PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS13305 [old locus tag: NWMN_2314 ]
• pan locus tag^?: SAUPAN005949000
• symbol: NWMN_RS13305
• pan gene symbol^?: mdeA
• synonym:
• product: MFS transporter

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS13305 [old locus tag: NWMN_2314 ]
• symbol: NWMN_RS13305
• product: MFS transporter
• replicon: chromosome
• strand: -
• coordinates: 2540713..2542071
• length: 1359
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (2540713..2542071) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_2314

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  GTGACGGCTTACCCAAGTATTATGAAAGATTTTGAAATTTCATATACACAAGTTCAATGG
  CTGACAACAGCTTTTTTATTGACTAATGGGATTGTTATTCCTTTGTCCGCGCTTGTTATA
  CAACGTTATACAACAAGACAAGTGTTTTTAGTGGGTATTTCTATCTTTTTCTTAGGTACA
  TTACTCGGCGGCTTGAGTCCGCACTTTGCAACATTATTAGTTGCTAGAATTATTCAGGCG
  TTAGGCGCAGGTATTATGATGCCATTGATGATGACAACGATTTTGGATGTTTTCCAACCA
  CATGAACGCGGTAAATATATGGGGATATTTGGTTTGGTAATTGGTTTAGCACCAGCTATT
  GGACCTACTCTTTCAGGTTACCTTGTTGAATATTTTAACTGGAGATCGCTTTTCCATGTT
  GTCGCTCCAATTGCAGCTGTGACATTTTTAATTGGATTTAAAACGATAAAAAATGTTGGA
  ACTACAATTAAAGTACCTATTGATTTTATTTCTGTCATTTTTTCTGTACTAGGTTTCGGC
  GGGTTATTGTATGGAACGAGTTCAATTTCAGAAAAAGGTTTTGATAATCCTATCGTATTA
  GTATCTATGATTGGAGGCGTTGTTTTAGTCGCATTATTTGTATTACGTCAATATCGGCTA
  TCAACACCATTATTAAATTTTGCTGTATTTAAAAATAAACAATTTACAGTTGGTATCATT
  ATTATGGGTGTCACAATGGTATCGATGATTGGTTCGGAAACGATTTTACCTATCTTTGTG
  CAAAATTTATTGCATCGTTCAGCTTTAGATTCTGGATTAACTTTATTACCAGGAGCAATT
  GTTATGGCATTTATGTCGATGACTTCGGGTGCTTTATATGAAAAGTTTGGTCCTAGAAAT
  CTTGCTTTAGTAGGTATGGCGATTGTTGTTATTACTACGGCTTATTTTGTTGTAATGGAT
  GAACAAACATCAACAATCATGTTGGCAACAGTTTATGCGATTCGAATGGTTGGTATCGCG
  TTAGGATTAATTCCAGTAATGACCCATACGATGAATCAATTAAAGCCAGAAATGAATGCA
  CATGGTTCATCTATGACAAACACAGTACAACAAATTGCTGGTTCTATCGGAACAGCTGCA
  TTAATTACTATTTTATCCCATGCAAGCAAAAACTTTTCTCCAACTATGTCAGACTATAAT
  GGCATGAACAAAATTGATATGATGAATCAAATTAAAGTTGATACGATGTTGCATGGTTAT
  CACGCTGGATTTTTATTTGCATTATTGATTACAGTTGTTAGTTTCTTTTGTTCATTTATG
  TTACAAGGTAAGAAAAAAGAAGTTGATTCTCGTCAATAA
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⊟Protein

⊟General

• locus tag: NWMN_RS13305 [old locus tag: NWMN_2314 ]
• symbol: NWMN_RS13305
• description: MFS transporter
• length: 452
• theoretical pI: 9.82904
• theoretical MW: 49228.5
• GRAVY: 0.742035

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 327.8)
  Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 327.8)
  and 16 more
  drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 89.4)
  multidrug resistance protein (TIGR00880; HMM-score: 81.3)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 48.9)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 46.9)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 45.9)
  Metabolism Transport and binding proteins Unknown substrate MFS transporter, metabolite:H+ symporter (MHS) family protein (TIGR00883; HMM-score: 34.6)
  oxalate/formate antiporter (TIGR04259; HMM-score: 26.1)
  Metabolism Transport and binding proteins Cations and iron carrying compounds H+ Antiporter protein (TIGR00900; HMM-score: 25.8)
  Metabolism Transport and binding proteins Anions Na(+)-dependent inorganic phosphate cotransporter (TIGR00894; HMM-score: 25.3)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids monocarboxylate transporter (TIGR00892; HMM-score: 23.6)
  Metabolism Transport and binding proteins Anions nitrite transporter (TIGR00886; HMM-score: 22)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, sialate:H+ symporter (SHS) family (TIGR00891; HMM-score: 18.1)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids oxalate/formate antiporter family transporter (TIGR00890; HMM-score: 16.8)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids polyol permease (TIGR00897; HMM-score: 13.6)
  sodium-independent organic anion transporter (TIGR00805; HMM-score: 6.3)
  synaptic vesicle protein SV2 (TIGR01299; HMM-score: 4.3)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 175.2)
  and 2 more
  MFS_2; MFS/sugar transport protein (PF13347; HMM-score: 32.4)
  OATP; Organic Anion Transporter Polypeptide (OATP) family (PF03137; HMM-score: 9.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 13
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001847
  • TAT(Tat/SPI): 0.000151
  • LIPO(Sec/SPII): 0.002913
• predicted transmembrane helices (TMHMM): 13

⊟Accession numbers

• GI: 446050238 NCBI
• RefSeq: WP_000128093 NCBI
• UniProt: see NWMN_2314

⊟Protein sequence

• MTAYPSIMKDFEISYTQVQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGTLLGGLSPHFATLLVARIIQALGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYFNWRSLFHVVAPIAAVTFLIGFKTIKNVGTTIKVPIDFISVIFSVLGFGGLLYGTSSISEKGFDNPIVLVSMIGGVVLVALFVLRQYRLSTPLLNFAVFKNKQFTVGIIIMGVTMVSMIGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYEKFGPRNLALVGMAIVVITTAYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSMTNTVQQIAGSIGTAALITILSHASKNFSPTMSDYNGMNKIDMMNQIKVDTMLHGYHAGFLFALLITVVSFFCSFMLQGKKKEVDSRQ

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: Zur* see NWMN_2314

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications