PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS14080 [old locus tag: NWMN_2450 ]
• pan locus tag^?: SAUPAN006212000
• symbol: NWMN_RS14080
• pan gene symbol^?: feoB
• synonym:
• product: ferrous iron transporter B

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS14080 [old locus tag: NWMN_2450 ]
• symbol: NWMN_RS14080
• product: ferrous iron transporter B
• replicon: chromosome
• strand: -
• coordinates: 2693594..2695588
• length: 1995
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (2693594..2695588) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_2450

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  1561
  1621
  1681
  1741
  1801
  1861
  1921
  1981
  
  ATGGAAAATTATTGTATTTTAGGAAATCCTAATGTTGGTAAAACATCTTTGTTTAACGCT
  TTAACTGGTTCGTATGAATATATAGGCAACTGGAGTGGCGTAACGGTAGAGAAAAAAGTA
  GGGAAACTTAAAGAAAATGTAGGACAATTAATTGATTTACCAGGTACATATGATTTATCG
  CCGATTTCTAAAGATGAAACTGTAGTGACAGACTATTTATTAAACGATTCATTTTCAGGA
  ATCATTAACATTGTTGATGCGAGTCAACTAAAAAGAAATATGCAATTAACAGTACAATTG
  TTAGAACTTAATCAACCAATATACATCGGATTAAATATGATTGATGTAGCTACAAAGCGC
  GGGATAAAAATCGATTATCATAAATTGATGAAAAAGTTAAAAACACCTATTTTTCCAGTT
  GTGGCACGTACAGGCAAAGGAACAAAATATTTACTTGGCGAAATAAAGCATCTAGGGGAA
  GGGTATCAACCGCATTTTAAAATCAATTATGGTGAGAAGATTGAAGAGACAATTAAAAAT
  ATGTGTCAAATAATAATGACAGAGACATCTCATGATAAGTATCAAGCTAGGTTTATTGCG
  ATTCAATTCTTATTAAATAACATGCAAATTGCAAACGAACTTAATTCAGAGGTTGTAAAT
  AAATTGTCGAGTTTACGTGATCAAGTTGCTGAACAAGTAGGGGCTGTATCTGTTCGTCGA
  GAGATGGAGCGTATACGCAATCATTATATTGAGACGTTATTGCAAGACGTGGTAACGTAT
  CCAGATGAAGATAAGCAATATTTCAGCTCTAGAATAGACAAGATATTAACACATAAATAT
  ATAGGGATGCCTATCTTTCTAGCAATTATGTGGTTGATCTTTCAAACTACATTTACATGG
  ATTGGCACACCTTTGTCGGATCAACTCGATGCATTTATAGGTGGTACATTTACAGATAGT
  GTGAAGACGATTATGAATTATCTTGGTGTTATACCATTTTTACAAGATTTAATTACAGAT
  GGCATTATTGCTGGTGTAGGATCAGTATTAGTCTTTGTACCACAAATTGTTGTGCTCTTT
  TTCTTTATATCTTTATTAGAAGATTCGGGTTATATGGCACGTATTGCGGTTTTAATGGAT
  CGTATTATGGAATCGTTCGGTTTAAGCGGGAAGTCTTTTATACCAATGATTATCGGATTT
  GGTTGTAATGTACCGAGTATTATGGCTGCGCGCAGTATTGAAAATGAAAAAGAGCGTTTA
  ACAACGATATTAATTGCACCATTCATGTCATGCTCTGCTAGACTGCCAGTGTATGCGTTG
  TTCGTAGGTATCTTTTTCAAAGAGAATCAATCTTTAGTAGTATTAAGCCTGTATGTTTTA
  GGTATTATTATGGCATTTTTAGTCAGTACAGTACTTACTAAGACGATTTTAAAAAATGAT
  AATGCAATCTTTATTGTGGAATTGCCAACATACCGTGTGCCTTCCATTAAAACGTTGTGG
  CGTAGTACTTGGGAAAAGGCTAAGGGCTTTGTTCGTAAAGCAGGTACATTTATTTTTGGC
  GGCTCAGTAGTCATTTGGCTATTAAGTTATGTAGGACCACATGGTATCAATGTGAATATC
  AATCAAAGTTTTTTACATATGGTTGGTAGTTTCTTTGGTATGTTAGTGCAACCGCTCGGA
  TTTGGAACATGGCAAGCAGGTGCTACGTTAGTGCCAGGATTTTTAGCAAAAGAAGTTATC
  GTAAGTTCGATGGCGATTATCTATTCATCAGGTGATGCAGGCCTTGTTAATGTGATTCAA
  AATCAATTCACACCGCTATCTGCGTATGCATTTATGATATTTATACTATTGTATATTCCT
  TGTGTTTCTACAGTAGCAGCAATCCGTAAAGAGACTTATTCATGGAAATGGACGGCACTT
  GCAGTTGCCTATCCTCTAGTCACTGCATATGTCTTAACATTCATTTTTTATCAAGTCGGA
  CATTTATTTGTTTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1500
  1560
  1620
  1680
  1740
  1800
  1860
  1920
  1980
  1995
  

