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NCBI: 26-AUG-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0169 [new locus tag: SA_RS01020 ]
  • pan locus tag?: SAUPAN001013000
  • symbol: SA0169
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA0169 [new locus tag: SA_RS01020 ]
  • symbol: SA0169
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 192305..193336
  • length: 1032
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGACTTTAGAAACGCTTATCAAAGAACAATTAGATCCTCATTTAGTAGAAGTTGATGAA
    GGGACGTATTATCCGAGAACATTTATTCAACAATTATTTGTAGATGGTTATTTCGGTGAG
    GCGGCATTGAGAAAAAATGCTGAAGTAATCGAAGCTGTATCGCAGTCTTGTTTGACAACA
    GGATTTTGTTTATGGTGCCAATTAGCTTTTTCAACGTATTTAGAAAATGCCACGCAGCCA
    CATTTAAATAATGACTTACAACAGCAATTGTTATCTGGAGAAATATTAGGTGCTACCGGA
    TTGTCTAATCCGATGAAGTCATTTAATGATTTAGAAAAGTTGAACCTTGAACACACTTAT
    GTTGATGGACAATTGGTTGTCAGTGGACGTATGCCAGCTGTAAGTAATATTCAAGAAGAC
    CATTATTTTGGTGCGATTTCGAAACATGAATCATCAGATGAATTTGTCATGTTCATTCTA
    CGTGCCAATCAAGATGGTATCACTCTCGTTGAAAAAACCAATTTTTTAGGGGTCAACGGG
    TCAGCAACGTATCAAATCACATTGAATCAAGTCGTAGTGCCACAATCACAAATCATCACG
    CATGATGCGAAGCAGTTTGCGGCAACTATTCGCCCGCAATTTATTGCTTACCAAATTCCA
    ATAGGATTAGGCTCAATTAAAAGTTCTTTAGAGTTAATTGATGCATTTTCAAATGCGCAA
    AACGGAATAAATCAATATTTAGAGTATGATGTTGAAGCTTTTAAAAAACGTTATCGTCAA
    CTTAGAGAGGAATATTATGCAATATTAGATGACGGTAACTTAACTTCACATTTAAGTGAA
    TTAATATCATTGAAGAAGGACATTGGCTATTTATTGTTAGATGTAAATCAAGCTTCTGTC
    GTCAATGGTGGTTCAAGAGCGTACACACCATATTCGCCACAAGTTCGCAAGTTAAAAGAA
    GGATTCTTCTTCGCAGCATTAACGCCGACATTAAGACATTTAGGTAAACTTGAAGCAGAG
    TTGAAGGGGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1032

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA0169 [new locus tag: SA_RS01020 ]
  • symbol: SA0169
  • description: hypothetical protein
  • length: 343
  • theoretical pI: 4.86057
  • theoretical MW: 38422.2
  • GRAVY: -0.16793

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Central intermediary metabolism Sulfur metabolism sulfur acquisition oxidoreductase, SfnB family (TIGR04022; EC 1.-.-.-; HMM-score: 21.1)
  • TheSEED  :
    • Acyl-CoA dehydrogenase, short-chain specific (EC 1.3.99.2)
    Amino Acids and Derivatives Branched-chain amino acids Isoleucine degradation  Acyl-CoA dehydrogenase, short-chain specific (EC 1.3.99.2)
  • PFAM:
    no clan defined Acyl-CoA_dh_M; Acyl-CoA dehydrogenase, middle domain (PF02770; HMM-score: 15.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006303
    • TAT(Tat/SPI): 0.000198
    • LIPO(Sec/SPII): 0.000807
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MTLETLIKEQLDPHLVEVDEGTYYPRTFIQQLFVDGYFGEAALRKNAEVIEAVSQSCLTTGFCLWCQLAFSTYLENATQPHLNNDLQQQLLSGEILGATGLSNPMKSFNDLEKLNLEHTYVDGQLVVSGRMPAVSNIQEDHYFGAISKHESSDEFVMFILRANQDGITLVEKTNFLGVNGSATYQITLNQVVVPQSQIITHDAKQFAATIRPQFIAYQIPIGLGSIKSSLELIDAFSNAQNGINQYLEYDVEAFKKRYRQLREEYYAILDDGNLTSHLSELISLKKDIGYLLLDVNQASVVNGGSRAYTPYSPQVRKLKEGFFFAALTPTLRHLGKLEAELKG

Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator: CymR* (repression) regulon
    CymR*(TF)important in Cysteine metabolism; RegPrecise 

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]