From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS09230 [old locus tag: NWMN_1644 ]
  • pan locus tag?: SAUPAN004429000
  • symbol: NWMN_RS09230
  • pan gene symbol?: —
  • synonym:
  • product: dipeptidase PepV

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS09230 [old locus tag: NWMN_1644 ]
  • symbol: NWMN_RS09230
  • product: dipeptidase PepV
  • replicon: chromosome
  • strand: -
  • coordinates: 1831525..1832934
  • length: 1410
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (1831525..1832934) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_1644

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTGGAAAGAAAAAGTTCAACAATACGAAGATCAAATCATTAATGACTTAAAAGGATTA
    TTAGCAATTGAAAGTGTGAGAGATGATGCAAAAGCATCAGAAGACGCACCAGTTGGTCCA
    GGTCCTCGTAAGGCATTAGACTACATGTATGAAATTGCACATAGAGATGGATTTACAACA
    CATGATGTGGATCATATTGCAGGAAGAATTGAGGCAGGTAAAGGAAATGACGTATTAGGT
    ATCTTATGTCATGTTGACGTTGTTCCTGCTGGTGATGGATGGGATAGTAATCCGTTCGAG
    CCGGTTGTAACAGAAGATGCTATCATAGCTAGAGGTACACTTGATGACAAAGGTCCAACA
    ATTGCTGCTTATTATGCAATTAAGATATTAGAAGATATGAATGTGGATTGGAAGAAACGT
    ATTCATATGATTATTGGTACGGATGAAGAATCTGATTGGAAATGTACGGATCGCTATTTT
    AAAACAGAAGAAATGCCAACATTAGGTTTTGCACCAGATGCAGAATTTCCATGTATTCAT
    GGTGAAAAAGGCATTACAACATTTGATTTAGTTCAAAATAAACTTACTGAAGATCAAGAT
    GAACCTGATTATGAATTAATAACTTTTAAATCTGGTGAACGTTACAACATGGTACCTGAT
    CATGCAGAAGCAAGAGTGCTTGTTAAAGAAAATATGACAGATGTTATTCAAGACTTTGAG
    TACTTTTTAGAACAAAATCATTTACAAGGTGATAGTACTGTTGATAGTGGCATTCTAGTT
    TTAACAGTTGAAGGTAAAGCGGTTCATGGTATGGATCCATCTATCGGTGTGAATGCGGGT
    CTTTACTTACTAAAATTCTTAGCATCATTAAATCTTGATAATAATGCACAAGCGTTTGTA
    GCATTTAGTAATCGCTACTTATTTAATTCAGATTTTGGTGAAAAGATGGGAATGAAATTC
    CATACAGATGTCATGGGTGACGTGACAACTAACATTGGTGTTATTACATATGATAATGAA
    AACGCAGGTCTTTTCGGTATCAACTTACGCTACCCAGAAGGATTTGAATTTGAAAAAGCT
    ATGGATCGTTTTGCAAATGAGATTCAACAATATGGCTTTGAAGTGAAATTAGGTAAAGTC
    CAACCACCACATTATGTTGATAAAAATGATCCTTTTGTACAAAAGTTAGTTACTGCATAT
    AGAAATCAAACAAATGATATGACTGAACCTTATACTATAGGTGGCGGTACTTATGCGAGA
    AACTTAGACAAGGGTGTAGCATTTGGCGCAATGTTTAGTGATTCTGAAGATTTAATGCAT
    CAGAAAAATGAATATATCACTAAAAAACAGTTATTTAACGCAACTAGTATTTACTTAGAA
    GCAATTTATTCATTATGCGTGGAGGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1410

⊟Protein[edit | edit source]

Protein Data Bank: 3KHX
Protein Data Bank: 3KHZ
Protein Data Bank: 3KI9

⊟General[edit | edit source]

  • locus tag: NWMN_RS09230 [old locus tag: NWMN_1644 ]
  • symbol: NWMN_RS09230
  • description: dipeptidase PepV
  • length: 469
  • theoretical pI: 4.32275
  • theoretical MW: 52823.9
  • GRAVY: -0.408316

