PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS11620 [old locus tag: NWMN_2009 ]
• pan locus tag^?: SAUPAN005396000
• symbol: NWMN_RS11620
• pan gene symbol^?: atpA
• synonym:
• product: ATP synthase subunit alpha

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS11620 [old locus tag: NWMN_2009 ]
• symbol: NWMN_RS11620
• product: ATP synthase subunit alpha
• replicon: chromosome
• strand: -
• coordinates: 2227853..2229361
• length: 1509
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (2227853..2229361) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_2009

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  1021
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  1321
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  1441
  1501
  
  ATGGCCATAAAAGCTGAAGAAATCAGTGCATTACTTCGCTCACAAATTGAAAATTATGAG
  TCAGAAATGTCCGTAACTGATGTAGGTACTGTATTACAAATTGGTGATGGTATTGCATTA
  ATTCACGGATTAAATGACGTTATGGCTGGTGAGCTAGTAGAATTCCATAACGGCGTACTT
  GGTTTAGCCCAAAACCTTGAAGAGTCAAACGTGGGTGTGGTTATTTTAGGACCATACACA
  GGTATTACTGAAGGTGACGAAGTTAAACGTACTGGTCGTATCATGGAAGTACCAGTAGGT
  GAAGAACTAATCGGAAGAGTTGTTAATCCATTAGGACAACCTATTGATGGACAAGGACCG
  ATTAACACAACTAAAACACGTCCAGTAGAGAAAAAAGCTACTGGTGTAATGGATCGTAAA
  TCAGTAGATGAGCCATTACAAACAGGTATCAAAGCAATTGATGCTTTAGTACCAATTGGT
  AGAGGTCAACGTGAGTTAATCATCGGTGACCGTCAAACAGGTAAAACAACAATTGCAATT
  GACACAATTTTGAACCAAAAAGATCAAGGTACGATTTGTATCTATGTTGCTATTGGTCAA
  AAAGATTCAACAGTAAGAGCAAATGTTGAAAAGTTAAGACAAGCAGGCGCTTTAGACTAC
  ACTATTGTTGTAGCAGCATCAGCTTCTGAACCTTCTCCATTATTATATATTGCACCATAT
  TCAGGTGTAACAATGGGTGAAGAATTCATGTTTAACGGTAAACATGTTTTAATCGTTTAT
  GATGATTTAACTAAACAAGCAGCAGCTTATCGTGAGTTATCATTATTATTACGTAGACCT
  CCAGGTCGTGAAGCATACCCAGGTGACGTATTCTACTTACATAGTAGATTATTAGAAAGA
  GCAGCAAAATTAAACGATGACTTAGGTGGCGGTTCAATTACTGCATTACCAATTATCGAA
  ACACAAGCTGGTGATATTTCAGCTTATGTACCAACAAACGTTATTTCAATTACAGATGGA
  CAAATCTTCTTACAATCTGATTTATTCTTCTCAGGTGTAAGACCAGCGATTAATGCCGGA
  CAATCTGTATCTCGTGTTGGTGGATCTGCACAAATTAATGCAATGAAGAAAGTTGCTGGT
  ACGTTACGTCTTGACTTAGCGTCATACAGAGAACTTGAATCATTTGCACAATTCGGTTCA
  GACCTTGATGAATTTACTGCAAGTAAATTAGAACGTGGTAAACGTACTGTTGAAGTCTTA
  AAACAAGATCAAAACAAACCATTACCAGTCGAACACCAAGTGTTGATTATTTATGCATTA
  ACAAAAGGATATTTAGATGATATTCCAGTTGTAGATATCACACGTTTTGAAGACGAGTTA
  AACCACTGGGCAGAATCAAATGCTACTGAACTGTTAAATGAAATCAGAGAAACTGGTGGC
  TTACCAGATGCTGAGAAGTTTGACACAGCAATTAACGAATTCAAAAAAAGCTTTAGCAAA
  TCTGAATAA
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  1509
  

⊟Protein

⊟General

• locus tag: NWMN_RS11620 [old locus tag: NWMN_2009 ]
• symbol: NWMN_RS11620
• description: ATP synthase subunit alpha
• length: 502
• theoretical pI: 4.59198
• theoretical MW: 54569.6
• GRAVY: -0.147012

⊟Function

• reaction:
  EC 3.6.3.14?  ExPASy
  H⁺-transporting two-sector ATPase ATP + H₂O + H⁺(In) = ADP + phosphate + H⁺(Out)
• TIGRFAM:
  Metabolism Energy metabolism ATP-proton motive force interconversion ATP synthase F1, alpha subunit (TIGR00962; EC 3.6.3.14; HMM-score: 833.8)
  and 17 more
  alternate F1F0 ATPase, F1 subunit alpha (TIGR03324; EC 3.6.3.-; HMM-score: 649.1)
  Cellular processes Cellular processes Pathogenesis type III secretion apparatus H+-transporting two-sector ATPase (TIGR02546; EC 3.6.3.14; HMM-score: 205.2)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking type III secretion apparatus H+-transporting two-sector ATPase (TIGR02546; EC 3.6.3.14; HMM-score: 205.2)
  Cellular processes Cellular processes Chemotaxis and motility flagellar protein export ATPase FliI (TIGR03496; EC 3.6.3.14; HMM-score: 198.8)
  Metabolism Energy metabolism ATP-proton motive force interconversion ATPase, FliI/YscN family (TIGR01026; EC 3.6.3.14; HMM-score: 187.2)
  Cellular processes Cellular processes Chemotaxis and motility flagellar protein export ATPase FliI (TIGR03497; EC 3.6.3.14; HMM-score: 183.1)
  flagellar protein export ATPase FliI (TIGR03498; EC 3.6.3.14; HMM-score: 173.5)
  Metabolism Energy metabolism ATP-proton motive force interconversion ATP synthase archaeal, B subunit (TIGR01041; EC 3.6.3.14; HMM-score: 147.6)
  Metabolism Energy metabolism ATP-proton motive force interconversion ATP synthase F1, beta subunit (TIGR01039; EC 3.6.3.14; HMM-score: 130.2)
  alternate F1F0 ATPase, F1 subunit beta (TIGR03305; EC 3.6.3.-; HMM-score: 121.4)
  Metabolism Transport and binding proteins Cations and iron carrying compounds V-type ATPase, B subunit (TIGR01040; EC 3.6.3.14; HMM-score: 118.9)
  Metabolism Energy metabolism ATP-proton motive force interconversion ATP synthase archaeal, A subunit (TIGR01043; EC 3.6.3.14; HMM-score: 69.7)
  Metabolism Transport and binding proteins Cations and iron carrying compounds V-type ATPase, A subunit (TIGR01042; EC 3.6.3.14; HMM-score: 51.9)
  Genetic information processing Transcription Transcription factors transcription termination factor Rho (TIGR00767; EC 3.6.4.-; HMM-score: 19.5)
  KaiC domain protein, AF_0351 family (TIGR03880; HMM-score: 16.3)
  KaiC domain protein, PAE1156 family (TIGR03881; HMM-score: 15.3)
  KaiC domain protein, Ph0284 family (TIGR03877; HMM-score: 13)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  P-loop_NTPase (CL0023) ATP-synt_ab; ATP synthase alpha/beta family, nucleotide-binding domain (PF00006; HMM-score: 246.9)
  and 7 more
  no clan defined ATP-synt_ab_C; ATP synthase alpha/beta chain, C terminal domain (PF00306; HMM-score: 151.2)
  HAS-barrel (CL0275) ATP-synt_ab_N; ATP synthase alpha/beta family, beta-barrel domain (PF02874; HMM-score: 67.1)
  P-loop_NTPase (CL0023) ATPase; KaiC (PF06745; HMM-score: 27.1)
  HAS-barrel (CL0275) HAS-barrel; HAS barrel domain (PF09378; HMM-score: 21.7)
  P-loop_NTPase (CL0023) RecA; recA bacterial DNA recombination protein (PF00154; HMM-score: 13.1)
  AAA_14; AAA domain (PF13173; HMM-score: 12.9)
  AAA_25; AAA domain (PF13481; HMM-score: 11.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00179
  • TAT(Tat/SPI): 0.00044
  • LIPO(Sec/SPII): 0.000183
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446897626 NCBI
• RefSeq: WP_000974882 NCBI
• UniProt: see NWMN_2009

⊟Protein sequence

• MAIKAEEISALLRSQIENYESEMSVTDVGTVLQIGDGIALIHGLNDVMAGELVEFHNGVLGLAQNLEESNVGVVILGPYTGITEGDEVKRTGRIMEVPVGEELIGRVVNPLGQPIDGQGPINTTKTRPVEKKATGVMDRKSVDEPLQTGIKAIDALVPIGRGQRELIIGDRQTGKTTIAIDTILNQKDQGTICIYVAIGQKDSTVRANVEKLRQAGALDYTIVVAASASEPSPLLYIAPYSGVTMGEEFMFNGKHVLIVYDDLTKQAAAYRELSLLLRRPPGREAYPGDVFYLHSRLLERAAKLNDDLGGGSITALPIIETQAGDISAYVPTNVISITDGQIFLQSDLFFSGVRPAINAGQSVSRVGGSAQINAMKKVAGTLRLDLASYRELESFAQFGSDLDEFTASKLERGKRTVEVLKQDQNKPLPVEHQVLIIYALTKGYLDDIPVVDITRFEDELNHWAESNATELLNEIRETGGLPDAEKFDTAINEFKKSFSKSE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications