PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0225 [new locus tag: SA_RS01320 ]
• pan locus tag^?: SAUPAN001094000
• symbol: SA0225
• pan gene symbol^?: fadD
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0225 [new locus tag: SA_RS01320 ]
• symbol: SA0225
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 270900..272111
• length: 1212
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123002 NCBI
• RefSeq: NP_373469 NCBI
• BioCyc: see SA_RS01320
• MicrobesOnline: 102495 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  
  ATGACATTTGAAAAAGAAACGGTCTTAAAAACATTATTTCCTGAAGATGTACTTAGTATT
  GCTAAAGGTTTAACAGACGGTGAAGTCGAATTTTTACAACAAGTAGATTCATTGCTAGAA
  AGTAATTACCGTGAAAATATTAATCAACATTGGATAGACGCTACTGTACCCGAGGACTAT
  TTTAAAGATCTGGGAGAATTAAATTATTTTAACAATCCATTACTTTACAAGGATCGTCCA
  AACGCCAAAATGCCTAGTCAACTATTTCAGTTTTTCATGTCTTACCTACTCGCGCGATTT
  GATATTTCCTTAGCTACCCTACTCGGTGTTCACCAAGGTTTAGGGCATAACACTTTCTAT
  TTCGGAGGTAGCAAAGAACAAATTGCGAAATATGTACCTAAATTACTGTCACATGAACTG
  CGTACATGCTTTGCTTTAACTGAACCAGAACACGGTTCGGACGTTGCGGGAGGTCTTGAA
  ACAGTCGCTGAACGCCAAGGCGATACTTGGGTTATCAATGGTGAAAAGAAATGGATTGGT
  GGTGCACATGTATCTGATGTCATTCCAGTATTCGCAGTAAATAAAAAAACTGGCAAACCC
  CATTGCTTTGTAGTCAGACCAGAACAAGATGGCGTCGATATTGAAGTCATTGATAATAAA
  ATCGCACTTCGCATTGTTCCTAACGCACTAATTAAATTAACTAATGTCAAAGTAGATGAA
  GCGGATCGCTTACAAAACATAACAAGCTTTAAAGATATTGCCAAAATTCTTTATTCAACG
  AGAGCAGGCGTTGCTTATATGGCTACAGGTGGTATGGCTGGCGCTTTACGTGCCACATTA
  GATTATGTCACTGAGCGTAAGCAATTCGGCAAACCAATTAGTAAATATCAGTTAATACAA
  GAAAAGCTAGCAATGATGCAAGGTAATTTAGCTCAAGCAATGGCAACATGTGCTCAATTA
  GCTAATATGCAAGCACATGGTGAATATGACGAGGTTGCAACTTCAACGGCGAAGATGATG
  AATGCCTTACGTTTGCGTGAGACAGTAGCTATGGGCCGCGGTATTACAGGTGGTAATGGC
  ATACTAGCTGACGATTATGATATTGCACGTTTCTTCTCTGATGCAGAAGCGATTTACACG
  TACGAAGGTACACATGAAATTAATGCCTTAGTAATTGGACGCGCTTTGACTGGAGATTCT
  GCTTTCGTATAA
  60
  120
  180
  240
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  360
  420
  480
  540
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  840
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  1020
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  1140
  1200
  1212
  

⊟Protein

⊟General

• locus tag: SA0225 [new locus tag: SA_RS01320 ]
• symbol: SA0225
• description: hypothetical protein
• length: 403
• theoretical pI: 5.30059
• theoretical MW: 44698.7
• GRAVY: -0.150868

⊟Function

• TIGRFAM:
  cyclohexanecarboxyl-CoA dehydrogenase (TIGR03207; EC 1.1.1.-; HMM-score: 73.8)
  pimeloyl-CoA dehydrogenase, small subunit (TIGR03203; EC 1.3.1.62; HMM-score: 61.4)
  and 2 more
  pimeloyl-CoA dehydrogenase, large subunit (TIGR03204; EC 1.3.1.62; HMM-score: 55.6)
  Metabolism Central intermediary metabolism Sulfur metabolism sulfur acquisition oxidoreductase, SfnB family (TIGR04022; EC 1.-.-.-; HMM-score: 36.1)
• TheSEED  :
  • Acyl-CoA dehydrogenase, long-chain specific (EC 1.3.8.8)
• PFAM:
  Acyl-CoA_dh (CL0087) Acyl-CoA_dh_1; Acyl-CoA dehydrogenase, C-terminal domain (PF00441; HMM-score: 93.9)
  and 3 more
  no clan defined Acyl-CoA_dh_M; Acyl-CoA dehydrogenase, middle domain (PF02770; HMM-score: 69.5)
  AcylCoA_ox_dh_N (CL0544) Acyl-CoA_dh_N; Acyl-CoA dehydrogenase, N-terminal domain (PF02771; HMM-score: 32.3)
  Acyl-CoA_dh (CL0087) Acyl-CoA_dh_2; Acyl-CoA dehydrogenase, C-terminal domain (PF08028; HMM-score: 31.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: FAD
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003465
  • TAT(Tat/SPI): 0.000282
  • LIPO(Sec/SPII): 0.000603
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15925936 NCBI
• RefSeq: NP_373469 NCBI
• UniProt: A0A0H3JKI4 UniProt

⊟Protein sequence

• MTFEKETVLKTLFPEDVLSIAKGLTDGEVEFLQQVDSLLESNYRENINQHWIDATVPEDYFKDLGELNYFNNPLLYKDRPNAKMPSQLFQFFMSYLLARFDISLATLLGVHQGLGHNTFYFGGSKEQIAKYVPKLLSHELRTCFALTEPEHGSDVAGGLETVAERQGDTWVINGEKKWIGGAHVSDVIPVFAVNKKTGKPHCFVVRPEQDGVDIEVIDNKIALRIVPNALIKLTNVKVDEADRLQNITSFKDIAKILYSTRAGVAYMATGGMAGALRATLDYVTERKQFGKPISKYQLIQEKLAMMQGNLAQAMATCAQLANMQAHGEYDEVATSTAKMMNALRLRETVAMGRGITGGNGILADDYDIARFFSDAEAIYTYEGTHEINALVIGRALTGDSAFV

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA0223 < SA0224 < SA0225 < SA0226 < SA0227

⊟Regulation

• regulator: CcpA regulon

  CcpA

  (TF)

  important in Carbon catabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications