PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS08085 [old locus tag: NWMN_1432 ]
• pan locus tag^?: SAUPAN004082000
• symbol: NWMN_RS08085
• pan gene symbol^?: accB
• synonym:
• product: acetyl-CoA carboxylase biotin carboxyl carrier protein subunit

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS08085 [old locus tag: NWMN_1432 ]
• symbol: NWMN_RS08085
• product: acetyl-CoA carboxylase biotin carboxyl carrier protein subunit
• replicon: chromosome
• strand: -
• coordinates: 1603482..1603946
• length: 465
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (1603482..1603946) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_1432

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAACTTTAAAGAAATCAAAGAATTAATTGAAATTCTGGATAAATCAACTTTAACGGAA
  ATCAATATTGAAGATACTAAAGGCAAAGTGACGCTTAAGAAAGAAAAAGAAACTGAGATT
  ATCACGCCACAAATCTCACAAATGCCAGTTGAAGCTGCGGCAATGCCTATGCCTCAAGCA
  CAATCAACTGATAGCAATAAAACTGAAGCTCCAAAGCCAACTTCAGATAATCACAAAACA
  ATTAATGCACCTATGGTAGGTACATTTTACAAATCGCCATCTCCAGACGAAGAAGCATAT
  GTGCAAGTTGGGGACACTGTTTCAAATGAAACAACAGTGTGTATTTTAGAGGCAATGAAA
  CTATTTAATGAAATTCAAGCAGAAATTTCAGGTGAAATTGTTGAAATCTTAGTAGAAGAC
  GGACAAATGGTAGAGTATGGCCAACCGTTATTTAAGGTGAAATAA
  60
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  420
  465
  

⊟Protein

⊟General

• locus tag: NWMN_RS08085 [old locus tag: NWMN_1432 ]
• symbol: NWMN_RS08085
• description: acetyl-CoA carboxylase biotin carboxyl carrier protein subunit
• length: 154
• theoretical pI: 4.2253
• theoretical MW: 17121.4
• GRAVY: -0.423377

⊟Function

• TIGRFAM:
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis acetyl-CoA carboxylase, biotin carboxyl carrier protein (TIGR00531; HMM-score: 170.6)
  and 16 more
  Metabolism Central intermediary metabolism Nitrogen metabolism urea carboxylase (TIGR02712; EC 6.3.4.6; HMM-score: 39.4)
  Metabolism Transport and binding proteins Cations and iron carrying compounds oxaloacetate decarboxylase alpha subunit (TIGR01108; EC 4.1.1.3; HMM-score: 38.5)
  Metabolism Energy metabolism Other oxaloacetate decarboxylase alpha subunit (TIGR01108; EC 4.1.1.3; HMM-score: 38.5)
  Metabolism Energy metabolism Pyruvate dehydrogenase dihydrolipoyllysine-residue acetyltransferase (TIGR01348; EC 2.3.1.12; HMM-score: 31.1)
  Metabolism Energy metabolism Glycolysis/gluconeogenesis pyruvate carboxylase (TIGR01235; EC 6.4.1.1; HMM-score: 30.1)
  Metabolism Energy metabolism TCA cycle dihydrolipoyllysine-residue succinyltransferase, E2 component of oxoglutarate dehydrogenase (succinyl-transferring) complex (TIGR01347; EC 2.3.1.61; HMM-score: 22.2)
  Metabolism Transport and binding proteins Unknown substrate efflux transporter, RND family, MFP subunit (TIGR01730; HMM-score: 22)
  Cellular processes Cellular processes Biosynthesis of natural products NHLM bacteriocin system secretion protein (TIGR03794; HMM-score: 16.3)
  Metabolism Transport and binding proteins Amino acids, peptides and amines NHLM bacteriocin system secretion protein (TIGR03794; HMM-score: 16.3)
  Metabolism Transport and binding proteins Other efflux pump membrane protein (TIGR00998; HMM-score: 15)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking type I secretion membrane fusion protein, HlyD family (TIGR01843; HMM-score: 14.9)
  Cell structure Cell envelope Other uncharacterized lipoprotein (TIGR02722; HMM-score: 14.7)
  glycine cleavage protein H-like protein (TIGR03077; HMM-score: 13.7)
  Metabolism Energy metabolism Amino acids and amines glycine cleavage system H protein (TIGR00527; HMM-score: 13.3)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair UV excision repair protein Rad23 (TIGR00601; HMM-score: 13)
  2-oxoglutarate dehydrogenase, E2 component, dihydrolipoamide succinyltransferase (TIGR02927; EC 2.3.1.61; HMM-score: 10.2)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  Hybrid (CL0105) Biotin_lipoyl; Biotin-requiring enzyme (PF00364; HMM-score: 80.6)
  and 6 more
  Biotin_lipoyl_2; Biotin-lipoyl like (PF13533; HMM-score: 30.1)
  GCV_H; Glycine cleavage H-protein (PF01597; HMM-score: 20.7)
  HlyD_3; HlyD family secretion protein (PF13437; HMM-score: 19.1)
  HlyD_D23; Barrel-sandwich domain of CusB or HlyD membrane-fusion (PF16576; HMM-score: 14.8)
  RnfC_N; RnfC Barrel sandwich hybrid domain (PF13375; HMM-score: 12.2)
  no clan defined Dicty_REP; Dictyostelium (Slime Mold) REP protein (PF05086; HMM-score: 11.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.063844
  • TAT(Tat/SPI): 0.002354
  • LIPO(Sec/SPII): 0.001119
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446932260 NCBI
• RefSeq: WP_001009516 NCBI
• UniProt: see NWMN_1432

⊟Protein sequence

• MNFKEIKELIEILDKSTLTEINIEDTKGKVTLKKEKETEIITPQISQMPVEAAAMPMPQAQSTDSNKTEAPKPTSDNHKTINAPMVGTFYKSPSPDEEAYVQVGDTVSNETTVCILEAMKLFNEIQAEISGEIVEILVEDGQMVEYGQPLFKVK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications