PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS10655 [old locus tag: NWMN_1854 ]
• pan locus tag^?: SAUPAN004951000
• symbol: NWMN_RS10655
• pan gene symbol^?: sdcS
• synonym:
• product: sodium-dependent dicarboxylate transporter SdcS

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS10655 [old locus tag: NWMN_1854 ]
• symbol: NWMN_RS10655
• product: sodium-dependent dicarboxylate transporter SdcS
• replicon: chromosome
• strand: -
• coordinates: 2066032..2067594
• length: 1563
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (2066032..2067594) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_1854

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
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  481
  541
  601
  661
  721
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  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  1561
  
  ATGGCTTATTTCAATCAACATCAATCAATGATATCGAAAAGGTATTTAACATTCTTTTCA
  AAATCAAAGAAAAAGAAACCGTTTAGTGCGGGACAACTTATTGGACTAATATTAGGTCCA
  TTACTTTTCCTATTAACATTATTATTCTTTCATCCACAAGACTTACCTTGGAAAGGCGTC
  TATGTTTTAGCGATTACTTTATGGATTGCGACTTGGTGGATTACTGAAGCAATTCCTATT
  GCAGCAACGAGCTTATTACCAATTGTGTTATTACCATTAGGTCATATACTTACACCAGAA
  CAAGTATCATCCGAATATGGCAATGATATTATCTTTTTGTTTTTAGGTGGATTTATTTTG
  GCAATTGCAATGGAAAGATGGAATTTACATACGAGAGTAGCGTTGACAATTATTAATTTG
  ATTGGTGCAAGCACTTCAAAAATATTACTTGGATTCATGGTGGCAACAGGATTCTTATCT
  ATGTTTGTATCGAACACTGCAGCTGTAATGATTATGATTCCGATTGGTTTAGCAATTATT
  AAGGAAGCACATGATTTACAAGAAGCCAATACGAATCAAACAAGTATTCAAAAGTTTGAA
  AAATCTCTAGTTTTAGCAATTGGCTATGCAGGTACGATTGGTGGCTTGGGTACATTAATC
  GGAACCCCGCCATTAATTATTTTAAAAGGACAATACATGCAACATTTTGGACATGAAATT
  AGTTTTGCTAAATGGATGATTGTAGGGATTCCAACGGTCATTGTTTTGTTAGGTATTACT
  TGGCTCTATTTAAGATATGTTGCGTTTAGACATGATTTGAAATATTTACCTGGTGGTCAG
  ACGTTAATTAAACAAAAGTTAGACGAGCTTGGCAAAATGAAGTATGAAGAAAAGGTAGTA
  CAAACTATCTTTGTACTTGCTAGCTTATTATGGATTACAAGAGAGTTTCTTCTGAAAAAA
  TGGGAAGTTACGTCATCTGTTGCAGATGGTACGATTGCTATTTTTATATCAATATTATTA
  TTTATTATTCCAGCTAAAAATACTGAAAAACATCGCCGTATCATTGACTGGGAAGTTGCA
  AAAGAGCTCCCTTGGGGTGTATTAATTTTATTTGGTGGCGGTTTAGCATTAGCGAAAGGT
  ATTTCTGAAAGTGGTTTAGCAAAATGGTTAGGCGAACAGTTGAAATCATTAAATGGTGTT
  AGTCCGATTCTTATTGTAATTGTCATAACAATCTTTGTCTTATTTTTAACTGAAGTGACA
  TCTAATACTGCAACTGCAACGATGATTTTACCGATTTTAGCAACGTTGTCTGTTGCTGTT
  GGAGTGCATCCATTACTACTTATGGCACCTGCAGCTATGGCGGCTAACTGTGCATACATG
  TTACCAGTAGGGACACCACCGAATGCAATTATCTTTGGTTCTGGTAAAATATCTATCAAA
  CAAATGGCATCAGTAGGATTCTGGGTAAACTTAATCAGTGCAATAATTATTATTTTAGTC
  GTGTATTATGTAATGCCTATAGTTTTAGGTATTGATATAAATCAACCACTGCCATTGAAA
  TAG
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  1140
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  1260
  1320
  1380
  1440
  1500
  1560
  1563
  

⊟Protein

⊟General

• locus tag: NWMN_RS10655 [old locus tag: NWMN_1854 ]
• symbol: NWMN_RS10655
• description: sodium-dependent dicarboxylate transporter SdcS
• length: 520
• theoretical pI: 9.94707
• theoretical MW: 57171.4
• GRAVY: 0.766731

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Cations and iron carrying compounds transporter, divalent anion:Na+ symporter (DASS) family (TIGR00785; HMM-score: 294.3)
  Metabolism Transport and binding proteins Anions transporter, divalent anion:Na+ symporter (DASS) family (TIGR00785; HMM-score: 294.3)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  IT (CL0182) Na_sulph_symp; Sodium:sulfate symporter transmembrane region (PF00939; HMM-score: 316.3)
  and 1 more
  CitMHS; Citrate transporter (PF03600; HMM-score: 164.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 15
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002163
  • TAT(Tat/SPI): 0.000336
  • LIPO(Sec/SPII): 0.020447
• predicted transmembrane helices (TMHMM): 14

⊟Accession numbers

• GI: 446245306 NCBI
• RefSeq: WP_000323161 NCBI
• UniProt: see NWMN_1854

⊟Protein sequence

• MAYFNQHQSMISKRYLTFFSKSKKKKPFSAGQLIGLILGPLLFLLTLLFFHPQDLPWKGVYVLAITLWIATWWITEAIPIAATSLLPIVLLPLGHILTPEQVSSEYGNDIIFLFLGGFILAIAMERWNLHTRVALTIINLIGASTSKILLGFMVATGFLSMFVSNTAAVMIMIPIGLAIIKEAHDLQEANTNQTSIQKFEKSLVLAIGYAGTIGGLGTLIGTPPLIILKGQYMQHFGHEISFAKWMIVGIPTVIVLLGITWLYLRYVAFRHDLKYLPGGQTLIKQKLDELGKMKYEEKVVQTIFVLASLLWITREFLLKKWEVTSSVADGTIAIFISILLFIIPAKNTEKHRRIIDWEVAKELPWGVLILFGGGLALAKGISESGLAKWLGEQLKSLNGVSPILIVIVITIFVLFLTEVTSNTATATMILPILATLSVAVGVHPLLLMAPAAMAANCAYMLPVGTPPNAIIFGSGKISIKQMASVGFWVNLISAIIIILVVYYVMPIVLGIDINQPLPLK

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CcpA see NWMN_1854

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications