PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_2033 [new locus tag: NWMN_RS11740 ]
• pan locus tag^?: SAUPAN005424000
• symbol: NWMN_2033
• pan gene symbol^?: —
• synonym:
• product: acetyltransferase, GNAT family protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_2033 [new locus tag: NWMN_RS11740 ]
• symbol: NWMN_2033
• product: acetyltransferase, GNAT family protein
• replicon: chromosome
• strand: -
• coordinates: 2250306..2251166
• length: 861
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332403 NCBI
• RefSeq: YP_001333067 NCBI
• BioCyc:
• MicrobesOnline: 3707626 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGGCTAAAGAACTTTGTTTTGAAGGTATCACTTTAAAAGCATTTGATGAACAATATCGT
  TCAGCAATTAATGATTTTGACTTGAATGAAAGACAACAAATATATTCATCTTTACCTAAA
  GAAGTTATTGATGATGCAATTAATGATGCTGATAGGATTGCTAACGTAGCAATAAACGAT
  AAAAATGAAGTGGTGGGCTTTTTTGTATTACATCGTTACTATCAGCATGAAGGTTATGAT
  ACACCTGAAAATGTCGTTTATATTCGTTCATTATCGATTAATGAAAAATATCAAGGTTTT
  GGATATGGCACGAAAATAATGATGTCATTGCCGCAATATGTTCAAGGTGTATTTCCTGAT
  TTTAATCATCTATATCTAGTAGTAGATGCGGAAAATGACAATGCTTGGAACCTATACGAA
  CGTGCTGGATTCATGCATACAGCGACTAAAGAAGAAGGACCAATTGGCAAAGAACGACTA
  TATTACTTGGACTTAGATTCAAAACATGTTTCATCATTAAAGCTTGAAGAAGAAAGTCGT
  TCAGAAGTGACCAATGTACATATCATTAATTTAATGATTGATGGCCAAAAGGTTGGCTTT
  ATCGCATTGGAGCAGATTGGTGAACGCATGAACATTGCTGCTATTGAAGTGGATAAATCA
  TATCGCTTTAATGGTATTGGTTCAAGTGCTCTGCGACAATTGCCAACTTACTTAAGAAAA
  AACTATGACAACCTTAATGTGATTACGATGATTCTGTTTGGAGAGAATAATGATTTTAAA
  CCATTATGTTTAAATAGTAATTTCGTTGAAATCGAACAAACTGATGATTATGTCGTTTTC
  GAAAAATATTTAAATTACTAA
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⊟Protein

⊟General

• locus tag: NWMN_2033 [new locus tag: NWMN_RS11740 ]
• symbol: NWMN_2033
• description: acetyltransferase, GNAT family protein
• length: 286
• theoretical pI: 4.45913
• theoretical MW: 33110
• GRAVY: -0.377273

⊟Function

• TIGRFAM:
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal-protein-alanine acetyltransferase (TIGR01575; EC 2.3.1.128; HMM-score: 58.5)
  and 2 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs mycothiol synthase (TIGR03448; EC 2.3.1.189; HMM-score: 26.4)
  UDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-altrosamine N-acetyltransferase (TIGR03585; EC 2.3.1.202; HMM-score: 18.8)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  Acetyltrans (CL0257) Acetyltransf_1; Acetyltransferase (GNAT) family (PF00583; HMM-score: 72.9)
  and 10 more
  Acetyltransf_10; Acetyltransferase (GNAT) domain (PF13673; HMM-score: 46.5)
  Acetyltransf_7; Acetyltransferase (GNAT) domain (PF13508; HMM-score: 46.1)
  Acetyltransf_3; Acetyltransferase (GNAT) domain (PF13302; HMM-score: 29.5)
  Acetyltransf_15; Putative acetyl-transferase (PF17013; HMM-score: 25.1)
  FR47; FR47-like protein (PF08445; HMM-score: 24.5)
  Acetyltransf_CG; GCN5-related N-acetyl-transferase (PF14542; HMM-score: 22)
  GNAT_acetyltr_2; GNAT acetyltransferase 2 (PF13718; HMM-score: 18)
  GAF (CL0161) GAF_3; GAF domain (PF13492; HMM-score: 15.4)
  no clan defined Phage_RpbA; Phage RNA polymerase binding, RpbA (PF10789; HMM-score: 13.6)
  Acetyltrans (CL0257) Acetyltransf_8; Acetyltransferase (GNAT) domain (PF13523; HMM-score: 12.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002243
  • TAT(Tat/SPI): 0.000179
  • LIPO(Sec/SPII): 0.000367
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222245 NCBI
• RefSeq: YP_001333067 NCBI
• UniProt: A0A0H3K9N4 UniProt

⊟Protein sequence

• MAKELCFEGITLKAFDEQYRSAINDFDLNERQQIYSSLPKEVIDDAINDADRIANVAINDKNEVVGFFVLHRYYQHEGYDTPENVVYIRSLSINEKYQGFGYGTKIMMSLPQYVQGVFPDFNHLYLVVDAENDNAWNLYERAGFMHTATKEEGPIGKERLYYLDLDSKHVSSLKLEEESRSEVTNVHIINLMIDGQKVGFIALEQIGERMNIAAIEVDKSYRFNGIGSSALRQLPTYLRKNYDNLNVITMILFGENNDFKPLCLNSNFVEIEQTDDYVVFEKYLNY

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications