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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

organism: Staphylococcus aureus Newman
locus tag: NWMN_RS14020 [old locus tag: NWMN_2440 ]
pan locus tag^?: SAUPAN006198000
symbol: NWMN_RS14020
pan gene symbol^?: cidA
synonym:
product: holin-like protein CidA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

type: CDS
locus tag: NWMN_RS14020 [old locus tag: NWMN_2440 ]
symbol: NWMN_RS14020
product: holin-like protein CidA
replicon: chromosome
strand: -
coordinates: 2683152..2683547
length: 396
essential: unknown other strains

⊟Accession numbers[edit | edit source]

Location: NC_009641 (2683152..2683547) NCBI
BioCyc:
MicrobesOnline: see NWMN_2440

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

1
61
121
181
241
301
361
ATGCACAAAGTCCAATTAATAATCAAACTACTACTACAACTAGGAATCATCATTGTGATT
ACTTATATTGGCACAGAAATTCAAAAGATTTTTCATCTTCCCTTAGCCGGCAGTATTGTT
GGTCTATTTTTATTTTATTTACTATTACAATTTAAGATTGTACCGCTAACTTGGGTAGAA
GACGGTGCAAACTTTTTATTAAAGACGATGGTCTTTTTCTTCATACCGTCAGTTGTAGGT
ATTATGGATGTTGCTTCCGAAATTACGCTAAATTATATACTCTTTTTCGCAGTCATTATC
ATAGGAACATGTATCGTTGCATTATCTTCAGGTTATATTGCTGAAAAAATGTCTGTTAAA
CATAAACATCGTAAAGGTGTAGACGCTTATGAATGA
60
120
180
240
300
360
396

⊟Protein[edit | edit source]

⊟General[edit | edit source]

locus tag: NWMN_RS14020 [old locus tag: NWMN_2440 ]
symbol: NWMN_RS14020
description: holin-like protein CidA
length: 131
theoretical pI: 9.01426
theoretical MW: 14729.8
GRAVY: 1.04962

⊟Function[edit | edit source]

TIGRFAM:
TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
PFAM:
no clan defined LrgA; LrgA family (PF03788; HMM-score: 83.4)

⊟Structure, modifications & cofactors[edit | edit source]

domains:
modifications:
cofactors:
effectors:

⊟Localization[edit | edit source]

PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 4
LocateP:
SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.004866
- TAT(Tat/SPI): 0.000114
- LIPO(Sec/SPII): 0.016401
predicted transmembrane helices (TMHMM): 4

⊟Accession numbers[edit | edit source]

GI: 446471880 NCBI
RefSeq: WP_000549734 NCBI
UniProt: see NWMN_2440

⊟Protein sequence[edit | edit source]

MHKVQLIIKLLLQLGIIIVITYIGTEIQKIFHLPLAGSIVGLFLFYLLLQFKIVPLTWVEDGANFLLKTMVFFFIPSVVGIMDVASEITLNYILFFAVIIIGTCIVALSSGYIAEKMSVKHKHRKGVDAYE

⊟Experimental data[edit | edit source]

experimentally validated:
protein localization:
quantitative data / protein copy number per cell:
interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

regulator: SigB see NWMN_2440

⊟Transcription pattern[edit | edit source]

S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

Aureolib: no data available

⊟Protein stability[edit | edit source]

half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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