PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS07345 [old locus tag: NWMN_1304 ]
• pan locus tag^?: SAUPAN003806000
• symbol: NWMN_RS07345
• pan gene symbol^?: lysC
• synonym:
• product: aspartate kinase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS07345 [old locus tag: NWMN_1304 ]
• symbol: NWMN_RS07345
• product: aspartate kinase
• replicon: chromosome
• strand: +
• coordinates: 1436783..1437988
• length: 1206
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (1436783..1437988) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_1304

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  
  ATGGTAACAAGAAGTGTGTTGAAATTTGGCGGATCATCCGTCAGTGATTTTACAAAAATA
  AAAAGGATCGCTGAAATGTTAAAGGAGCGAGTCAATCAAGATGAACAATTAATTGTCGTT
  GTAAGTGCTATGGGTAACACAACAGATCAATTAATGACGAATGTATCAACCTTGACTAAA
  GCACCAAAACAACAAGAACTGGCATTATTATTGACAACCGGAGAGCAACAAACTGTATCT
  TATTTATCAATGGTATTAAATGATATCGGTATGAATGCCAAAGCAATGACTGGCTATCAA
  GCGGGTATTAAAACCATTGGCCATCATTTAAAAAGTAAAATTGCTCAAATTAATCCTCAA
  ACATTTGAACAAGCCTTTCAAGAAAACGATATTTTAGTAGTTGCTGGATTTCAAGGCATC
  AATGAACATCAGGAATTAACAACTTTAGGCAGAGGTGGTTCTGATACGACCGCTGTGGCA
  CTTGCTGTTAGTAATCAAATACCTTGTGAAATTTATACCGACGTTGATGGTGTGTATGCC
  ACTGACCCAAGACTTTTACCAAAGGCTAAACGACTAGACATCGTCTCATATGAAGAAATG
  ATGGAAATGAGCGCTTTAGGTGCTGGTGTACTTGAAACAAGAAGTGTTGAATTAGCTAAA
  AACTATAATATCCCTTTATATTTAGGAAAAACTTTATCGAACGTGAAAGGAACATGGATT
  ATGTCAAATGAAGAAATATTAGAGAAAAAAGCAGTTACTGGTGTGGCTTTGGATAAACAT
  ATGATGCATGTAACAATTAGTTATCCCCTACCTGACAATCAGCTACTTACCCAACTATTT
  ACGGAACTTGAAGAAGGTGCTGTAAATGTTGATATGATTTCACAAATCGTCAACTTGGAT
  GGGCTACAACTATCCTTCACGATTAAAGATAGTGATTTTCATCAAATTTCTATGATTCTT
  GAAACATTAAAGAATCAATATGAAGCATTAGCTTATAAAATCAATGAGCATTATGTCAAA
  ATTTCATTAATTGGCTCAGGCATGCGTGATATGTCAGGTGTGGCATCAAAAGCATTTTTG
  ACATTAATTGAAAATAATATACCTTTCTACCAAACAACAACATCTGAAATAAGTATTTCA
  TACGTCATTGATGATTTTAATGGGCAACAAGCGGTAGAAAAACTATATGACGCATTTAAC
  ATTTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1206
  

⊟Protein

⊟General

• locus tag: NWMN_RS07345 [old locus tag: NWMN_1304 ]
• symbol: NWMN_RS07345
• description: aspartate kinase
• length: 401
• theoretical pI: 4.70763
• theoretical MW: 44374.6
• GRAVY: -0.0591022

⊟Function

• reaction:
  EC 2.7.2.4?  ExPASy
  Aspartate kinase ATP + L-aspartate = ADP + 4-phospho-L-aspartate
• TIGRFAM:
  Metabolism Amino acid biosynthesis Aspartate family aspartate kinase (TIGR00657; EC 2.7.2.4; HMM-score: 354)
  Metabolism Amino acid biosynthesis Aspartate family aspartate kinase, monofunctional class (TIGR00656; EC 2.7.2.4; HMM-score: 330.8)
  and 4 more
  Metabolism Amino acid biosynthesis Aspartate family putative Pyrococcus aspartate kinase subunit (TIGR02078; EC 2.7.2.4; HMM-score: 105.7)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions UMP kinase (TIGR02075; EC 2.7.4.22; HMM-score: 36.4)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions putative uridylate kinase (TIGR02076; EC 2.7.4.-; HMM-score: 31.7)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiamine pyrophosphokinase (TIGR01378; EC 2.7.6.2; HMM-score: 16.1)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  no clan defined AA_kinase; Amino acid kinase family (PF00696; HMM-score: 148.1)
  and 3 more
  ACT (CL0070) ACT_7; ACT domain (PF13840; HMM-score: 27)
  no clan defined TPK_catalytic; Thiamin pyrophosphokinase, catalytic domain (PF04263; HMM-score: 20.3)
  E-set (CL0159) Alpha_adaptinC2; Adaptin C-terminal domain (PF02883; HMM-score: 14.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004219
  • TAT(Tat/SPI): 0.000769
  • LIPO(Sec/SPII): 0.000608
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446180857 NCBI
• RefSeq: WP_000258712 NCBI
• UniProt: see NWMN_1304

⊟Protein sequence

• MVTRSVLKFGGSSVSDFTKIKRIAEMLKERVNQDEQLIVVVSAMGNTTDQLMTNVSTLTKAPKQQELALLLTTGEQQTVSYLSMVLNDIGMNAKAMTGYQAGIKTIGHHLKSKIAQINPQTFEQAFQENDILVVAGFQGINEHQELTTLGRGGSDTTAVALAVSNQIPCEIYTDVDGVYATDPRLLPKAKRLDIVSYEEMMEMSALGAGVLETRSVELAKNYNIPLYLGKTLSNVKGTWIMSNEEILEKKAVTGVALDKHMMHVTISYPLPDNQLLTQLFTELEEGAVNVDMISQIVNLDGLQLSFTIKDSDFHQISMILETLKNQYEALAYKINEHYVKISLIGSGMRDMSGVASKAFLTLIENNIPFYQTTTSEISISYVIDDFNGQQAVEKLYDAFNI

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: L-box, CodY see NWMN_1304

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications