From AureoWiki
Jump to navigation Jump to search

NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2465 [new locus tag: NWMN_RS14160 ]
  • pan locus tag?: SAUPAN006229000
  • symbol: NWMN_2465
  • pan gene symbol?: crtO
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_2465 [new locus tag: NWMN_RS14160 ]
  • symbol: NWMN_2465
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2713289..2713786
  • length: 498
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGAAAACCATGAAAAAATATATTAAAACAGCATTTTTTTGTAGTATGTATTGGTTAATT
    GTTCAACTAAATATAGCAAATTTAGGTACAAGAATTCCTGATAAGTATTTTCGTCAGAAG
    TACATAATATTTAAATCATTTAACTTTGAGAAGCATGGAAAATTTTGGAACAAATGGTTT
    TACGTAAGAAAATGGAAACATAAGATTTTAGATGGTCATCAGCTTAATCAAAATATATAT
    GATCAGCGTCATTTAATGACAATCAATACTGATGAAATTGAAAAAATGATTATAGAGACA
    AAGAGGGCAGAGTTGATTCATTGGATATCGATACTTCCAGTCATCATATTCAATAAAGGC
    CCTCGTTTAGTAAAGTATATAAATATTTTCTATGCAATGATAGCTAATGTTCCAATCATT
    ATTGTGCAACGCTATAATCGACCGAGATTAACGCAGTTACTACGCATATTAAAACGAAAA
    GGTGAACGTCATGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    498

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_2465 [new locus tag: NWMN_RS14160 ]
  • symbol: NWMN_2465
  • description: hypothetical protein
  • length: 165
  • theoretical pI: 10.8542
  • theoretical MW: 20291.1
  • GRAVY: -0.295758

⊟Function[edit | edit source]

  • reaction:
    EC 2.3.1.-?  ExPASy
  • TIGRFAM:
  • TheSEED  :
    • Glycosyl-4,4'-diaponeurosporenoate acyltransferase precursor (EC 2.3.1.-)
    Fatty Acids, Lipids, and Isoprenoids Isoprenoids Polyprenyl Diphosphate Biosynthesis  Glycosyl-4,4'-diaponeurosporenoate acyltransferase precursor (EC 2.3.1.-)
  • PFAM:
    no clan defined CrtO; Glycosyl-4,4'-diaponeurosporenoate acyltransferase (PF18927; HMM-score: 84.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0015
    • Cytoplasmic Membrane Score: 0.9977
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0008
  • ⊞LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: 4
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.224188
    • TAT(Tat/SPI): 0.002276
    • LIPO(Sec/SPII): 0.056869
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKTMKKYIKTAFFCSMYWLIVQLNIANLGTRIPDKYFRQKYIIFKSFNFEKHGKFWNKWFYVRKWKHKILDGHQLNQNIYDQRHLMTINTDEIEKMIIETKRAELIHWISILPVIIFNKGPRLVKYINIFYAMIANVPIIIVQRYNRPRLTQLLRILKRKGERHD

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • ⊞regulator: SigB (activation) regulon

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)
  2. ↑ Bettina Schulthess, Dominik A Bloes, Patrice François, Myriam Girard, Jacques Schrenzel, Markus Bischoff, Brigitte Berger-Bächi
    The σB-dependent yabJ-spoVG operon is involved in the regulation of extracellular nuclease, lipase, and protease expression in Staphylococcus aureus.
    J Bacteriol: 2011, 193(18);4954-62
    [PubMed:21725011] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=NWMN_2465&oldid=73248"