PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0241 [new locus tag: SA_RS01425 ]
• pan locus tag^?: SAUPAN001123000
• symbol: ispD
• pan gene symbol^?: tarI'
• synonym:
• product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0241 [new locus tag: SA_RS01425 ]
• symbol: ispD
• product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
• replicon: chromosome
• strand: +
• coordinates: 292918..293634
• length: 717
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123020 NCBI
• RefSeq: NP_373487 NCBI
• BioCyc: see SA_RS01425
• MicrobesOnline: 102513 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGATTTATGCAGGTATTTTAGCAGGAGGTATTGGTTCGAGAATGGGGAACGTGCCATTA
  CCAAAACAATTTTTAGATATTGATAATAAACCGATTTTAATCCATACAATTGAGAAGTTC
  ATTTTAGTGAGTGAATTTAATGAGATTATTATCGCAACGCCAGCACAGTGGATTTCCCAT
  ACACAGGATATTTTAAAAAAATATAACATTACAGATCAACGTGTCAAAGTAGTTGCAGGT
  GGTACGGATCGAAACGAAACAATTATGAACATTATCGACCATATTCGCAATGTAAATGGA
  ATTAATAATGATGATGTGATTGTAACTCATGATGCCGTAAGACCATTTTTAACTCAACGT
  ATTATTAAAGAGAACATTGAAGTAGCAGCAAAATATGGTGCAGTAGATACAGTCATTGAA
  GCAATTGATACGATTGTAATGTCTAAAGATAAACAGAACATACACAGTATCCCTGTAAGG
  AATGAAATGTATCAAGGCCAAACACCACAATCATTTAATATTAAATTATTACAAGATAGT
  TATCGCGCCTTAAGTAGTGAACAAAAAGAAATCTTATCAGATGCATGTAAAATAATTGTC
  GAATCTGGACATGCAGTTAAATTGGTACGTGGAGAACTATACAACATTAAAGTGACAACA
  CCGTATGATTTAAAAGTAGCAAATGCCATTATTCAAGGTGATATTGCCGATGATTAA
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⊟Protein

⊟General

• locus tag: SA0241 [new locus tag: SA_RS01425 ]
• symbol: IspD
• description: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
• length: 238
• theoretical pI: 5.95177
• theoretical MW: 26574.4
• GRAVY: -0.0890756

⊟Function

• reaction:
  EC 2.7.7.40?  ExPASy
  D-ribitol-5-phosphate cytidylyltransferase CTP + D-ribitol 5-phosphate = diphosphate + CDP-ribitol
  EC 2.7.7.60?  ExPASy
  2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (TIGR00453; EC 2.7.7.60; HMM-score: 159.1)
  and 7 more
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides 3-deoxy-D-manno-octulosonate cytidylyltransferase (TIGR00466; EC 2.7.7.38; HMM-score: 21.1)
  molybdenum cofactor cytidylyltransferase (TIGR03310; EC 2.7.7.76; HMM-score: 21.1)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Molybdopterin molybdenum cofactor guanylyltransferase (TIGR02665; EC 2.7.7.77; HMM-score: 19.1)
  glucose-1-phosphate thymidylyltransferase (TIGR01208; EC 2.7.7.24; HMM-score: 17)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides glucose-1-phosphate cytidylyltransferase (TIGR02623; EC 2.7.7.33; HMM-score: 16.6)
  pseudaminic acid cytidylyltransferase (TIGR03584; EC 2.7.7.81; HMM-score: 13.8)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides glucose-1-phosphate thymidylyltransferase (TIGR01207; EC 2.7.7.24; HMM-score: 12.6)
• TheSEED  :
  • Sugar-phosphate cytidylyltransferase
  Cell Wall and Capsule Gram-Positive cell wall components Teichoic and lipoteichoic acids biosynthesis  2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (EC 2.7.7.60)
  and 2 more
  Cell Wall and Capsule Gram-Positive cell wall components Teichoic and lipoteichoic acids biosynthesis  ribitol-5-phosphate cytidylyltransferase (EC 2.7.7.40)
  Fatty Acids, Lipids, and Isoprenoids Isoprenoids Isoprenoid Biosynthesis  2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (EC 2.7.7.60)
• PFAM:
  GT-A (CL0110) IspD; 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (PF01128; HMM-score: 148)
  and 3 more
  NTP_transf_3; MobA-like NTP transferase domain (PF12804; HMM-score: 36.1)
  CTP_transf_3; Cytidylyltransferase (PF02348; HMM-score: 20.3)
  NTP_transferase; Nucleotidyl transferase (PF00483; HMM-score: 17.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003261
  • TAT(Tat/SPI): 0.000131
  • LIPO(Sec/SPII): 0.000908
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15925954 NCBI
• RefSeq: NP_373487 NCBI
• UniProt: P65177 UniProt

⊟Protein sequence

• MIYAGILAGGIGSRMGNVPLPKQFLDIDNKPILIHTIEKFILVSEFNEIIIATPAQWISHTQDILKKYNITDQRVKVVAGGTDRNETIMNIIDHIRNVNGINNDDVIVTHDAVRPFLTQRIIKENIEVAAKYGAVDTVIEAIDTIVMSKDKQNIHSIPVRNEMYQGQTPQSFNIKLLQDSYRALSSEQKEILSDACKIIVESGHAVKLVRGELYNIKVTTPYDLKVANAIIQGDIADD

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: ispD > SA0242 > SA0243

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications