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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS13305 [old locus tag: NWMN_2314 ]
  • pan locus tag?: SAUPAN005949000
  • symbol: NWMN_RS13305
  • pan gene symbol?: mdeA
  • synonym:
  • product: MFS transporter

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS13305 [old locus tag: NWMN_2314 ]
  • symbol: NWMN_RS13305
  • product: MFS transporter
  • replicon: chromosome
  • strand: -
  • coordinates: 2540713..2542071
  • length: 1359
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (2540713..2542071) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_2314

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    GTGACGGCTTACCCAAGTATTATGAAAGATTTTGAAATTTCATATACACAAGTTCAATGG
    CTGACAACAGCTTTTTTATTGACTAATGGGATTGTTATTCCTTTGTCCGCGCTTGTTATA
    CAACGTTATACAACAAGACAAGTGTTTTTAGTGGGTATTTCTATCTTTTTCTTAGGTACA
    TTACTCGGCGGCTTGAGTCCGCACTTTGCAACATTATTAGTTGCTAGAATTATTCAGGCG
    TTAGGCGCAGGTATTATGATGCCATTGATGATGACAACGATTTTGGATGTTTTCCAACCA
    CATGAACGCGGTAAATATATGGGGATATTTGGTTTGGTAATTGGTTTAGCACCAGCTATT
    GGACCTACTCTTTCAGGTTACCTTGTTGAATATTTTAACTGGAGATCGCTTTTCCATGTT
    GTCGCTCCAATTGCAGCTGTGACATTTTTAATTGGATTTAAAACGATAAAAAATGTTGGA
    ACTACAATTAAAGTACCTATTGATTTTATTTCTGTCATTTTTTCTGTACTAGGTTTCGGC
    GGGTTATTGTATGGAACGAGTTCAATTTCAGAAAAAGGTTTTGATAATCCTATCGTATTA
    GTATCTATGATTGGAGGCGTTGTTTTAGTCGCATTATTTGTATTACGTCAATATCGGCTA
    TCAACACCATTATTAAATTTTGCTGTATTTAAAAATAAACAATTTACAGTTGGTATCATT
    ATTATGGGTGTCACAATGGTATCGATGATTGGTTCGGAAACGATTTTACCTATCTTTGTG
    CAAAATTTATTGCATCGTTCAGCTTTAGATTCTGGATTAACTTTATTACCAGGAGCAATT
    GTTATGGCATTTATGTCGATGACTTCGGGTGCTTTATATGAAAAGTTTGGTCCTAGAAAT
    CTTGCTTTAGTAGGTATGGCGATTGTTGTTATTACTACGGCTTATTTTGTTGTAATGGAT
    GAACAAACATCAACAATCATGTTGGCAACAGTTTATGCGATTCGAATGGTTGGTATCGCG
    TTAGGATTAATTCCAGTAATGACCCATACGATGAATCAATTAAAGCCAGAAATGAATGCA
    CATGGTTCATCTATGACAAACACAGTACAACAAATTGCTGGTTCTATCGGAACAGCTGCA
    TTAATTACTATTTTATCCCATGCAAGCAAAAACTTTTCTCCAACTATGTCAGACTATAAT
    GGCATGAACAAAATTGATATGATGAATCAAATTAAAGTTGATACGATGTTGCATGGTTAT
    CACGCTGGATTTTTATTTGCATTATTGATTACAGTTGTTAGTTTCTTTTGTTCATTTATG
    TTACAAGGTAAGAAAAAAGAAGTTGATTCTCGTCAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1359

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS13305 [old locus tag: NWMN_2314 ]
  • symbol: NWMN_RS13305
  • description: MFS transporter
  • length: 452
  • theoretical pI: 9.82904
  • theoretical MW: 49228.5
  • GRAVY: 0.742035

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 327.8)
    Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 327.8)
    and 16 more
    drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 89.4)
    multidrug resistance protein (TIGR00880; HMM-score: 81.3)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 48.9)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 46.9)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 45.9)
    Metabolism Transport and binding proteins Unknown substrate MFS transporter, metabolite:H+ symporter (MHS) family protein (TIGR00883; HMM-score: 34.6)
    oxalate/formate antiporter (TIGR04259; HMM-score: 26.1)
    Metabolism Transport and binding proteins Cations and iron carrying compounds H+ Antiporter protein (TIGR00900; HMM-score: 25.8)
    Metabolism Transport and binding proteins Anions Na(+)-dependent inorganic phosphate cotransporter (TIGR00894; HMM-score: 25.3)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids monocarboxylate transporter (TIGR00892; HMM-score: 23.6)
    Metabolism Transport and binding proteins Anions nitrite transporter (TIGR00886; HMM-score: 22)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, sialate:H+ symporter (SHS) family (TIGR00891; HMM-score: 18.1)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids oxalate/formate antiporter family transporter (TIGR00890; HMM-score: 16.8)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids polyol permease (TIGR00897; HMM-score: 13.6)
    sodium-independent organic anion transporter (TIGR00805; HMM-score: 6.3)
    synaptic vesicle protein SV2 (TIGR01299; HMM-score: 4.3)
  • TheSEED: see NWMN_2314
  • ⊞PFAM:
    MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 190.4)
    and 2 more
    MFS_2; MFS/sugar transport protein (PF13347; HMM-score: 35.8)
    OATP; Organic Anion Transporter Polypeptide (OATP) family (PF03137; HMM-score: 15.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 13
  • ⊞DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 1
    • Cell wall & surface Score: 0
    • Extracellular Score: 0
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001847
    • TAT(Tat/SPI): 0.000151
    • LIPO(Sec/SPII): 0.002913
  • predicted transmembrane helices (TMHMM): 13

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTAYPSIMKDFEISYTQVQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGTLLGGLSPHFATLLVARIIQALGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYFNWRSLFHVVAPIAAVTFLIGFKTIKNVGTTIKVPIDFISVIFSVLGFGGLLYGTSSISEKGFDNPIVLVSMIGGVVLVALFVLRQYRLSTPLLNFAVFKNKQFTVGIIIMGVTMVSMIGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYEKFGPRNLALVGMAIVVITTAYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSMTNTVQQIAGSIGTAALITILSHASKNFSPTMSDYNGMNKIDMMNQIKVDTMLHGYHAGFLFALLITVVSFFCSFMLQGKKKEVDSRQ

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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