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NCBI: 06-JUL-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_2462 [new locus tag: NWMN_RS14145 ]
- pan locus tag?: SAUPAN006226000
- symbol: crtM
- pan gene symbol?: crtM
- synonym:
- product: squalene desaturase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_2462 [new locus tag: NWMN_RS14145 ]
- symbol: crtM
- product: squalene desaturase
- replicon: chromosome
- strand: -
- coordinates: 2709770..2710633
- length: 864
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 5331839 NCBI
- RefSeq: YP_001333496 NCBI
- BioCyc:
- MicrobesOnline: 3708064 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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841ATGACAATGATGGATATGAATTTTAAATATTGTCATAAAATCATGAAGAAACATTCAAAA
AGCTTTTCTTACGCTTTTGACTTGTTACCAGAAGATCAAAGAAAAGCGGTTTGGGCAATT
TATGCTGTGTGTCGTAAAATTGATGACAGTATAGATGTTTATGGCGATATTCAATTTTTA
AATCAAATAAAAGAAGATATACAATCTATTGAAAAATACCCATATGAACATCATCACTTT
CAAAGTGATCGTAGAATCATGATGGCGCTTCAGCATGTTGCACAACATAAAAATATCGCC
TTTCAATCTTTTTATAATCTCATTGATACTGTATATAAAGATCAACATTTTACAATGTTT
GAAACGGACGCTGAATTATTCGGATATTGTTATGGTGTTGCTGGTACAGTAGGTGAAGTA
TTGACGCCGATTTTAAGTGATCATGAAACACATCAGACATACGATGTCGCAAGAAGACTT
GGTGAATCGTTGCAATTGATTAATATATTAAGAGATGTCGGTGAAGATTTTGACAATGAA
CGGATATATTTTAGTAAGCAACGATTAAAGCAATATGAAGTTGATATTGCTGAAGTGTAC
CAAAATGGTGTTAATAATCATTATATTGACTTATGGGAATATTATGCAGCTATCGCAGAA
AAAGATTTTCAAGATGTTATGGATCAAATCAAAGTATTTAGTATTGAAGCACAACCAATC
ATAGAATTAGCAGCACGTATATATATTGAAATACTGGACGAAGTGAGACAGGCTAACTAT
ACATTACATGAACGTGTTTTTGTGGATAAGAGGAAAAAGGCAAAGTTGTTTCATGAAATA
AATAGTAAATATCATAGAATATAG60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_2462 [new locus tag: NWMN_RS14145 ]
- symbol: CrtM
- description: squalene desaturase
- length: 287
- theoretical pI: 6.01098
- theoretical MW: 34230.7
- GRAVY: -0.449477
⊟Function[edit | edit source]
- reaction: EC 2.5.1.96? ExPASy4,4'-diapophytoene synthase 2 (2E,6E)-farnesyl diphosphate = 15-cis-4,4'-diapophytoene + 2 diphosphate
- TIGRFAM: squalene synthase HpnD (TIGR03465; EC 2.5.1.21; HMM-score: 144.9)and 2 moresqualene synthase HpnC (TIGR03464; EC 2.4.1.21; HMM-score: 101.2)farnesyl-diphosphate farnesyltransferase (TIGR01559; EC 2.5.1.21; HMM-score: 51.1)
- TheSEED :
- Dehydrosqualene synthase (EC 2.5.1.96)
and 3 more - PFAM: Terp_synthase (CL0613) SQS_PSY; Squalene/phytoene synthase (PF00494; HMM-score: 223.9)and 1 moreTPR (CL0020) ARM_TT21_5th; TT21 fifth ARM repeats domain (PF25064; HMM-score: 12.7)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- DeepLocPro: Cytoplasmic
- Cytoplasmic Score: 0.9777
- Cytoplasmic Membrane Score: 0.0192
- Cell wall & surface Score: 0.0002
- Extracellular Score: 0.0029
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.003464
- TAT(Tat/SPI): 0.000176
- LIPO(Sec/SPII): 0.000456
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MTMMDMNFKYCHKIMKKHSKSFSYAFDLLPEDQRKAVWAIYAVCRKIDDSIDVYGDIQFLNQIKEDIQSIEKYPYEHHHFQSDRRIMMALQHVAQHKNIAFQSFYNLIDTVYKDQHFTMFETDAELFGYCYGVAGTVGEVLTPILSDHETHQTYDVARRLGESLQLINILRDVGEDFDNERIYFSKQRLKQYEVDIAEVYQNGVNNHYIDLWEYYAAIAEKDFQDVMDQIKVFSIEAQPIIELAARIYIEILDEVRQANYTLHERVFVDKRKKAKLFHEINSKYHRI
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: SigB (activation) regulon
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You can add further information about the gene and protein here. [edit]
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
J Bacteriol: 2004, 186(13);4085-99
[PubMed:15205410] [WorldCat.org] [DOI] (P p) - ↑ Bettina Schulthess, Dominik A Bloes, Patrice François, Myriam Girard, Jacques Schrenzel, Markus Bischoff, Brigitte Berger-Bächi
The σB-dependent yabJ-spoVG operon is involved in the regulation of extracellular nuclease, lipase, and protease expression in Staphylococcus aureus.
J Bacteriol: 2011, 193(18);4954-62
[PubMed:21725011] [WorldCat.org] [DOI] (I p)