PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS09850 [old locus tag: NWMN_1748 ]
• pan locus tag^?: SAUPAN004792000
• symbol: NWMN_RS09850
• pan gene symbol^?: queG
• synonym:
• product: epoxyqueuosine reductase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS09850 [old locus tag: NWMN_1748 ]
• symbol: NWMN_RS09850
• product: epoxyqueuosine reductase
• replicon: chromosome
• strand: -
• coordinates: 1948916..1950058
• length: 1143
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (1948916..1950058) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_1748

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  
  ATGGGGGGCATACGATTGGATACAAAGCAATTAAAGCAAGATATCATTGACTATGCATAT
  ACAATTGGTATCGACAGTATTGGATTTACTACTGCCGATCCCTTTGATGAATTGAAGCAA
  AAGCTAGAAGCATATCATGCAAATGGTTATGCCTCAGGATTTGAAGAATCTGATATTGCT
  TTACGAACGGAGCCTAAATTATCCTTACCAACAGCAAGGTCAATCATAGCAATTGCAGTT
  GGTTATCCTAACAAACTGAAAGGTGCACCTAAGAGTGTTAGAGGTGATCGCAGAGGCTTA
  TTTGCTAGAGCATCGTGGGGTCAAGATTATCATACAATTATGCGTAAACGATTAGACATG
  TTAGCTGCATTTATTGAATCTAAAGTTCCAGATGTTGAAATCAAATCTATGGTAGATACG
  GGTGTATTATCAGATAGGGCAGTAGCAGAACGTGCAGGTTTAGGATTTGTTGGTCGTAAT
  GGCTTTGTCATCAATCCTAAACTAGGAACATGGACATACCTCGGTGAAATGTTAGTCAGT
  ATACCTTTTGAACCTGATGATCCATTATTAGATAGCTGTGGTGATTGTACAATTTGTGTT
  GATCGTTGTCCAACAAGTGCATTAGTTGGAAATGGCCAATTAAATAGTCAGAAATGCATT
  AGTTTTTTAACGCAAACAAAAGGCTATATGCCTGACCAATATCGTTATAAAATTGGGAAT
  AGATTATATGGTTGCGATACGTGTCAACAAGTTTGTCCGAAAAATAGAGGTATTAATACC
  GAACAAGATGACATCATTTTGGAACCAGAAATTTTAAAGCCAAGATTAGTACCTTTACTA
  CGTATGTCTAATAAAGAATTCAAACAAACATATGGTCACCTTGCAGGTGCTTGGCGTGGT
  AAAAAGCCGATACAACGAAATGCTATTTTAGCATTGGCGCATTTTAATGAAGTGGATGCA
  ATTCCAGAATTGAAAAAAGTTGCAACAACAGATGAAAGACCGTTGATTCGAGCAACTGCT
  TATTGGGCCATTGGTCAAATTCTTGGGGAAGAAGCAAGAGATTTTATCAATGCTAATTAT
  GATCAAGAGGATGCAGAAGTTCAGAATGAAATGATAAAAGGATTAGACACAAGGAGAGAA
  TAG
  60
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  960
  1020
  1080
  1140
  1143
  

⊟Protein

⊟General

• locus tag: NWMN_RS09850 [old locus tag: NWMN_1748 ]
• symbol: NWMN_RS09850
• description: epoxyqueuosine reductase
• length: 380
• theoretical pI: 6.52454
• theoretical MW: 42432.2
• GRAVY: -0.362632

⊟Function

• reaction:
  EC 1.17.99.6?  ExPASy
  Epoxyqueuosine reductase Queuosine³⁴ in tRNA + acceptor + H₂O = epoxyqueuosine³⁴ in tRNA + reduced acceptor
• TIGRFAM:
  Genetic information processing Protein synthesis tRNA and rRNA base modification epoxyqueuosine reductase (TIGR00276; EC 1.-.-.-; HMM-score: 401.2)
  and 10 more
  reductive dehalogenase (TIGR02486; EC 1.97.1.-; HMM-score: 45.1)
  2-oxoacid:acceptor oxidoreductase, delta subunit, pyruvate/2-ketoisovalerate family (TIGR02179; HMM-score: 19.8)
  Metabolism Energy metabolism Electron transport electron transport complex, RnfABCDGE type, B subunit (TIGR01944; HMM-score: 15.5)
  Metabolism Energy metabolism Electron transport NADH-quinone oxidoreductase, chain I (TIGR01971; HMM-score: 11.6)
  Metabolism Energy metabolism Chemoautotrophy CO dehydrogenase/acetyl-CoA synthase complex, epsilon subunit (TIGR00314; EC 1.2.99.2; HMM-score: 10.7)
  Unknown function General archaeoflavoprotein, MJ0208 family (TIGR02700; HMM-score: 10.4)
  pyruvate:ferredoxin (flavodoxin) oxidoreductase (TIGR02176; EC 1.2.7.1; HMM-score: 7.7)
  Genetic information processing Protein fate Protein modification and repair glycine radical enzyme activase, YjjW family (TIGR04041; EC 1.97.1.-; HMM-score: 6.9)
  Metabolism Central intermediary metabolism Nitrogen fixation ferredoxin III, nif-specific (TIGR02936; HMM-score: 5.3)
  methylamine methyltransferase corrinoid protein reductive activase (TIGR04270; HMM-score: 3.7)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  no clan defined DUF1730; Domain of unknown function (DUF1730) (PF08331; HMM-score: 62.9)
  4Fe-4S (CL0344) Fer4_16; 4Fe-4S double cluster binding domain (PF13484; HMM-score: 54.7)
  and 16 more
  TPR (CL0020) HEAT_2; HEAT repeats (PF13646; HMM-score: 32)
  4Fe-4S (CL0344) Fer4_9; 4Fe-4S dicluster domain (PF13187; HMM-score: 26.1)
  Fer4_7; 4Fe-4S dicluster domain (PF12838; HMM-score: 24.7)
  Fer4_10; 4Fe-4S dicluster domain (PF13237; HMM-score: 24.1)
  Fer4; 4Fe-4S binding domain (PF00037; HMM-score: 18.5)
  Fer4_21; 4Fe-4S dicluster domain (PF14697; HMM-score: 17.8)
  Fer4_6; 4Fe-4S binding domain (PF12837; HMM-score: 14.8)
  TPR (CL0020) HEAT_PBS; PBS lyase HEAT-like repeat (PF03130; HMM-score: 13.1)
  HEAT; HEAT repeat (PF02985; HMM-score: 12.8)
  FMN-dep-NRtase (CL0529) Dehalogenase; Reductive dehalogenase subunit (PF13486; HMM-score: 12.6)
  4Fe-4S (CL0344) Fer4_2; 4Fe-4S binding domain (PF12797; HMM-score: 12.4)
  Fer4_8; 4Fe-4S dicluster domain (PF13183; HMM-score: 12.3)
  Fer4_3; 4Fe-4S binding domain (PF12798; HMM-score: 11.6)
  Fer4_17; 4Fe-4S dicluster domain (PF13534; HMM-score: 11.3)
  Fer4_13; 4Fe-4S single cluster domain of Ferredoxin I (PF13370; HMM-score: 10)
  Fer4_18; 4Fe-4S dicluster domain (PF13746; HMM-score: 7.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 1.78
  • Cytoplasmic Membrane Score: 8.16
  • Cellwall Score: 0.06
  • Extracellular Score: 0.01
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.006812
  • TAT(Tat/SPI): 0.000512
  • LIPO(Sec/SPII): 0.00084
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: see NWMN_1748
• RefSeq: WP_001789049 NCBI
• UniProt: see NWMN_1748

⊟Protein sequence

• MGGIRLDTKQLKQDIIDYAYTIGIDSIGFTTADPFDELKQKLEAYHANGYASGFEESDIALRTEPKLSLPTARSIIAIAVGYPNKLKGAPKSVRGDRRGLFARASWGQDYHTIMRKRLDMLAAFIESKVPDVEIKSMVDTGVLSDRAVAERAGLGFVGRNGFVINPKLGTWTYLGEMLVSIPFEPDDPLLDSCGDCTICVDRCPTSALVGNGQLNSQKCISFLTQTKGYMPDQYRYKIGNRLYGCDTCQQVCPKNRGINTEQDDIILEPEILKPRLVPLLRMSNKEFKQTYGHLAGAWRGKKPIQRNAILALAHFNEVDAIPELKKVATTDERPLIRATAYWAIGQILGEEARDFINANYDQEDAEVQNEMIKGLDTRRE

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications