PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS08125 [old locus tag: NWMN_1440 ]
• pan locus tag^?: SAUPAN004113000
• symbol: NWMN_RS08125
• pan gene symbol^?: gcvPA
• synonym:
• product: glycine dehydrogenase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS08125 [old locus tag: NWMN_1440 ]
• symbol: NWMN_RS08125
• product: glycine dehydrogenase
• replicon: chromosome
• strand: -
• coordinates: 1610250..1611596
• length: 1347
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (1610250..1611596) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_1440

⊟Phenotype

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⊟DNA sequence

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  GTGAGTCATCGTTATATACCTTTAACTGAAAAAGACAAGCAAGAAATGTTACAAACAATT
  GGTGCAAAATCTATAGGAGAATTATTCGGTGATGTACCAAGTGACATTTTATTAAATAGA
  GATTTAAATATTGCTGAAGGCGAAGCGGAAACAACGTTACTTAGAAGATTAAATCGCATT
  GCAAGCAAGAATATCACTAAAGAAACGCATACATCATTTTTGGGTGCTGGTGTTTATGAT
  CATTACGCGCCATCAGTTGTGGATGCTATGATTTCAAGATCAGAATTTTACACAGCATAT
  ACGCCATATCAACCGGAAATTTCTCAAGGTGAATTACAAGCCATTTTTGAGTTCCAAACT
  TTAATTTGTGAATTAACAGATATGGATGTAGCAAATTCTTCTATGTATGATGGGATGACT
  AGCTTTGCTGAAGCATGTATCTTAGCATTTAGTCAAACAAAGAAAAATAAAATTGTAGTC
  TCAAAAGGTTTACATTATCAAGCATTACAAGTACTTCATACTTATGCTAAGACGCGTAAG
  GAATTCGAAGTAGTGGAAATCGATTTAGATGGCACTGTTACAGATTTGAAAAAATTAGAA
  GCAGCAGTGGATGATGAAACAGCAGCTGTTGCAGTACAATATCCAAACTTTTACGGTTCA
  ATTGAAGATCTTGAAAAGATTCATAGCTTTATTGAAGATAAAAAAGCATTATTTATCGTA
  TATGCAAATCCATTAGCATTGGGATTACTTACACCTCCAGGTTCATTTGGTGCAGATATT
  GTTGTAGGTGATACTCAGCCATTCGGAATTCCTGCTCAATTCGGTGGGCCACATTGTGGA
  TATTTTGCAACGACGAAAAAGTTAATGCGTAAAGTGCCAGGTAGATTAGTCGGTCAAACA
  CAAGATGATGAAGGTAATCGTGGCTTTGTATTAACATTACAAGCACGTGAACAACATATT
  CGACGCGATAAAGCGACATCTAATATTTGTTCTAACCAAGCATTAAATGCTTTAGCGTCA
  TCAATTGCAATGTCAGCACTTGGTAAACAAGGTATCTATGATATTGCAGTTCAAAATATA
  GAACATGCTAACTATGCTAAACAACAATTTATTAAAAAAGGTTTTGAAGTATTAGATGGT
  ACATCTTTCAATGAGTTTGTAGTCAAATTTGACAAACCAATTCAACAAGTTAACGAGGAA
  TTAGTGAAGTACAATATTATCGGTGGTTTTGATTTAGGTGTTGTTTCAGACGACTTTAAA
  AATCACATGTTAATAGCTGTAACTGAATTAAGAACAAAAGATGAAATCGATACATTTGTA
  GAAAAGGCTGGTGAGTTAAATGACTAG
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⊟Protein

⊟General

• locus tag: NWMN_RS08125 [old locus tag: NWMN_1440 ]
• symbol: NWMN_RS08125
• description: glycine dehydrogenase
• length: 448
• theoretical pI: 4.83131
• theoretical MW: 49716.1
• GRAVY: -0.201339

⊟Function

• reaction:
  EC 1.4.4.2?  ExPASy
  Glycine dehydrogenase (aminomethyl-transferring) Glycine + [glycine-cleavage complex H protein]-N⁶-lipoyl-L-lysine = [glycine-cleavage complex H protein]-S-aminomethyl-N⁶-dihydrolipoyl-L-lysine + CO₂
• TIGRFAM:
  Metabolism Energy metabolism Amino acids and amines glycine dehydrogenase (TIGR00461; EC 1.4.4.2; HMM-score: 316.7)
  and 8 more
  cysteine desulfurase NifS (TIGR03402; EC 2.8.1.7; HMM-score: 27.3)
  Genetic information processing Protein synthesis tRNA and rRNA base modification cysteine desulfurase IscS (TIGR02006; EC 2.8.1.7; HMM-score: 25.2)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteine desulfurase IscS (TIGR02006; EC 2.8.1.7; HMM-score: 25.2)
  Unknown function Enzymes of unknown specificity cysteine desulfurase family protein (TIGR01977; HMM-score: 25)
  Genetic information processing DNA metabolism Restriction/modification cysteine desulfurase DndA (TIGR03235; EC 2.8.1.7; HMM-score: 19.9)
  Metabolism Energy metabolism Amino acids and amines tyrosine aminotransferase (TIGR01264; EC 2.6.1.5; HMM-score: 14.6)
  tyrosine/nicotianamine family aminotransferase (TIGR01265; HMM-score: 12)
  cysteine desulfurase, NifS family (TIGR03403; EC 2.8.1.7; HMM-score: 11.9)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  PLP_aminotran (CL0061) GDC-P; Glycine cleavage system P-protein (PF02347; HMM-score: 506.5)
  and 1 more
  Aminotran_5; Aminotransferase class-V (PF00266; HMM-score: 22.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003023
  • TAT(Tat/SPI): 0.000738
  • LIPO(Sec/SPII): 0.000537
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 445941832 NCBI
• RefSeq: WP_000019687 NCBI
• UniProt: see NWMN_1440

⊟Protein sequence

• MSHRYIPLTEKDKQEMLQTIGAKSIGELFGDVPSDILLNRDLNIAEGEAETTLLRRLNRIASKNITKETHTSFLGAGVYDHYAPSVVDAMISRSEFYTAYTPYQPEISQGELQAIFEFQTLICELTDMDVANSSMYDGMTSFAEACILAFSQTKKNKIVVSKGLHYQALQVLHTYAKTRKEFEVVEIDLDGTVTDLKKLEAAVDDETAAVAVQYPNFYGSIEDLEKIHSFIEDKKALFIVYANPLALGLLTPPGSFGADIVVGDTQPFGIPAQFGGPHCGYFATTKKLMRKVPGRLVGQTQDDEGNRGFVLTLQAREQHIRRDKATSNICSNQALNALASSIAMSALGKQGIYDIAVQNIEHANYAKQQFIKKGFEVLDGTSFNEFVVKFDKPIQQVNEELVKYNIIGGFDLGVVSDDFKNHMLIAVTELRTKDEIDTFVEKAGELND

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: Gly-box see NWMN_1440

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications