PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS01150 [old locus tag: NWMN_0205 ]
• pan locus tag^?: SAUPAN001157000
• symbol: NWMN_RS01150
• pan gene symbol^?: rbsR
• synonym:
• product: LacI family transcriptional regulator

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS01150 [old locus tag: NWMN_0205 ]
• symbol: NWMN_RS01150
• product: LacI family transcriptional regulator
• replicon: chromosome
• strand: -
• coordinates: 262359..263357
• length: 999
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (262359..263357) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_0205

⊟Phenotype

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⊟DNA sequence

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  ATGAAAAAAGTGTCAATTAAAGATGTTGCTAGAGAAGCTGGTGTATCAGTTACAACTGTG
  TCACATATTTTAAATCATAATGATAGTCGTTTTTCCGCAACAACGATAAAAAACGTACAT
  GCTGTTTCAGAACGTTTAGGCTATGCCCCTAATAAACATGCAAAACAATTGCGCGGCAGT
  AAAATTCAAACTATTGGCGTCATTTTGCCTAGCTTAACAAATCCGTTTTTCTCAGCACTG
  ATGCAAAGTATTCATGACCATAAACCATCTGATGTTGATTTATGCTTTTTAACATCTACA
  GCAACTGATTTGTATGACAATATTAAACATTTAATTGATCGAGGTATTGACGGATTAATT
  ATCGCACAATACATATCATCCCCGGACGCCCTAAATAACTATCTAAAGAAACATCATGTA
  CCTTATGTCGTACTGGATCAAAATGACCATCAAGGCTATACAGATTTTGTTCGGACAAAT
  GAATATCAAGGTGGACAACTTGCAGCACAACATTTAATAGAACTCGGTCACAACCATATG
  ATAATTGTTGCACCATATGACATGATGGCGAATATGTCGACTCGTGTCGCTGGATTTGTC
  GATACTTTGCGCGCGAATCAATTGCCAGAACCACAAATCGTCCATACTGAATTATCTAAG
  CGCGGTGGGCTAACCATTGTTGATGACATCATGGTTCAATCTGCCACTGCAATCTTCGCT
  ATTAACGATGAACTCGCTATTGGCATTTTACGAGGACTAATTGAACATGGCATCAGTATC
  CCGAAAGATATCTCATTAATAGGTTATGACGACATTGATTATGCAGCGTACGTCTCGCCA
  CCTTTAACTACTGTGGCACAACCTATAACTGATATTGGCAAAACATCTTTAACCTTATTA
  CTTCAACGATTACAGCACTTAGATAAATCCATTGATATGATTGAATTACCAACGACTTTA
  AAAATTCGTGCAACAACTGGCTATCATCTTTCAAACTAA
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⊟Protein

⊟General

• locus tag: NWMN_RS01150 [old locus tag: NWMN_0205 ]
• symbol: NWMN_RS01150
• description: LacI family transcriptional regulator
• length: 332
• theoretical pI: 6.76486
• theoretical MW: 36657.7
• GRAVY: -0.0650602

⊟Function

• TIGRFAM:
  Signal transduction Regulatory functions DNA interactions catabolite control protein A (TIGR01481; HMM-score: 172.7)
  and 4 more
  Signal transduction Regulatory functions DNA interactions D-fructose-responsive transcription factor (TIGR02417; HMM-score: 117.9)
  Signal transduction Regulatory functions DNA interactions trehalose operon repressor (TIGR02405; HMM-score: 74.3)
  Signal transduction Regulatory functions DNA interactions transcriptional repressor BetI (TIGR03384; HMM-score: 13.9)
  Genetic information processing Protein synthesis tRNA aminoacylation aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, A subunit (TIGR00132; EC 6.3.5.-; HMM-score: 11)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  Periplas_BP (CL0144) Peripla_BP_3; Periplasmic binding protein-like domain (PF13377; HMM-score: 122.2)
  and 16 more
  Peripla_BP_1; Periplasmic binding proteins and sugar binding domain of LacI family (PF00532; HMM-score: 82.2)
  Peripla_BP_4; Periplasmic binding protein domain (PF13407; HMM-score: 61.8)
  HTH (CL0123) LacI; Bacterial regulatory proteins, lacI family (PF00356; HMM-score: 57.3)
  HTH_Tnp_ISL3; Helix-turn-helix domain of transposase family ISL3 (PF13542; HMM-score: 20)
  HTH_3; Helix-turn-helix (PF01381; HMM-score: 18.8)
  HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 15.3)
  HTH_23; Homeodomain-like domain (PF13384; HMM-score: 14.6)
  HTH_31; Helix-turn-helix domain (PF13560; HMM-score: 14)
  TetR_N; Bacterial regulatory proteins, tetR family (PF00440; HMM-score: 13.5)
  HTH_7; Helix-turn-helix domain of resolvase (PF02796; HMM-score: 13.4)
  HTH_19; Helix-turn-helix domain (PF12844; HMM-score: 13.4)
  CENP-B_N; CENP-B N-terminal DNA-binding domain (PF04218; HMM-score: 13.1)
  IF2_N; Translation initiation factor IF-2, N-terminal region (PF04760; HMM-score: 13)
  HTH_10; HTH DNA binding domain (PF04967; HMM-score: 12.7)
  no clan defined PucR; Purine catabolism regulatory protein-like family (PF07905; HMM-score: 12.6)
  TnsD; Tn7-like transposition protein D (PF15978; HMM-score: 10.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:
• genes regulated by RbsR*, TF important in Ribose utilization: in N315

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.026708
  • TAT(Tat/SPI): 0.005312
  • LIPO(Sec/SPII): 0.001669
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446680534 NCBI
• RefSeq: WP_000757880 NCBI
• UniProt: see NWMN_0205

⊟Protein sequence

• MKKVSIKDVAREAGVSVTTVSHILNHNDSRFSATTIKNVHAVSERLGYAPNKHAKQLRGSKIQTIGVILPSLTNPFFSALMQSIHDHKPSDVDLCFLTSTATDLYDNIKHLIDRGIDGLIIAQYISSPDALNNYLKKHHVPYVVLDQNDHQGYTDFVRTNEYQGGQLAAQHLIELGHNHMIIVAPYDMMANMSTRVAGFVDTLRANQLPEPQIVHTELSKRGGLTIVDDIMVQSATAIFAINDELAIGILRGLIEHGISIPKDISLIGYDDIDYAAYVSPPLTTVAQPITDIGKTSLTLLLQRLQHLDKSIDMIELPTTLKIRATTGYHLSN

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: SigB see NWMN_0205

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications