PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1841 [new locus tag: NWMN_RS10580 ]
• pan locus tag^?: SAUPAN004932000
• symbol: camS
• pan gene symbol^?: camS
• synonym:
• product: CamS sex pheromone cAM373 precursor

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1841 [new locus tag: NWMN_RS10580 ]
• symbol: camS
• product: CamS sex pheromone cAM373 precursor
• replicon: chromosome
• strand: -
• coordinates: 2049804..2051003
• length: 1200
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332314 NCBI
• RefSeq: YP_001332875 NCBI
• BioCyc:
• MicrobesOnline: 3707429 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGAAGCGTACATTAGTATTATTGATTACAGCTATCTTTATACTCGCTGCTTGTGGTAAC
  CATAAGGATGACCAGGCTGGAAAAGATAATCAAAAACATAACAATAGTTCAAATCAAGTA
  AAAGAAATTGCAACGGATAAAAATGTACAAGGTGATAACTATCGTACATTGTTACCATTT
  AAAGAAAGCCAGGCAAGAGGACTTTTACAAGATAACATGGCAAATAGTTATAATGGCGGC
  GACTTTGAAGATGGTTTATTGAACTTAAGTAAAGAAGTATTTCCAACAGATAAATATTTG
  TATCAAGATGGTCAATTTTTGGACAAGAAAACAATTAATGCCTATTTAAATCCTAAGTAT
  ACAAAACGTGAAATCGATAAAATGTCTGAAAAAGATAAAAAAGACAAGAAAGCGAATGAA
  AATTTAGGACTTAATCCATCACACGAAGGTGAAACAGATCCTGAAAAGATTGCAGAAAAA
  TCACCAGCCTATTTATCTAACATTTTAGAGCAAGATTTTTATGGTGGTGGAGATACAAAA
  GGTAAGAATATTAAAGGTATGACGATTGGTTTAGCTATGAATAGTGTTTATTACTATAAA
  AAAGAAAAAGATGGATCGACTTTTAGTAAAAAACTAGATGATAGCGAAGTTAAAAAGCAA
  GGTAAACAAATGGCTAGTGAGATATTATCAAGGTTACGTGAAAATGATGATTTAAAAGAT
  ATACCAATTCATTTTGCAATTTATAAGCAATCAAGTGAAGATTCAATCACACCAGGTGAA
  TTTATCACTCAAGCGACTGCAGAAAAGAGTCAAACAAAGCTTAATGAATGGCATAATATC
  AATGAAAAATCAGCTTTATTACCTTCTTCAACAGCAGCAGATTATGATGAAAATTTAAAT
  AATAATTTCAAGCAATTTAATGATAATTTGCAATCATATTTTTCTAATTTCACACAAGCA
  GTAGGGAAAGTTAAATTTGTTGATAAAAAGCCACAACGATTAGTAGTAGATTTACCAATC
  GATTACTATGGACAAGCTGAAACAATTGGTATTACACAGTACGTTACTGAACAAGCGAAT
  AAATATTTCGATAAAATCGATAACTATGAAATTCGTATTAAAGATGGTAACCAACCACGT
  GCTTTAATTAGTAAGACAAAAGATGACAAAGAACCGCAAGTTCATATTTACAGTAATTAA
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⊟Protein

⊟General

• locus tag: NWMN_1841 [new locus tag: NWMN_RS10580 ]
• symbol: CamS
• description: CamS sex pheromone cAM373 precursor
• length: 399
• theoretical pI: 6.29166
• theoretical MW: 45380.3
• GRAVY: -0.927569

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Sporulation and germination sporulation lipoprotein, YhcN/YlaJ family (TIGR02898; HMM-score: 12.9)
• TheSEED  :
  • Putative pheromone cAM373 precursor lipoprotein CamS
  Regulation and Cell signaling Regulation and Cell signaling - no subcategory Sex pheromones in Enterococcus faecalis and other Firmicutes  Putative pheromone cAM373 precursor lipoprotein CamS
• PFAM:
  CamS-like (CL0878) CamS; CamS sex pheromone cAM373 precursor (PF07537; HMM-score: 372.9)
  and 4 more
  no clan defined DUF6491; Family of unknown function (DUF6491) (PF20101; HMM-score: 14.5)
  AAR2_1st; AAR2 N-terminal domain (PF20981; HMM-score: 13.7)
  IBIN; Induced by infection (PF22859; HMM-score: 11.9)
  LppaM (CL0421) LPAM_1; Prokaryotic membrane lipoprotein lipid attachment site (PF08139; HMM-score: 8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 3.33
  • Cellwall Score: 3.33
  • Extracellular Score: 3.33
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.0004
  • Cytoplasmic Membrane Score: 0.8944
  • Cell wall & surface Score: 0.018
  • Extracellular Score: 0.0872
• LocateP: Lipid anchored
  • Prediction by SwissProt Classification: Extracellular
  • Pathway Prediction: Sec-(SPII)
  • Intracellular possibility: 0
  • Signal peptide possibility: 0.5
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: ILAACGN
• SignalP: Signal peptide LIPO(Sec/SPII) length 17 aa
  • SP(Sec/SPI): 0.000545
  • TAT(Tat/SPI): 0.000098
  • LIPO(Sec/SPII): 0.999003
  • Cleavage Site: CS pos: 17-18. LAA-CG. Pr: 0.9998
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222053 NCBI
• RefSeq: YP_001332875 NCBI
• UniProt: A0A0H3KHJ9 UniProt

⊟Protein sequence

• MKRTLVLLITAIFILAACGNHKDDQAGKDNQKHNNSSNQVKEIATDKNVQGDNYRTLLPFKESQARGLLQDNMANSYNGGDFEDGLLNLSKEVFPTDKYLYQDGQFLDKKTINAYLNPKYTKREIDKMSEKDKKDKKANENLGLNPSHEGETDPEKIAEKSPAYLSNILEQDFYGGGDTKGKNIKGMTIGLAMNSVYYYKKEKDGSTFSKKLDDSEVKKQGKQMASEILSRLRENDDLKDIPIHFAIYKQSSEDSITPGEFITQATAEKSQTKLNEWHNINEKSALLPSSTAADYDENLNNNFKQFNDNLQSYFSNFTQAVGKVKFVDKKPQRLVVDLPIDYYGQAETIGITQYVTEQANKYFDKIDNYEIRIKDGNQPRALISKTKDDKEPQVHIYSN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: camS < lig < pcrA < pcrB

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications