PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

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⊟Summary

• organism: Staphylococcus aureus Newman
locus tag: NWMN_RS02465 [old locus tag: NWMN_0437 ]
pan locus tag^?: SAUPAN002187000
symbol: NWMN_RS02465
pan gene symbol^?: gltD
• synonym:
• product: glutamate synthase subunit beta

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS02465 [old locus tag: NWMN_0437 ]
• symbol: NWMN_RS02465
• product: glutamate synthase subunit beta
• replicon: chromosome
• strand: +
• coordinates: 491514..492977
• length: 1464
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (491514..492977) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_0437

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGGGTGAATTTAAAGGATTTATGAAGTATGACAAACAGTACTTAGGTGAATTATCACTG
  GTAGACCGTTTGAAGCATCATAAAGCATATCAACAACGATTTACTAAAGAAGATGCCTCT
  ATCCAAGGTGCACGATGTATGGATTGTGGAACGCCGTTTTGTCAAACCGGACAACAGTAT
  GGTAGGGAAACAATAGGTTGTCCAATTGGAAACTACATTCCTGAATGGAACGACTTAGTG
  TATCATCAAGATTTTAAAACTGCTTATGAACGCTTAAGCGAAACAAATAACTTTCCTGAC
  TTTACAGGGCGTGTATGTCCTGCACCATGCGAAAGTGCTTGTGTGATGAAGATTAATAGA
  GAATCGATTGCGATTAAAGGTATTGAACGCACAATTATTGATGAAGCTTTTGAAAATGGT
  TGGGTAGCGCCGAAAGTTCCGAGTCGCCGTAGAGATGAAAAAGTGGCAATCGTTGGAAGC
  GGTCCAGCAGGATTAGCTGCTGCTGAAGAACTTAATCTACTAGGATATCAAGTAACTATT
  TATGAACGTGCTAGAGAATCAGGCGGTTTATTAATGTATGGTATTCCGAATATGAAACTT
  GATAAAGATGTGGTTCGACGTCGTATTAAGTTAATGGAAGAAGCGGGCATTACTTTCATT
  AATGGTGTTGAAGTCGGCGTTGATATTGATAAAGCAACGTTAGAATCTGAGTATGATGCC
  ATTATATTATGTACTGGTGCACAAAAAGGTAGAGATTTACCTTTAGAAGGACGCATGGGT
  GATGGTATACATTTCGCTATGGATTATTTAACTGAACAAACGCAGTTGTTAAATGGAGAA
  ATTGATGATATAACAATAACTGCAAAAGATAAGAATGTCATTATCATTGGTGCTGGTGAT
  ACAGGGGCAGACTGTGTAGCGACAGCATTAAGAGAAAATTGTAAATCGATTGTTCAATTT
  AATAAATATACGAAATTGCCAGAAGCAATTACATTTACAGAAAATGCATCATGGCCTTTA
  GCAATGCCGGTGTTTAAAATGGACTATGCGCACCAAGAGTACGAAGCTAAGTTTGGTAAG
  GAACCACGTGCATATGGTGTTCAAACAATGCGTTACGATGTTGACGATAAAGGACACATA
  CGTGGTTTGTATACTCAAATTTTAGAGCAAGGCGAAAATGGTATGGTCATGAAAGAAGGA
  CCTGAAAGATTTTGGCCTGCTGACCTTGTATTATTATCAATCGGCTTCGAAGGTACAGAA
  CCAACAGTACCGAATGCTTTTAACATTAAAACGGATAGAAATCGAATCGTGGCGGATGAT
  ACAAACTATCAAACTAATAATGAAAAGGTATTTGCTGCTGGAGATGCTAGACGTGGTCAA
  AGTTTAGTTGTATGGGCAATTAAAGAAGGTAGAGGCGTAGCGAAAGCAGTAGATCAGTAT
  TTAGCTAGTAAAGTTTGTGTATAA
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⊟Protein

⊟General

• locus tag: NWMN_RS02465 [old locus tag: NWMN_0437 ]
• symbol: NWMN_RS02465
• description: glutamate synthase subunit beta
• length: 487
• theoretical pI: 5.04058
• theoretical MW: 54427.6
• GRAVY: -0.386242

⊟Function

• ⊞reaction:
  EC 1.4.1.13?  ExPASy
  Glutamate synthase (NADPH) 2 L-glutamate + NADP⁺ = L-glutamine + 2-oxoglutarate + NADPH
• ⊞TIGRFAM:
  glutamate synthase, NADH/NADPH, small subunit (TIGR01317; EC 1.4.1.-; HMM-score: 612.7)
  and 35 more
  glutamate synthase, small subunit (TIGR01318; HMM-score: 284.6)
  Metabolism Amino acid biosynthesis Glutamate family glutamate synthase (NADPH), homotetrameric (TIGR01316; EC 1.4.1.13; HMM-score: 226.3)
  putative selenate reductase, YgfK subunit (TIGR03315; HMM-score: 161.7)
  mycofactocin system FadH/OYE family oxidoreductase 2 (TIGR03997; EC 1.-.-.-; HMM-score: 58.4)
  dihydrolipoyl dehydrogenase (TIGR01350; EC 1.8.1.4; HMM-score: 46.4)
  Metabolism Energy metabolism Electron transport thioredoxin-disulfide reductase (TIGR01292; EC 1.8.1.9; HMM-score: 43.9)
  Cellular processes Cellular processes Detoxification CoA-disulfide reductase (TIGR03385; EC 1.8.1.14; HMM-score: 37.4)
  Unknown function Enzymes of unknown specificity putative bacillithiol system oxidoreductase, YpdA family (TIGR04018; EC 1.8.-.-; HMM-score: 35.7)
  squalene-associated FAD-dependent desaturase (TIGR03467; HMM-score: 30.2)
  Cellular processes Cellular processes Detoxification mercury(II) reductase (TIGR02053; EC 1.16.1.1; HMM-score: 25.8)
  Unknown function Enzymes of unknown specificity flavoprotein, HI0933 family (TIGR00275; HMM-score: 25.1)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiazole biosynthesis enzyme (TIGR00292; HMM-score: 24.4)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll geranylgeranyl reductase (TIGR02023; EC 1.3.1.-; HMM-score: 23.5)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll geranylgeranyl reductase (TIGR02028; HMM-score: 23.1)
  Metabolism Amino acid biosynthesis Aromatic amino acid family shikimate dehydrogenase (TIGR00507; EC 1.1.1.25; HMM-score: 21.1)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other phytoene desaturase (TIGR02734; EC 1.14.99.-; HMM-score: 20.3)
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain (TIGR03197; HMM-score: 20)
  Metabolism Energy metabolism Amino acids and amines sarcosine oxidase, alpha subunit family (TIGR01372; HMM-score: 19.3)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll geranylgeranyl reductase family (TIGR02032; EC 1.3.1.-; HMM-score: 19.2)
  mycofactocin system FadH/OYE family oxidoreductase 1 (TIGR03996; EC 1.-.-.-; HMM-score: 19.2)
  Metabolism Amino acid biosynthesis Other pyrrolysine biosynthesis protein PylD (TIGR03911; HMM-score: 19)
  thioredoxin and glutathione reductase (TIGR01438; EC 1.6.4.-; HMM-score: 18.2)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other C-3',4' desaturase CrtD (TIGR02733; EC 1.3.99.-; HMM-score: 17.2)
  Metabolism Energy metabolism Electron transport glutathione-disulfide reductase (TIGR01424; EC 1.8.1.7; HMM-score: 17.1)
  Metabolism Energy metabolism Electron transport flavocytochrome c (TIGR01813; HMM-score: 16.8)
  lycopene cyclase family protein (TIGR01790; HMM-score: 15.5)
  9,9'-di-cis-zeta-carotene desaturase (TIGR02732; EC 1.3.5.6; HMM-score: 15)
  Cellular processes Cellular processes Detoxification alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 13.2)
  Cellular processes Cellular processes Adaptations to atypical conditions alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 13.2)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone ubiquinone biosynthesis hydroxylase, UbiH/UbiF/VisC/COQ6 family (TIGR01988; EC 1.14.13.-; HMM-score: 12.7)
  putative alkyl hydroperoxide reductase F subunit (TIGR03143; EC 1.6.4.-; HMM-score: 12.6)
  FAD dependent oxidoreductase TIGR03364 (TIGR03364; HMM-score: 12.1)
  Metabolism Central intermediary metabolism Nitrogen metabolism nitrite reductase [NAD(P)H], large subunit (TIGR02374; EC 1.7.1.4; HMM-score: 12)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine glycine oxidase ThiO (TIGR02352; EC 1.4.3.19; HMM-score: 11.9)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin protoporphyrinogen oxidase (TIGR00562; EC 1.3.3.4; HMM-score: 11.7)
• TheSEED: see NWMN_0437
• ⊞PFAM:
  NADP_Rossmann (CL0063) Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 103.3)
  and 25 more
  4Fe-4S (CL0344) Fer4_20; Dihydroprymidine dehydrogenase domain II, 4Fe-4S cluster (PF14691; HMM-score: 80.3)
  NADP_Rossmann (CL0063) DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 35)
  Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 34.6)
  NAD_binding_8; NAD(P)-binding Rossmann-like domain (PF13450; HMM-score: 33.1)
  Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 29.9)
  FAD_binding_3; FAD binding domain (PF01494; HMM-score: 27.6)
  HI0933_like; HI0933-like protein Rossmann domain (PF03486; HMM-score: 27.5)
  Thi4; Thi4 family (PF01946; HMM-score: 23.8)
  Lys_Orn_oxgnase; L-lysine 6-monooxygenase/L-ornithine 5-monooxygenase (PF13434; HMM-score: 23.4)
  Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 23.2)
  AlaDh_PNT_C; Alanine dehydrogenase/PNT, C-terminal domain (PF01262; HMM-score: 21.8)
  2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 20.3)
  FAD_binding_2; FAD binding domain (PF00890; HMM-score: 19.7)
  NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 18.1)
  FMO-like; Flavin-binding monooxygenase-like (PF00743; HMM-score: 17.9)
  FAD_oxidored; FAD dependent oxidoreductase (PF12831; HMM-score: 17.8)
  GIDA; Glucose inhibited division protein A (PF01134; HMM-score: 16.8)
  NAD_binding_9; FAD-NAD(P)-binding (PF13454; HMM-score: 16.6)
  YjeF_N; YjeF-related protein N-terminus (PF03853; HMM-score: 14)
  KARI_N; Acetohydroxy acid isomeroreductase, NADPH-binding domain (PF07991; HMM-score: 13.3)
  MurF-HprK_N (CL0365) MurD-like_N; Mur ligase MurD-like, N-terminal domain (PF21799; HMM-score: 13.3)
  NADP_Rossmann (CL0063) 3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 12)
  Amino_oxidase; Flavin containing amine oxidoreductase (PF01593; HMM-score: 11.8)
  PDDEXK (CL0236) RE_endonuc; Endonuclease domain (PF19778; HMM-score: 11.8)
  NADP_Rossmann (CL0063) Lycopene_cycl; Lycopene cyclase protein (PF05834; HMM-score: 11.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.5317
  • Cytoplasmic Membrane Score: 0.3884
  • Cell wall & surface Score: 0.0001
  • Extracellular Score: 0.0798
• LocateP:
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.069056
  • TAT(Tat/SPI): 0.00348
  • LIPO(Sec/SPII): 0.003297
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446425728 NCBI
• RefSeq: WP_000503583 NCBI
• UniProt: see NWMN_0437

⊟Protein sequence

• MGEFKGFMKYDKQYLGELSLVDRLKHHKAYQQRFTKEDASIQGARCMDCGTPFCQTGQQYGRETIGCPIGNYIPEWNDLVYHQDFKTAYERLSETNNFPDFTGRVCPAPCESACVMKINRESIAIKGIERTIIDEAFENGWVAPKVPSRRRDEKVAIVGSGPAGLAAAEELNLLGYQVTIYERARESGGLLMYGIPNMKLDKDVVRRRIKLMEEAGITFINGVEVGVDIDKATLESEYDAIILCTGAQKGRDLPLEGRMGDGIHFAMDYLTEQTQLLNGEIDDITITAKDKNVIIIGAGDTGADCVATALRENCKSIVQFNKYTKLPEAITFTENASWPLAMPVFKMDYAHQEYEAKFGKEPRAYGVQTMRYDVDDKGHIRGLYTQILEQGENGMVMKEGPERFWPADLVLLSIGFEGTEPTVPNAFNIKTDRNRIVADDTNYQTNNEKVFAAGDARRGQSLVVWAIKEGRGVAKAVDQYLASKVCV

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CodY, GltC see NWMN_0437

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications