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NCBI: 26-AUG-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0119 [new locus tag: SA_RS00750 ]
  • pan locus tag?: SAUPAN000922000
  • symbol: SA0119
  • pan gene symbol?: sbnH
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA0119 [new locus tag: SA_RS00750 ]
  • symbol: SA0119
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 138019..139221
  • length: 1203
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGCGTATAGTTCAACCTGTTATTGAACAATTAAAAGCACAATCTCATCCAGTTTGTCAT
    TATATCTATGATTTAGTCGGACTGGAACATCATTTGCAACATATTACATCGTCATTGCCG
    AGTAATTGTCAAATGTACTATGCAATGAAAGCAAATAGTGAACGAACAATCCTAGATACA
    ATTAGTCAGTATGTTGAAGGATTCGAAGTTGCATCTCAAGGTGAAATAGCAAAAGGTCTT
    GCTTTTAAACCAGCAAATCATATTATTTTTGGTGGCCCTGGTAAGACAGACGAGGAACTA
    AGATATGCAGTAAGTGAAGGTGTTCAGCGTATTCATGTTGAAAGTATGCATGAATTACAA
    CGGCTAAATGCCATCTTAGAAGATGAAGATAAGACACAACACATTTTATTGCGTGTTAAT
    TTAGCAGGACCATTTCCCAATGCAACGTTGCATATGGCAGGACGCCCAACACAATTTGGT
    ATTTCTGAAGACGAAGTTGATGATGTCATTGAAGCTGCGCTAGTAATGCCAAATATTCAT
    CTAGATGGCTTTCATTTTCATTCTATTTCTAACAATTTAGACTCGAATTTACATGTCGAT
    GTAGTGAAACTTTATTTTAAAAAAGCAAAATCATGGTCTGAAAAACATCGATTTCCACTC
    AAACATATCAATCTTGGTGGTGGCATAGGCGTTAACTATGCAGATTTAACTAGCCAATTT
    GAGTGGGATAATTTTGTAGAAAATTTTAAAACACTTATCGTTGAGCAAGAAATGGAAGAT
    GTGACATTGAACTTTGAATGTGGGCGCTTTATTGTGGCACATATTGGTTACTATGTGACA
    GAAGTGCTAGATATTAAGAAAGTGCATGGTGCTTGGTATGCCATTTTAAGAGGAGGTACG
    CAACAATTTAGACTGCCGGTATCTTGGCAGCATAACCATCCTTTTGAAATTTATCGTTAT
    AAGGACAATCCATATTCATTTGAAAAAGTTTCAATTTCGAGACAGGACACAACGTTAGTC
    GGTCAATTATGTACACCGAAAGATGTCTTTGCTAGAGAAGTACAGATAGACGCAATCAGT
    ACAGGCGACGTTATTGTTTTCAAATATGCAGGTGCATACGGATGGTCTATTTCACATCAC
    GATTTCTTAAGCCATCCACATCCTGAATTTATTTATTTAACGCAAACAAAGGAGGATGAA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1203

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA0119 [new locus tag: SA_RS00750 ]
  • symbol: SA0119
  • description: hypothetical protein
  • length: 400
  • theoretical pI: 6.06568
  • theoretical MW: 45707.4
  • GRAVY: -0.2825

Function[edit | edit source]

  • TIGRFAM:
    pyridoxal-dependent decarboxylase, exosortase A system-associated (TIGR03099; HMM-score: 250.8)
    Metabolism Amino acid biosynthesis Aspartate family diaminopimelate decarboxylase (TIGR01048; EC 4.1.1.20; HMM-score: 203.3)
    and 2 more
    Metabolism Central intermediary metabolism Polyamine biosynthesis arginine decarboxylase (TIGR01273; EC 4.1.1.19; HMM-score: 37.8)
    Metabolism Central intermediary metabolism Polyamine biosynthesis carboxynorspermidine decarboxylase (TIGR01047; HMM-score: 32.5)
  • TheSEED  :
    • 2-[(L-alanin-3-ylcarbamoyl)methyl]-2-hydroxybutanedioate decarboxylase (EC 4.1.1.117)
    Iron acquisition and metabolism Siderophores Siderophore Staphylobactin  Siderophore staphylobactin biosynthesis protein SbnH
  • PFAM:
    TIM_barrel (CL0036) Orn_Arg_deC_N; Pyridoxal-dependent decarboxylase, pyridoxal binding domain (PF02784; HMM-score: 124.3)
    and 2 more
    no clan defined Orn_DAP_Arg_deC; Pyridoxal-dependent decarboxylase, C-terminal sheet domain (PF00278; HMM-score: 51.2)
    TIM_barrel (CL0036) Ala_racemase_N; Alanine racemase, N-terminal domain (PF01168; HMM-score: 24.4)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006605
    • TAT(Tat/SPI): 0.000357
    • LIPO(Sec/SPII): 0.001013
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MRIVQPVIEQLKAQSHPVCHYIYDLVGLEHHLQHITSSLPSNCQMYYAMKANSERTILDTISQYVEGFEVASQGEIAKGLAFKPANHIIFGGPGKTDEELRYAVSEGVQRIHVESMHELQRLNAILEDEDKTQHILLRVNLAGPFPNATLHMAGRPTQFGISEDEVDDVIEAALVMPNIHLDGFHFHSISNNLDSNLHVDVVKLYFKKAKSWSEKHRFPLKHINLGGGIGVNYADLTSQFEWDNFVENFKTLIVEQEMEDVTLNFECGRFIVAHIGYYVTEVLDIKKVHGAWYAILRGGTQQFRLPVSWQHNHPFEIYRYKDNPYSFEKVSISRQDTTLVGQLCTPKDVFAREVQIDAISTGDVIVFKYAGAYGWSISHHDFLSHPHPEFIYLTQTKEDE

Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator: Fur* (repression) regulon
    Fur*(TF)important in Iron homeostasis; RegPrecise 

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]

Suzanne E Dale, Amanda Doherty-Kirby, Gilles Lajoie, David E Heinrichs
Role of siderophore biosynthesis in virulence of Staphylococcus aureus: identification and characterization of genes involved in production of a siderophore.
Infect Immun: 2004, 72(1);29-37
[PubMed:14688077] [WorldCat.org] [DOI] (P p)