⊟Protein

⊟General

• locus tag: NWMN_RS14080 [old locus tag: NWMN_2450 ]
• symbol: NWMN_RS14080
• description: ferrous iron transporter B
• length: 664
• theoretical pI: 8.54816
• theoretical MW: 74384.8
• GRAVY: 0.367319

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Cations and iron carrying compounds ferrous iron transport protein B (TIGR00437; HMM-score: 624.7)
  and 10 more
  Unknown function General small GTP-binding protein domain (TIGR00231; HMM-score: 42.2)
  Genetic information processing Protein synthesis Other ribosome-associated GTPase EngA (TIGR03594; HMM-score: 35.4)
  Genetic information processing Protein synthesis Other ribosome biogenesis GTP-binding protein YlqF (TIGR03596; HMM-score: 27.6)
  Genetic information processing Protein synthesis Other ribosome biogenesis GTP-binding protein YsxC (TIGR03598; HMM-score: 23.5)
  Genetic information processing Protein fate Protein modification and repair [FeFe] hydrogenase H-cluster maturation GTPase HydF (TIGR03918; HMM-score: 21.3)
  Genetic information processing Protein synthesis Other GTP-binding protein Era (TIGR00436; HMM-score: 21.1)
  Genetic information processing Protein synthesis Other Obg family GTPase CgtA (TIGR02729; HMM-score: 17.8)
  Unknown function General GTP-binding protein YchF (TIGR00092; HMM-score: 15.4)
  Unknown function General GTP-binding protein HflX (TIGR03156; HMM-score: 11.5)
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA modification GTPase TrmE (TIGR00450; EC 3.6.-.-; HMM-score: 10.3)
• TheSEED: see NWMN_2450
• PFAM:
  P-loop_NTPase (CL0023) FeoB_N; Ferrous iron transport protein B (PF02421; HMM-score: 194.2)
  and 8 more
  no clan defined Gate; Nucleoside recognition (PF07670; HMM-score: 147.6)
  FeoB_C; Ferrous iron transport protein B C terminus (PF07664; HMM-score: 69.2)
  P-loop_NTPase (CL0023) MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 59)
  no clan defined FeoB_Cyto; FeoB cytosolic helical domain (PF17910; HMM-score: 46.3)
  P-loop_NTPase (CL0023) SRPRB; Signal recognition particle receptor beta subunit (PF09439; HMM-score: 22.1)
  ATP_bind_1; Conserved hypothetical ATP binding protein (PF03029; HMM-score: 14.7)
  no clan defined WipA_N; WipA, alpha-helical hairpin (PF21662; HMM-score: 13.8)
  P-loop_NTPase (CL0023) Dynamin_N; Dynamin family (PF00350; HMM-score: 11.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 9
• DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.0001
  • Cytoplasmic Membrane Score: 0.9994
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0006
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.023195
  • TAT(Tat/SPI): 0.000284
  • LIPO(Sec/SPII): 0.003767
• predicted transmembrane helices (TMHMM): 10

⊟Accession numbers

• GI: 446355052 NCBI
• RefSeq: WP_000432907 NCBI
• UniProt: see NWMN_2450

⊟Protein sequence

• MENYCILGNPNVGKTSLFNALTGSYEYIGNWSGVTVEKKVGKLKENVGQLIDLPGTYDLSPISKDETVVTDYLLNDSFSGIINIVDASQLKRNMQLTVQLLELNQPIYIGLNMIDVATKRGIKIDYHKLMKKLKTPIFPVVARTGKGTKYLLGEIKHLGEGYQPHFKINYGEKIEETIKNMCQIIMTETSHDKYQARFIAIQFLLNNMQIANELNSEVVNKLSSLRDQVAEQVGAVSVRREMERIRNHYIETLLQDVVTYPDEDKQYFSSRIDKILTHKYIGMPIFLAIMWLIFQTTFTWIGTPLSDQLDAFIGGTFTDSVKTIMNYLGVIPFLQDLITDGIIAGVGSVLVFVPQIVVLFFFISLLEDSGYMARIAVLMDRIMESFGLSGKSFIPMIIGFGCNVPSIMAARSIENEKERLTTILIAPFMSCSARLPVYALFVGIFFKENQSLVVLSLYVLGIIMAFLVSTVLTKTILKNDNAIFIVELPTYRVPSIKTLWRSTWEKAKGFVRKAGTFIFGGSVVIWLLSYVGPHGINVNINQSFLHMVGSFFGMLVQPLGFGTWQAGATLVPGFLAKEVIVSSMAIIYSSGDAGLVNVIQNQFTPLSAYAFMIFILLYIPCVSTVAAIRKETYSWKWTALAVAYPLVTAYVLTFIFYQVGHLFV

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: Fur*, SigB see NWMN_2450

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications