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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_RS10105 [old locus tag: NWMN_1767 ]
- pan locus tag?: SAUPAN004860000
- symbol: NWMN_RS10105
- pan gene symbol?: —
- synonym:
- product: glutamine amidotransferase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_RS10105 [old locus tag: NWMN_1767 ]
- symbol: NWMN_RS10105
- product: glutamine amidotransferase
- replicon: chromosome
- strand: -
- coordinates: 1979053..1979568
- length: 516
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
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481ATGACTAAAAAAGTAGCAATTATTCTAGCAAACGAATTTGAAGATATAGAATATTCAAGC
CCTAAAGAGGCATTAGAGAATGCAGGCTTTAATACTGTAGTGATTGGAGATACTGCAAAT
AGTGAAGTTGTTGGTAAACACGGTGAAAAAGTTACTGTCGATGTAGGCATTGCAGAAGCT
AAACCAGAAGATTATGATGCATTATTAATTCCTGGAGGATTTTCACCAGATCATTTACGT
GGAGATACAGAAGGTCGATATGGCACATTTGCTAAATACTTTACTAAAAATGATGTACCA
ACATTTGCCATTTGTCATGGGCCACAAATACTAATAGATACAGACGATTTAAAAGGTCGT
ACGTTAACAGCAGTATTAAATGTACGCAAAGATTTATCAAATGCAGGCGCACATGTAGTT
GATGAGTCAGTAGTTGTAGACAACAATATTGTAACAAGTCGAGTACCAGACGATTTAGAT
GATTTTAATCGAGAAATCGTTAAACAATTACAATAG60
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516
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_RS10105 [old locus tag: NWMN_1767 ]
- symbol: NWMN_RS10105
- description: glutamine amidotransferase
- length: 171
- theoretical pI: 4.36059
- theoretical MW: 18631.7
- GRAVY: -0.27193
⊟Function[edit | edit source]
- TIGRFAM: Protein fate Degradation of proteins, peptides, and glycopeptides intracellular protease, PfpI family (TIGR01382; HMM-score: 156.9)and 4 moreUnknown function General DJ-1 family protein (TIGR01383; HMM-score: 62.4)Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylformylglycinamidine synthase I (TIGR01737; EC 6.3.5.3; HMM-score: 25.1)Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis CTP synthase (TIGR00337; EC 6.3.4.2; HMM-score: 20.8)Amino acid biosynthesis Histidine family imidazole glycerol phosphate synthase, glutamine amidotransferase subunit (TIGR01855; EC 2.4.2.-; HMM-score: 13.6)
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: Glutaminase_I (CL0014) DJ-1_PfpI; DJ-1/PfpI family (PF01965; HMM-score: 178.4)and 4 moreGATase; Glutamine amidotransferase class-I (PF00117; HMM-score: 25.6)GATase_5; CobB/CobQ-like glutamine amidotransferase domain (PF13507; HMM-score: 16.7)GATase_3; CobB/CobQ-like glutamine amidotransferase domain (PF07685; HMM-score: 16)no clan defined AIDA; Adhesin of bacterial autotransporter system, probable stalk (PF16168; HMM-score: 15.2)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.010579
- TAT(Tat/SPI): 0.000386
- LIPO(Sec/SPII): 0.000992
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MTKKVAIILANEFEDIEYSSPKEALENAGFNTVVIGDTANSEVVGKHGEKVTVDVGIAEAKPEDYDALLIPGGFSPDHLRGDTEGRYGTFAKYFTKNDVPTFAICHGPQILIDTDDLKGRTLTAVLNVRKDLSNAGAHVVDESVVVDNNIVTSRVPDDLDDFNREIVKQLQ
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: SigB see NWMN_1767
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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