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NCBI: 26-AUG-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus N315
- locus tag: SA0169 [new locus tag: SA_RS01020 ]
- pan locus tag?: SAUPAN001013000
- symbol: SA0169
- pan gene symbol?: —
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SA0169 [new locus tag: SA_RS01020 ]
- symbol: SA0169
- product: hypothetical protein
- replicon: chromosome
- strand: +
- coordinates: 192305..193336
- length: 1032
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 1122945 NCBI
- RefSeq: NP_373412 NCBI
- BioCyc: see SA_RS01020
- MicrobesOnline: 102438 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1021ATGACTTTAGAAACGCTTATCAAAGAACAATTAGATCCTCATTTAGTAGAAGTTGATGAA
GGGACGTATTATCCGAGAACATTTATTCAACAATTATTTGTAGATGGTTATTTCGGTGAG
GCGGCATTGAGAAAAAATGCTGAAGTAATCGAAGCTGTATCGCAGTCTTGTTTGACAACA
GGATTTTGTTTATGGTGCCAATTAGCTTTTTCAACGTATTTAGAAAATGCCACGCAGCCA
CATTTAAATAATGACTTACAACAGCAATTGTTATCTGGAGAAATATTAGGTGCTACCGGA
TTGTCTAATCCGATGAAGTCATTTAATGATTTAGAAAAGTTGAACCTTGAACACACTTAT
GTTGATGGACAATTGGTTGTCAGTGGACGTATGCCAGCTGTAAGTAATATTCAAGAAGAC
CATTATTTTGGTGCGATTTCGAAACATGAATCATCAGATGAATTTGTCATGTTCATTCTA
CGTGCCAATCAAGATGGTATCACTCTCGTTGAAAAAACCAATTTTTTAGGGGTCAACGGG
TCAGCAACGTATCAAATCACATTGAATCAAGTCGTAGTGCCACAATCACAAATCATCACG
CATGATGCGAAGCAGTTTGCGGCAACTATTCGCCCGCAATTTATTGCTTACCAAATTCCA
ATAGGATTAGGCTCAATTAAAAGTTCTTTAGAGTTAATTGATGCATTTTCAAATGCGCAA
AACGGAATAAATCAATATTTAGAGTATGATGTTGAAGCTTTTAAAAAACGTTATCGTCAA
CTTAGAGAGGAATATTATGCAATATTAGATGACGGTAACTTAACTTCACATTTAAGTGAA
TTAATATCATTGAAGAAGGACATTGGCTATTTATTGTTAGATGTAAATCAAGCTTCTGTC
GTCAATGGTGGTTCAAGAGCGTACACACCATATTCGCCACAAGTTCGCAAGTTAAAAGAA
GGATTCTTCTTCGCAGCATTAACGCCGACATTAAGACATTTAGGTAAACTTGAAGCAGAG
TTGAAGGGGTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SA0169 [new locus tag: SA_RS01020 ]
- symbol: SA0169
- description: hypothetical protein
- length: 343
- theoretical pI: 4.86057
- theoretical MW: 38422.2
- GRAVY: -0.16793
⊟Function[edit | edit source]
- TIGRFAM: Central intermediary metabolism Sulfur metabolism sulfur acquisition oxidoreductase, SfnB family (TIGR04022; EC 1.-.-.-; HMM-score: 21.1)
- TheSEED :
- Acyl-CoA dehydrogenase, short-chain specific (EC 1.3.99.2)
- PFAM: no clan defined Acyl-CoA_dh_M; Acyl-CoA dehydrogenase, middle domain (PF02770; HMM-score: 15.7)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.006303
- TAT(Tat/SPI): 0.000198
- LIPO(Sec/SPII): 0.000807
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MTLETLIKEQLDPHLVEVDEGTYYPRTFIQQLFVDGYFGEAALRKNAEVIEAVSQSCLTTGFCLWCQLAFSTYLENATQPHLNNDLQQQLLSGEILGATGLSNPMKSFNDLEKLNLEHTYVDGQLVVSGRMPAVSNIQEDHYFGAISKHESSDEFVMFILRANQDGITLVEKTNFLGVNGSATYQITLNQVVVPQSQIITHDAKQFAATIRPQFIAYQIPIGLGSIKSSLELIDAFSNAQNGINQYLEYDVEAFKKRYRQLREEYYAILDDGNLTSHLSELISLKKDIGYLLLDVNQASVVNGGSRAYTPYSPQVRKLKEGFFFAALTPTLRHLGKLEAELKG
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: SA0166 > SA0167 > SA0168 > SA0169
⊟Regulation[edit | edit source]
- regulator: CymR* (repression) regulon
CymR* (TF) important in Cysteine metabolism; RegPrecise
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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