTTATGGTATCTCATTT
  AGACTTGTGCAATTTCCAGAACTTATTATCATGGGATTATGTGAAGGTGTTGTACCACTA
  ATTGCATATAACTTTATGGCAAATAAAGGCCGTATGAAAGACGTTATCAAAGCAGTTATC
  ATGTCTATCGGCGTTATCTTTGTTGTATGTATGATTGCTGTATTTACAATTGGACATCAT
  ATGGTCGGACTATTTACTACTGATCAAGCCATTGTTGAGATGGCGACATTTATTTTGAAA
  GTAACAATGGCATCATTATTATTAAATGGTATAGGTTTCTTGTTTACTGGTATGCTTCAA
  GCGACTGGGCAAGGTCGTGGTGCTACAATTATGGCCATTTTACAAGGTGCGATTATCATT
  CCAGTATTATTTATTATGAATGCTTTGTTTGGACTAACAGGTGTCATTTGGTCATTATTA
  ATTGCTGAGTCACTTTGTGCTTTAGCAGCAATGTTAATCGTTTATTTATTACGTGATCGT
  TTGACAGTTGATACATCTGAATTAATAGAAGGTTAA
  60
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  1140
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  1260
  1320
  1356
  

⊟Protein

⊟General

• locus tag: SA0323 [new locus tag: SA_RS01855 ]
• symbol: SA0323
• description: hypothetical protein
• length: 451
• theoretical pI: 7.54225
• theoretical MW: 48815.7
• GRAVY: 1.10067

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Other MATE efflux family protein (TIGR00797; HMM-score: 194.9)
• TheSEED  :
  • Multi antimicrobial extrusion protein (Na(+)/drug antiporter), MATE family of MDR efflux pumps
  Virulence, Disease and Defense Resistance to antibiotics and toxic compounds Multidrug Resistance Efflux Pumps  Multi antimicrobial extrusion protein (Na(+)/drug antiporter), MATE family of MDR efflux pumps
• PFAM:
  MviN_MATE (CL0222) MatE; MatE (PF01554; HMM-score: 125.6)
  and 2 more
  Polysacc_synt_C; Polysaccharide biosynthesis C-terminal domain (PF14667; HMM-score: 36)
  no clan defined Imm_superinfect; Superinfection immunity protein (PF14373; HMM-score: 12)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 12
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00148
  • TAT(Tat/SPI): 0.000089
  • LIPO(Sec/SPII): 0.001544
• predicted transmembrane helices (TMHMM): 12

⊟Accession numbers

• GI: 15926036 NCBI
• RefSeq: NP_373569 NCBI
• UniProt: Q7A7N0 UniProt

⊟Protein sequence

• MKDEQLYYFEKSPVFKAMMHFSLPMMIGTLLSVIYGILNIYFIGFLEDSHMISAISLTLPVFAILMGLGNLFGVGAGTYISRLLGAKDYSKSKFVSSFSIYGGIALGLIVILVTLPFSDQIAAILGARGETLALTSNYLKVMFLSAPFVILFFILEQFARAIGAPMISMIGMLASVGLNIILDPILIFGFDLNVVGAALGTAISNVAAALFFIIYFMKNSDVVSVNIKLAKPNKEMLSEIFKIGIPAFLMSILMGFTGLVLNLFLAHYGNFAIASYGISFRLVQFPELIIMGLCEGVVPLIAYNFMANKGRMKDVIKAVIMSIGVIFVVCMIAVFTIGHHMVGLFTTDQAIVEMATFILKVTMASLLLNGIGFLFTGMLQATGQGRGATIMAILQGAIIIPVLFIMNALFGLTGVIWSLLIAESLCALAAMLIVYLLRDRLTVDTSELIEG

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA0322 > SA0323

⊟Regulation

• regulator: MepR* (repression) regulon

  MepR*

  (TF)

  important in Multidrug resistance; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Fionnuala McAleese, Peter Petersen, Alexey Ruzin, Paul M Dunman, Ellen Murphy, Steven J Projan, Patricia A Bradford
A novel MATE family efflux pump contributes to the reduced susceptibility of laboratory-derived Staphylococcus aureus mutants to tigecycline.
Antimicrob Agents Chemother: 2005, 49(5);1865-71
[PubMed:15855508] [WorldCat.org] [DOI] (P p)