⊟Function[edit | edit source]

  • TIGRFAM:
    putative dipeptidase (TIGR01887; EC 3.4.13.-; HMM-score: 637.7)
    and 11 more
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides dipeptidase PepV (TIGR01886; EC 3.4.13.-; HMM-score: 459.3)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase, ArgE/DapE family (TIGR01910; EC 3.4.-.-; HMM-score: 105.3)
    Metabolism Amino acid biosynthesis Aspartate family succinyl-diaminopimelate desuccinylase (TIGR01246; EC 3.5.1.18; HMM-score: 62.3)
    putative selenium metabolism hydrolase (TIGR03526; HMM-score: 45)
    N-acyl-L-amino-acid amidohydrolase (TIGR01880; EC 3.5.1.14; HMM-score: 43.4)
    Metabolism Amino acid biosynthesis Glutamate family acetylornithine deacetylase (ArgE) (TIGR01892; EC 3.5.1.16; HMM-score: 40.5)
    peptidase T-like protein (TIGR01883; HMM-score: 30.6)
    N-acetyl-ornithine/N-acetyl-lysine deacetylase (TIGR01902; HMM-score: 30.6)
    Metabolism Amino acid biosynthesis Aspartate family succinyl-diaminopimelate desuccinylase (TIGR01900; EC 3.5.1.18; HMM-score: 19.6)
    Xaa-His dipeptidase (TIGR01893; EC 3.4.13.20; HMM-score: 14.9)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides amidohydrolase (TIGR01891; HMM-score: 11.8)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    Peptidase_MH (CL0035) Peptidase_M20; Peptidase family M20/M25/M40 (PF01546; HMM-score: 97.4)
    and 2 more
    no clan defined M20_dimer; Peptidase dimerisation domain (PF07687; HMM-score: 32.4)
    Peptidase_MH (CL0035) Peptidase_M28; Peptidase family M28 (PF04389; HMM-score: 13)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.014046
    • TAT(Tat/SPI): 0.001237
    • LIPO(Sec/SPII): 0.001764
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MWKEKVQQYEDQIINDLKGLLAIESVRDDAKASEDAPVGPGPRKALDYMYEIAHRDGFTTHDVDHIAGRIEAGKGNDVLGILCHVDVVPAGDGWDSNPFEPVVTEDAIIARGTLDDKGPTIAAYYAIKILEDMNVDWKKRIHMIIGTDEESDWKCTDRYFKTEEMPTLGFAPDAEFPCIHGEKGITTFDLVQNKLTEDQDEPDYELITFKSGERYNMVPDHAEARVLVKENMTDVIQDFEYFLEQNHLQGDSTVDSGILVLTVEGKAVHGMDPSIGVNAGLYLLKFLASLNLDNNAQAFVAFSNRYLFNSDFGEKMGMKFHTDVMGDVTTNIGVITYDNENAGLFGINLRYPEGFEFEKAMDRFANEIQQYGFEVKLGKVQPPHYVDKNDPFVQKLVTAYRNQTNDMTEPYTIGGGTYARNLDKGVAFGAMFSDSEDLMHQKNEYITKKQLFNATSIYLEAIYSLCVEE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    NWMN_RS02105alkyl hydroperoxide reductase subunit C  [1] (data from MRSA252)
    NWMN_RS02155GMP synthase (glutamine-hydrolyzing)  [1] (data from MRSA252)
    NWMN_RS04915NAD(+) kinase  [1] (data from MRSA252)
    NWMN_RS05000bifunctional metallophosphatase/5'-nucleotidase  [1] (data from MRSA252)
    NWMN_RS09225D-alanine aminotransferase  [1] (data from MRSA252)
    NWMN_RS1239530S ribosomal protein S3  [1] (data from MRSA252)
    NWMN_RS14560arginine deiminase  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=NWMN_RS09230&oldid=61099"