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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2198 [new locus tag: NWMN_RS12685 ]
  • pan locus tag?: SAUPAN005764000
  • symbol: NWMN_2198
  • pan gene symbol?: —
  • synonym:
  • product: transcriptional regulator AraC family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_2198 [new locus tag: NWMN_RS12685 ]
  • symbol: NWMN_2198
  • product: transcriptional regulator AraC family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2417148..2419103
  • length: 1956
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGACAGATAATTATTCACTACATATTTATAAAAGTATGAAAATGAGTAGTGTGAAGGAA
    GCGAACTTAAAAATTATATATTGGCTACAAGGTACAGGTGTAGTTTCAATCAATCTACAA
    CAATATGATGTAAAAAGAAATGATGTCACCATTATTATGTTGAATGATTTGTATCAAATT
    GATGGTAATGATGATAGTGTATGTTGTGTTGTTGAAGTAAGTGCAAAGACATTTTTGAAG
    TTTATGAATGCAAACTATATGATGCGTGGCAAGATATTAACAAATGAGGTTGCTAGCACT
    TTTAGAATCTTGATTAAGTATTTAATCTACAGCAAAATTAAACAACAGTCTTATAATGCT
    AATGATAAGATTATTAATTACATGTGTGTTGAATTAATGTCATTAAATACATACGATGGT
    AGTCGTCATTTAGTAGCCGAAGAAGTGCACGATTATTTAACAAACAACCACCATAAAAAG
    ATAAATAGGAAAGATGTCATTAATCAAGTTAGTATTACAAACAAAGCTTTAAGTGACATG
    TTTAAAGCGACACCTTATAGTAATTTTATTCAATATTTAAATCATATTAGACTGGAGCAT
    TGCTTAATTGATATTTTAACTTCTAAGAAGCCTATTGAAGAAATCGCAAGTAGTCATGGT
    TTTAATCATTATTCTCGTTTTATACACCTCTTTAAAGAAACGTATGGTAACACTCCTAAA
    TTAATTAGAAAAAGATATAGTCCAACATCTCATTCAATTAACCATTCCCAATTAGTTGAA
    ATTGATAAAAATATAATTGAATTATTTGATGATACTAATCAAAGTTCTTCAAGTATGCGC
    GAGATTGATATACCTTTTGAGCCTACAAAAAGGAGTCAAATGTATCAACCTAAAAACATT
    TATATTAAAGGAAGTAATTTTTATTGGATGGATTATTATACTATTAAACAAATCATTCAA
    AGATTAGGTGTTAATCCTGAAAACTTGCATATAATTGTTACAATCGACACAAGCAATGAG
    CTATCGATTGAACAAATCACTTTATTACTTCAAAAGATAGTGAATTACAATGTAAATGTA
    GTGTTTCGTATTAAACATGAATATAAGGAATTGTTAACGAGTGCTGAAAGAGGCAAGATA
    GAAAAGCTAGTAGCTACAATTTTTAATATGACTTTTGAGAGTAATAGATGTAAGATTGCA
    TTTTTAATTAACGACTTAAATACTACTGCAATTCAAAAACTGAAACGTTTAATAGATAAT
    TATATAGGAGTATTTGAATTAATATATCATCTGGAACCAAAAGAGTTGAATACGCGCTAT
    GATAAAGAAATTGATCATCTAATAGACTATTTTATCTTACCGCTAAGACAAATAGAACAG
    GTGTCTATTGCACGTTCTAAAATTATATGTGATGTAGATTTGATTAATGAGAATAATGAC
    TTAAAAGAAAAAATCACAATGTCTGATATTAATAAGGTTTCTGAACTTTTGACAAATTAT
    ACAAAATTTCGAGGACTTATTATGCACATGTCAATTATAAGTGAATCGAGCAAAGAAATA
    AATTTAACTAGATTATCAATATTCATTTTTATCATAAATATACTTAATCAATTACGAGGG
    ATTGTTATTTATCAAAATGACACGATGATTGTAACGAAGTTCAAACATGAAGTACAATGT
    GTCATTTGTTTGCCAGCCGCATTGTTAAATGAGAATTCAGACGCTATACGTTTATCGTGT
    AAGGGTCTTCAACAATTTAGCCATGCTAAAACTAAACAAATTGTTTTAAGTATAGATAAA
    GATAATCATACTTCCGAAAATGGTGTACTTGTGAATGATATTATGCAACAACACAGTTAT
    TGGAAAGCTAATTTTATAGAAAGAAATATTTATGATCATTCATTAATGGTCTCAAGCAAT
    AGTATTGTTCATTTGAAATATCATCTTGAGATATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1956

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_2198 [new locus tag: NWMN_RS12685 ]
  • symbol: NWMN_2198
  • description: transcriptional regulator AraC family protein
  • length: 651
  • theoretical pI: 8.28527
  • theoretical MW: 76005.3
  • GRAVY: -0.224578

⊟Function[edit | edit source]

  • TIGRFAM:
    YSIRK-targeted surface antigen transcriptional regulator (TIGR04094; HMM-score: 38.1)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    HTH (CL0123) HTH_18; Helix-turn-helix domain (PF12833; HMM-score: 54.6)
    and 1 more
    HTH_AraC; Bacterial regulatory helix-turn-helix proteins, AraC family (PF00165; HMM-score: 30.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.019049
    • TAT(Tat/SPI): 0.00021
    • LIPO(Sec/SPII): 0.000901
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTDNYSLHIYKSMKMSSVKEANLKIIYWLQGTGVVSINLQQYDVKRNDVTIIMLNDLYQIDGNDDSVCCVVEVSAKTFLKFMNANYMMRGKILTNEVASTFRILIKYLIYSKIKQQSYNANDKIINYMCVELMSLNTYDGSRHLVAEEVHDYLTNNHHKKINRKDVINQVSITNKALSDMFKATPYSNFIQYLNHIRLEHCLIDILTSKKPIEEIASSHGFNHYSRFIHLFKETYGNTPKLIRKRYSPTSHSINHSQLVEIDKNIIELFDDTNQSSSSMREIDIPFEPTKRSQMYQPKNIYIKGSNFYWMDYYTIKQIIQRLGVNPENLHIIVTIDTSNELSIEQITLLLQKIVNYNVNVVFRIKHEYKELLTSAERGKIEKLVATIFNMTFESNRCKIAFLINDLNTTAIQKLKRLIDNYIGVFELIYHLEPKELNTRYDKEIDHLIDYFILPLRQIEQVSIARSKIICDVDLINENNDLKEKITMSDINKVSELLTNYTKFRGLIMHMSIISESSKEINLTRLSIFIFIINILNQLRGIVIYQNDTMIVTKFKHEVQCVICLPAALLNENSDAIRLSCKGLQQFSHAKTKQIVLSIDKDNHTSENGVLVNDIMQQHSYWKANFIERNIYDHSLMVSSNSIVHLKYHLEI

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigB (activation) regulon
    SigB(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  [1]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Bettina Schulthess, Dominik A Bloes, Patrice François, Myriam Girard, Jacques Schrenzel, Markus Bischoff, Brigitte Berger-Bächi
    The σB-dependent yabJ-spoVG operon is involved in the regulation of extracellular nuclease, lipase, and protease expression in Staphylococcus aureus.
    J Bacteriol: 2011, 193(18);4954-62
    [PubMed:21725011] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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