From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS08355 [old locus tag: NWMN_1484 ]
  • pan locus tag?: SAUPAN004164000
  • symbol: NWMN_RS08355
  • pan gene symbol?: grpE
  • synonym:
  • product: nucleotide exchange factor GrpE

⊟Additional information (user-provided)[edit | edit source]

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS08355 [old locus tag: NWMN_1484 ]
  • symbol: NWMN_RS08355
  • product: nucleotide exchange factor GrpE
  • replicon: chromosome
  • strand: -
  • coordinates: 1651313..1651939
  • length: 627
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (1651313..1651939) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_1484

⊟Phenotype[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGACAAATAAAGACGAATCAGTTGAAAAAAACACTGAATCAACAGTTGAAGAAACAAAC
    GTCAAACAAAATATTGATGATTCAGTTGAACAAGCTGAAGAAAGTAAAGGTCATTTACAA
    GATGAAGCAATAGAAGAAACGTCTGACGAGAATGTTATTGAAGAAATAGATCCAAAAGAT
    CAAAAAATTAATGAACTTCAACAATTAGCAGATGAAAACGAAGAGAAATATTTAAGGCTC
    TACGCTGAGTTTGAAAATTATAAGCGTAGAATTCAAAAAGAAAATGAAATAAACAAAACA
    TATCAAGCACAACGTGTGTTAACAGATATTTTACCAGCAATAGACAATATAGAACGTGCA
    CTTCAAATTGAAGGTGATGATGAGACTTTTAAATCTCTTCAAAAAGGTGTACAAATGGTG
    CATGAAAGTTTGATTAACGCACTAAAAGATAATGGTCTTGAAGTTATTAAAACTGAAGGT
    GAAGCATTTGATCCAAATATTCACCAAGCTGTAGTTCAAGATGATAACCCTGATTTTGAA
    TCTGGCGAAATCACTCAAGAACTACAAAAAGGATACAAGCTTAAAGATAGAGTATTAAGA
    CCATCAATGGTCAAAGTAAACCAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    627


⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS08355 [old locus tag: NWMN_1484 ]
  • symbol: NWMN_RS08355
  • description: nucleotide exchange factor GrpE
  • length: 208
  • theoretical pI: 4.14169
  • theoretical MW: 24008.2
  • GRAVY: -1.03413

⊟Function[edit | edit source]

  • TIGRFAM:
    chain length determinant protein EpsF (TIGR03017; HMM-score: 9.6)
  • TheSEED: see NWMN_1484
  • PFAM:
    no clan defined GrpE; GrpE (PF01025; HMM-score: 164.7)
    and 2 more
    DUF6827; Domain of unknown function (DUF6827) (PF20715; HMM-score: 10.3)
    DUF5856; Family of unknown function (DUF5856) (PF19174; HMM-score: 8.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.996
    • Cytoplasmic Membrane Score: 0.0007
    • Cell wall & surface Score: 0.0003
    • Extracellular Score: 0.003
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00891
    • TAT(Tat/SPI): 0.00396
    • LIPO(Sec/SPII): 0.002874
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTNKDESVEKNTESTVEETNVKQNIDDSVEQAEESKGHLQDEAIEETSDENVIEEIDPKDQKINELQQLADENEEKYLRLYAEFENYKRRIQKENEINKTYQAQRVLTDILPAIDNIERALQIEGDDETFKSLQKGVQMVHESLINALKDNGLEVIKTEGEAFDPNIHQAVVQDDNPDFESGEITQELQKGYKLKDRVLRPSMVKVNQ

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟Other information (user-provided)[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]


⊟Relevant publications[edit | edit source]