PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS04835 [old locus tag: NWMN_0860 ]
• pan locus tag^?: SAUPAN003137000
• symbol: NWMN_RS04835
• pan gene symbol^?: opp3A
• synonym:
• product: peptide ABC transporter substrate-binding protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS04835 [old locus tag: NWMN_0860 ]
• symbol: NWMN_RS04835
• product: peptide ABC transporter substrate-binding protein
• replicon: chromosome
• strand: +
• coordinates: 954840..956495
• length: 1656
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (954840..956495) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_0860

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGACGAGAAAATTTAGAACACTTATTTTAATTTTGATTGCTACAATTGCATTAAGTGGT
  TGTGCTAATGACGATGGTATTTATTCAGATAAAGGTCAAGTATTCAGAAAAATTTTGTCA
  TCAGACTTAACATCCCTTGATACATCATTAATAACGGATGAAATATCTTCTGAAGTGACT
  GCGCAAACATTCGAAGGTTTATACACATTAGGAAAAGGTGACAAACCGGTGTTAGGTGTT
  GCGAAAGCTTTTCCTGAAAAGAGTAAAGATGGTAAAACTTTAAAGGTTAAATTAAGAAGC
  GATGCTAAATGGAGCAATGGTGACAAAGTGACTGCACAAGACTTTGTTTATGCTTGGAGA
  AAAACAGTTGACCCTAAAACAGGTTCTGAATTTGCATACATTATGGGGGACATTAAAAAT
  GCGAGTGATATTAGTACTGGTAAGAAACCTGTAGAGCAATTAGGTATCAAAGCATTAAAT
  GATGAAACATTACAAATTGAATTAGAAAAGCCGGTTCCATATATTAATCAATTATTAGCA
  CTTAATACCTTTGCACCTCAAAATGAAAAAGTTGCCAAAAAATATGGTAAAAATTACGGT
  ACGGCAGCTGATAGAGCGGTATACAATGGTCCATTTAAAGTTGATGATTGGAAACAAGAA
  GATAAAACCTTACTATCTAAAAATCAGTATTATTGGGATAAAAAGAATGTAAAATTAGAT
  AAAGTGAATTATAAAGTTATTAAAGACTTACAAGCCGGTGCATCATTGTATGATACTGAA
  TCAGTAGATGACGCAGTTATTACTGCAGATCAAGTAAATAAATATAAAGACAACAAAGGA
  TTAAACTTTGTGTTAACGACTGGGACATTTTTTGTAAAAATGAATGAAAAACAATATCCT
  GATTTTAAAAACAAAAATTTAAGATTGGCTATCGCACAAGCAATAGATAAAAAAGGATAC
  GTTGATTCAGTGAAAAACAATGGCTCAATTCCTTCCGATACACTAACAGCCAAAGGAATT
  GCGAAAGCGCCTAATGGCAAAGATTATGCGAGTACCATGAATTCGCCTTTAAAATATAAT
  CCTAAAGAAGCAAGAGCACACTGGGACAAAGCTAAAAAAGAGTTAGGTAAAAATGAAGTG
  ACATTTTCAATGAACACAGAAGATACACCAGATGCAAAAATATCTGCTGAATATATCAAA
  TCGCAAGTTGAGAAAAATTTACCAGGAGTTACTTTGAAAATTAAGCAATTACCGTTTAAA
  CAAAGAGTATCACTAGAACTGAGTAACAATTTTGAAGCATCACTTAGTGGTTGGTCTGCA
  GATTACCCTGATCCTATGGCTTATTTAGAAACAATGACCACAGGTAGCGCACAAAATAAT
  ACAGACTGGGGTAATAAAGAATATGATCAATTACTTAAAGTAGCAAGAACCAAATTGGCA
  CTTCAACCGAACGAACGATATGAAAACTTGAAAAAAGCAGAAGAAATGTTCCTAGGAGAT
  GCACCGGTAGCACCAATTTATCAAAAAGGTGTTGCACATTTAACAAATCCTCAAGTAAAA
  GGATTAATTTACCATAAATTTGGTCCAAATAACTCACTTAAACATGTATATATTGATAAA
  TCGATAGATAAAGAAACAGGTAAGAAGAAAAAATAA
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  1656
  

⊟Protein

⊟General

• locus tag: NWMN_RS04835 [old locus tag: NWMN_0860 ]
• symbol: NWMN_RS04835
• description: peptide ABC transporter substrate-binding protein
• length: 551
• theoretical pI: 9.72302
• theoretical MW: 61571.6
• GRAVY: -0.641924

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, nickel/metallophore periplasmic binding protein (TIGR02294; HMM-score: 68.2)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  PBP (CL0177) SBP_bac_5; Bacterial extracellular solute-binding proteins, family 5 Middle (PF00496; HMM-score: 248.2)
  and 1 more
  LppaM (CL0421) LPAM_1; Prokaryotic membrane lipoprotein lipid attachment site (PF08139; HMM-score: 12.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cellwall
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 9.21
  • Extracellular Score: 0.78
  • Internal Helices: 0
• LocateP:
• SignalP: Signal peptide LIPO(Sec/SPII) length 20 aa
  • SP(Sec/SPI): 0.000807
  • TAT(Tat/SPI): 0.000113
  • LIPO(Sec/SPII): 0.998667
  • Cleavage Site: CS pos: 20-21. LSG-CA. Pr: 0.9996
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446119164 NCBI
• RefSeq: WP_000197019 NCBI
• UniProt: see NWMN_0860

⊟Protein sequence

• MTRKFRTLILILIATIALSGCANDDGIYSDKGQVFRKILSSDLTSLDTSLITDEISSEVTAQTFEGLYTLGKGDKPVLGVAKAFPEKSKDGKTLKVKLRSDAKWSNGDKVTAQDFVYAWRKTVDPKTGSEFAYIMGDIKNASDISTGKKPVEQLGIKALNDETLQIELEKPVPYINQLLALNTFAPQNEKVAKKYGKNYGTAADRAVYNGPFKVDDWKQEDKTLLSKNQYYWDKKNVKLDKVNYKVIKDLQAGASLYDTESVDDAVITADQVNKYKDNKGLNFVLTTGTFFVKMNEKQYPDFKNKNLRLAIAQAIDKKGYVDSVKNNGSIPSDTLTAKGIAKAPNGKDYASTMNSPLKYNPKEARAHWDKAKKELGKNEVTFSMNTEDTPDAKISAEYIKSQVEKNLPGVTLKIKQLPFKQRVSLELSNNFEASLSGWSADYPDPMAYLETMTTGSAQNNTDWGNKEYDQLLKVARTKLALQPNERYENLKKAEEMFLGDAPVAPIYQKGVAHLTNPQVKGLIYHKFGPNNSLKHVYIDKSIDKETGKKKK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CodY see NWMN_0860

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications