PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_2568 [new locus tag: NWMN_RS14735 ]
• pan locus tag^?: SAUPAN006414000
• symbol: icaC
• pan gene symbol^?: icaC
• synonym:
• product: intercellular adhesion protein IcaC

⊟Additional information (user-provided)

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_2568 [new locus tag: NWMN_RS14735 ]
• symbol: icaC
• product: intercellular adhesion protein IcaC
• replicon: chromosome
• strand: +
• coordinates: 2835130..2836182
• length: 1053
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332030 NCBI
• RefSeq: YP_001333602 NCBI
• BioCyc:
• MicrobesOnline: 3708170 MicrobesOnline

⊟Phenotype

⊟Additional information (user-provided)

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  
  ATGAAAAAGATTAGACTTGAACTCGTATATTTACGTGCTATTATATGTGCAATTATTATT
  ATCACACATTTACTTACACAAATTACTTTAAAACATGAAAATATGGAGGGTGGATCCTTA
  GTGTTACAATTTTACATTCGTAATATTGTGATTTTTGGTACACCTTGCTTTATTATCTTG
  TCACAGTTACTGACAACCTTGAATTACCAAAAAGTCACCTATAGATACTTAACTACACGC
  GTAAAATATATACTTATTCCTTACATATTAATGGGATTGTTTTACAGTTATAGTGAATCA
  TTATTAACAGATTCAAGTTTCAATAAACAATTCATTGAAAATGTCCTATTAGGTCAATGG
  TATGGCTATTTTATCGTTGTTATCATGCAATTCTTTATTTTGAGTTATATCATTTTTAAA
  ATTAACTATAACCTATTCAACAGTAAAATATTATTATTGTTATCTTTTATTTTACAGCAA
  TCATTTTTATATTACTTTACGAACAACACAGCGTTTCACGATACCGTGCTACACTATTAT
  CCATTAAGTGAAAATACTATAATATTCGGATGGATTTTTTATTTCTTCTTAGGTGCATAT
  ATGGGTTATAACTACGAACGTGTATTAAATTTCTTAGAACGTTATTTAGTTATTATGATT
  GTATTAGCTGTAGCTACTTATTTTGTGTTTATTGCGTTAGCAAATGGAGACTATTGGAAC
  GTTACCAGCTTTTCATATTCATTAACACCATATAATAGTATTATGTTTATTGTTATCTTG
  GGTATTTGCACGCATTTTAAAACAATGTTATTTAATACGATTCAAATGATTAGTGCTTTC
  TCATTCTTTATTTATTTATTACATCCAATCATTCTAGACTCATTGTTTGCATATACAAAT
  ATATTTGAGGATAATACAATGGTCTTTCTAGCGATATCACTACTATTCATTTTAGGATTA
  TGTATAGGTGTCGGCATGATATTGCGTGAATTCTATATCTTTAGGTTTATTATTGGAAAA
  CAACCATATAAATTGAACATTAATGCTTATTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
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  840
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  960
  1020
  1053
  

⊟Protein

⊟General

• locus tag: NWMN_2568 [new locus tag: NWMN_RS14735 ]
• symbol: IcaC
• description: intercellular adhesion protein IcaC
• length: 350
• theoretical pI: 8.82602
• theoretical MW: 41344
• GRAVY: 0.806571

⊟Function

• TIGRFAM:
• TheSEED  :
  • Polysaccharide intercellular adhesin (PIA) biosynthesis protein IcaC
  Regulation and Cell signaling Quorum sensing and biofilm formation Biofilm formation in Staphylococcus  Polysaccharide intercellular adhesin (PIA) biosynthesis protein IcaC
• PFAM:
  Acyl_transf_3 (CL0316) Acyl_transf_3; Acyltransferase domain (PF01757; HMM-score: 106.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 10
• DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.9995
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0005
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.078332
  • TAT(Tat/SPI): 0.000265
  • LIPO(Sec/SPII): 0.011994
• predicted transmembrane helices (TMHMM): 10

⊟Accession numbers

• GI: 151222780 NCBI
• RefSeq: YP_001333602 NCBI
• UniProt: A0A0H3KAR4 UniProt

⊟Additional information (user-provided)

⊟Protein sequence

• MKKIRLELVYLRAIICAIIIITHLLTQITLKHENMEGGSLVLQFYIRNIVIFGTPCFIILSQLLTTLNYQKVTYRYLTTRVKYILIPYILMGLFYSYSESLLTDSSFNKQFIENVLLGQWYGYFIVVIMQFFILSYIIFKINYNLFNSKILLLLSFILQQSFLYYFTNNTAFHDTVLHYYPLSENTIIFGWIFYFFLGAYMGYNYERVLNFLERYLVIMIVLAVATYFVFIALANGDYWNVTSFSYSLTPYNSIMFIVILGICTHFKTMLFNTIQMISAFSFFIYLLHPIILDSLFAYTNIFEDNTMVFLAISLLFILGLCIGVGMILREFYIFRFIIGKQPYKLNINAY

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: icaA > icaD > icaB > icaC

⊟Regulation

• regulators: CodY (repression) regulon, IcaR (repression) regulon

  CodY	(TF)	important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise
  IcaR	(TF)	important in Intercellular adhesion; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Additional information (user-provided)

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Additional information (user-provided)

⊟Other information (user-provided)

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications

Ursula Fluckiger, Martina Ulrich, Andrea Steinhuber, Gerd Döring, Dietrich Mack, Regine Landmann, Christiane Goerke, Christiane Wolz
Biofilm formation, icaADBC transcription, and polysaccharide intercellular adhesin synthesis by staphylococci in a device-related infection model.
Infect Immun: 2005, 73(3);1811-9
[PubMed:15731082] [WorldCat.org] [DOI] (P p)C Heilmann, O Schweitzer, C Gerke, N Vanittanakom, D Mack, F Götz
Molecular basis of intercellular adhesion in the biofilm-forming Staphylococcus epidermidis.
Mol Microbiol: 1996, 20(5);1083-91
[PubMed:8809760] [WorldCat.org] [DOI] (P p)