PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2487 [new locus tag: SAUSA300_RS13815 ]
• pan locus tag^?: SAUPAN006212000
• symbol: feoB
• pan gene symbol^?: feoB
• synonym:
• product: ferrous iron transport protein B

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2487 [new locus tag: SAUSA300_RS13815 ]
• symbol: feoB
• product: ferrous iron transport protein B
• replicon: chromosome
• strand: -
• coordinates: 2687552..2689546
• length: 1995
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3915251 NCBI
• RefSeq: YP_495121 NCBI
• BioCyc: see SAUSA300_RS13815
• MicrobesOnline: 1294002 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGGAAAATTATTGTATTTTAGGAAATCCTAATGTTGGTAAAACATCTTTGTTTAACGCT
  TTAACTGGTTCGTATGAATATATAGGCAACTGGAGTGGCGTAACGGTAGAGAAAAAAGTA
  GGGAAACTTAAAGAAAATGTAGGACAATTAATTGATTTACCAGGTACATATGATTTATCG
  CCGATTTCTAAAGATGAAACTGTAGTGACAGACTATTTATTAAACGATTCATTTTCAGGA
  ATCATTAACATTGTTGATGCGAGTCAACTAAAAAGAAATATGCAATTAACAGTACAATTG
  TTAGAACTTAATCAACCAATATACATCGGATTAAATATGATTGATGTAGCTACAAAGCGC
  GGGATAAAAATCGATTATCATAAATTGATGAAAAAGTTAAAAACACCTATTTTTCCAGTT
  GTGGCACGTACAGGCAAAGGAACAAAATATTTACTTGGCGAAATAAAGCATCTAGGGGAA
  GGGTATCAACCGCATTTTAAAATCAATTATGGTGAGAAGATTGAAGAGACAATTAAAAAT
  ATGTGTCAAATAATAATGACAGAGACATCTCATGATAAGTATCAAGCTAGGTTTATTGCG
  ATTCAATTCTTATTAAATAACATGCAAATTGCAAACGAACTTAATTCAGAGGTTGTAAAT
  AAATTGTCGAGTTTACGTGATCAAGTTGCTGAACAAGTAGGGGCTGTATCTGTTCGTCGA
  GAGATGGAGCGTATACGCAATCATTATATTGAGACGTTATTGCAAGACGTGGTAACGTAT
  CCAGATGAAGATAAGCAATATTTCAGCTCTAGAATAGACAAGATATTAACACATAAATAT
  ATAGGGATGCCTATCTTTCTAGCAATTATGTGGTTGATCTTTCAAACTACATTTACATGG
  ATTGGCACACCTTTGTCGGATCAACTCGATGCATTTATAGGTGGTACATTTACAGATAGT
  GTGAAGACGATTATGAATTATCTTGGTGTTATACCATTTTTACAAGATTTAATTACAGAT
  GGCATTATTGCTGGTGTAGGATCAGTATTAGTCTTTGTACCACAAATTGTTGTGCTCTTT
  TTCTTTATATCTTTATTAGAAGATTCGGGTTATATGGCACGTATTGCGGTTTTAATGGAT
  CGTATTATGGAATCGTTCGGTTTAAGCGGGAAGTCTTTTATACCAATGATTATCGGATTT
  GGTTGTAATGTACCGAGTATTATGGCTGCGCGCAGTATTGAAAATGAAAAAGAGCGTTTA
  ACAACGATATTAATTGCACCATTCATGTCATGCTCTGCTAGACTGCCAGTGTATGCGTTG
  TTCGTAGGTATCTTTTTCAAAGAGAATCAATCTTTAGTAGTATTAAGCCTGTATGTTTTA
  GGTATTATTATGGCATTTTTAGTCAGTACAGTACTTACTAAGACGATTTTAAAAAATGAT
  AATGCAATCTTTATTGTGGAATTGCCAACATACCGTGTGCCTTCCATTAAAACGTTGTGG
  CGTAGTACTTGGGAAAAGGCTAAGGGCTTTGTTCGTAAAGCAGGTACATTTATTTTTGGC
  GGCTCAGTAGTCATTTGGCTATTAAGTTATGTAGGACCACATGGTATCAATGTGAATATC
  AATCAAAGTTTTTTACATATGGTTGGTAGTTTCTTTGGTATGTTAGTGCAACCGCTCGGA
  TTTGGAACATGGCAAGCAGGTGCTACGTTAGTGCCAGGATTTTTAGCAAAAGAAGTTATC
  GTAAGTTCGATGGCGATTATCTATTCATCAGGTGATGCAGGCCTTGTTAATGTGATTCAA
  AATCAATTCACACCGCTATCTGCGTATGCATTTATGATATTTATACTATTGTATATTCCT
  TGTGTTTCTACAGTAGCAGCAATCCGTAAAGAGACTTATTCATGGAAATGGACGGCACTT
  GCAGTTGCCTATCCTCTAGTCACTGCATATGTCTTAACATTCATTTTTTATCAAGTCGGA
  CATTTATTTGTTTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_2487 [new locus tag: SAUSA300_RS13815 ]
• symbol: FeoB
• description: ferrous iron transport protein B
• length: 664
• theoretical pI: 8.54816
• theoretical MW: 74384.8
• GRAVY: 0.367319

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Cations and iron carrying compounds ferrous iron transport protein B (TIGR00437; HMM-score: 624.7)
  and 10 more
  Unknown function General small GTP-binding protein domain (TIGR00231; HMM-score: 42.2)
  Genetic information processing Protein synthesis Other ribosome-associated GTPase EngA (TIGR03594; HMM-score: 35.4)
  Genetic information processing Protein synthesis Other ribosome biogenesis GTP-binding protein YlqF (TIGR03596; HMM-score: 27.6)
  Genetic information processing Protein synthesis Other ribosome biogenesis GTP-binding protein YsxC (TIGR03598; HMM-score: 23.5)
  Genetic information processing Protein fate Protein modification and repair [FeFe] hydrogenase H-cluster maturation GTPase HydF (TIGR03918; HMM-score: 21.3)
  Genetic information processing Protein synthesis Other GTP-binding protein Era (TIGR00436; HMM-score: 21.1)
  Genetic information processing Protein synthesis Other Obg family GTPase CgtA (TIGR02729; HMM-score: 17.8)
  Unknown function General GTP-binding protein YchF (TIGR00092; HMM-score: 15.4)
  Unknown function General GTP-binding protein HflX (TIGR03156; HMM-score: 11.5)
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA modification GTPase TrmE (TIGR00450; EC 3.6.-.-; HMM-score: 10.3)
• TheSEED  :
  • Ferrous iron transport protein B
  CBSS-196620.1.peg.2477  Ferrous iron transport protein B
  and 1 more
  RCJ pfr  Ferrous iron transport protein B
• PFAM:
  P-loop_NTPase (CL0023) FeoB_N; Ferrous iron transport protein B (PF02421; HMM-score: 191.9)
  and 6 more
  no clan defined Gate; Nucleoside recognition (PF07670; HMM-score: 152.3)
  FeoB_C; Ferrous iron transport protein B C terminus (PF07664; HMM-score: 67.9)
  P-loop_NTPase (CL0023) MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 56.1)
  SRPRB; Signal recognition particle receptor beta subunit (PF09439; HMM-score: 21.7)
  ATP_bind_1; Conserved hypothetical ATP binding protein (PF03029; HMM-score: 13.2)
  Peptidase_CA (CL0125) Transglut_core2; Transglutaminase-like superfamily (PF13369; HMM-score: 11.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 9
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.023195
  • TAT(Tat/SPI): 0.000284
  • LIPO(Sec/SPII): 0.003767
• predicted transmembrane helices (TMHMM): 10

⊟Accession numbers

• GI: 87161280 NCBI
• RefSeq: YP_495121 NCBI
• UniProt: A0A0H2XI90 UniProt

⊟Protein sequence

• MENYCILGNPNVGKTSLFNALTGSYEYIGNWSGVTVEKKVGKLKENVGQLIDLPGTYDLSPISKDETVVTDYLLNDSFSGIINIVDASQLKRNMQLTVQLLELNQPIYIGLNMIDVATKRGIKIDYHKLMKKLKTPIFPVVARTGKGTKYLLGEIKHLGEGYQPHFKINYGEKIEETIKNMCQIIMTETSHDKYQARFIAIQFLLNNMQIANELNSEVVNKLSSLRDQVAEQVGAVSVRREMERIRNHYIETLLQDVVTYPDEDKQYFSSRIDKILTHKYIGMPIFLAIMWLIFQTTFTWIGTPLSDQLDAFIGGTFTDSVKTIMNYLGVIPFLQDLITDGIIAGVGSVLVFVPQIVVLFFFISLLEDSGYMARIAVLMDRIMESFGLSGKSFIPMIIGFGCNVPSIMAARSIENEKERLTTILIAPFMSCSARLPVYALFVGIFFKENQSLVVLSLYVLGIIMAFLVSTVLTKTILKNDNAIFIVELPTYRVPSIKTLWRSTWEKAKGFVRKAGTFIFGGSVVIWLLSYVGPHGINVNINQSFLHMVGSFFGMLVQPLGFGTWQAGATLVPGFLAKEVIVSSMAIIYSSGDAGLVNVIQNQFTPLSAYAFMIFILLYIPCVSTVAAIRKETYSWKWTALAVAYPLVTAYVLTFIFYQVGHLFV

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: feoB < feoA

⊟Regulation

• regulator: Fur* (repression) regulon

  Fur*

  (TF)

  important in Iron homeostasis; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications