PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_2132 [new locus tag: NWMN_RS12325 ]
• pan locus tag^?: SAUPAN005682000
• symbol: secY
• pan gene symbol^?: secY
• synonym:
• product: preprotein translocase subunit SecY

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_2132 [new locus tag: NWMN_RS12325 ]
• symbol: secY
• product: preprotein translocase subunit SecY
• replicon: chromosome
• strand: -
• coordinates: 2365378..2366670
• length: 1293
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331718 NCBI
• RefSeq: YP_001333166 NCBI
• BioCyc:
• MicrobesOnline: 3707734 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGATTCAAACCCTTGTGAACTTCTTTAGAACAAAAGAGGTTCGTAACAAGATTTTCTTC
  ACACTAGCAATGTTAGTAATTTTTAAAATAGGGACTTATATACCAGCTCCAGGAGTAAAT
  CCTGCAGCTTTTGATAATCCCCAAGGTTCTCAAGGTGCCACTGAGTTATTAAATACTTTT
  GGTGGCGGAGCCTTGAAACGATTTTCTATTTTTGCAATGGGTATTGTACCCTACATCACT
  GCATCAATCGTAATGCAATTATTACAAATGGATATTGTCCCTAAATTCTCAGAATGGGCA
  AAACAAGGTGAAGTAGGTAGAAGAAAGTTAAATAACGTTACTCGTTATTTAGCAATTTCT
  TTAGCATTTATCCAATCTATAGGTATGGCATTCCAATTTAATAATTATCTCAAAGGTGCG
  CTGATTATCAATCAGTCAATTATGAGTTATTTATTAATAGCACTAGTTTTGACAGCAGGA
  ACTGCTTTCTTAATATGGCTTGGTGATCAAATCACTCAGTTCGGTGTTGGTAATGGTATT
  TCTATTATCATATTTGCGGGTATTTTATCAACGTTGCCAGCCAGTCTAATTCAATTTGGA
  CAAACTGCGTTCGTTGGGCAAGAAGATACATCATTAGCATGGTTGAAAGTATTAGGACTT
  TTAGTGTCACTGATACTATTAACTGTAGGTGCTATTTATGTACTTGAAGCCGTTCGTAAA
  ATTCCGATTCAATATGCTAAGAAACAAACTGCTCAAAGATTAGGTTCACAGGCAACTTAT
  CTACCTTTAAAAGTGAACTCAGCCGGTGTTATTCCTGTAATCTTTGCAATGGCGTTCTTC
  TTACTGCCTAGAACATTAACGTTGTTCTATCCAGATAAAGAATGGGCACAAAACATTGCG
  AATGCTGCAAATCCTTCAAGTAATGTTGGTATGGTTGTATATATTGTACTAATTATACTA
  TTCACATACTTCTATGCATTTGTACAAGTTAATCCGGAAAAAATGGCTGATAACCTTAAG
  AAACAAGGTAGCTATGTTCCAGGTATTAGACCTGGTGAACAAACCAAAAAATATATTACT
  AAAGTTTTATATCGTTTAACTTTTGTAGGTTCTATCTTCTTAGCCGTTATTTCAATTCTA
  CCGATATTAGCAACTAAGTTTATGGGATTACCACAATCAATTCAGATTGGTGGTACAAGT
  TTACTTATCGTTATTGGTGTAGCGATTGAAACAATGAAATCTTTAGAAGCTCAAGTATCT
  CAAAAAGAATATAAAGGCTTTGGTGGTAGATAA
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⊟Protein

⊟General

• locus tag: NWMN_2132 [new locus tag: NWMN_RS12325 ]
• symbol: SecY
• description: preprotein translocase subunit SecY
• length: 430
• theoretical pI: 10.3709
• theoretical MW: 47147.6
• GRAVY: 0.554419

⊟Function

• TIGRFAM:
  Genetic information processing Protein fate Protein and peptide secretion and trafficking preprotein translocase, SecY subunit (TIGR00967; HMM-score: 482.9)
  and 1 more
  Genetic information processing Protein fate Protein and peptide secretion and trafficking accessory Sec system translocase SecY2 (TIGR02920; HMM-score: 209.7)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  no clan defined SecY; SecY translocase (PF00344; HMM-score: 360.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 9
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.461739
  • TAT(Tat/SPI): 0.011703
  • LIPO(Sec/SPII): 0.015722
• predicted transmembrane helices (TMHMM): 10

⊟Accession numbers

• GI: 151222344 NCBI
• RefSeq: YP_001333166 NCBI
• UniProt: A0A0H3K9X7 UniProt

⊟Protein sequence

• MIQTLVNFFRTKEVRNKIFFTLAMLVIFKIGTYIPAPGVNPAAFDNPQGSQGATELLNTFGGGALKRFSIFAMGIVPYITASIVMQLLQMDIVPKFSEWAKQGEVGRRKLNNVTRYLAISLAFIQSIGMAFQFNNYLKGALIINQSIMSYLLIALVLTAGTAFLIWLGDQITQFGVGNGISIIIFAGILSTLPASLIQFGQTAFVGQEDTSLAWLKVLGLLVSLILLTVGAIYVLEAVRKIPIQYAKKQTAQRLGSQATYLPLKVNSAGVIPVIFAMAFFLLPRTLTLFYPDKEWAQNIANAANPSSNVGMVVYIVLIILFTYFYAFVQVNPEKMADNLKKQGSYVPGIRPGEQTKKYITKVLYRLTFVGSIFLAVISILPILATKFMGLPQSIQIGGTSLLIVIGVAIETMKSLEAQVSQKEYKGFGGR

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: adk < secY < rplO < rpmD < rpsE < rplR < rplF < rpsH < rpsN < rplE < rplX < rplN < rpsQ < rpmC < rplP < rpsC < rplV < rpsS < rplB < rplW < rplD < rplC < rpsJ

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications