PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1814 [new locus tag: NWMN_RS10425 ]
• pan locus tag^?: SAUPAN004876000
• symbol: int
• pan gene symbol^?: int2
• synonym:
• product: phage integrase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1814 [new locus tag: NWMN_RS10425 ]
• symbol: int
• product: phage integrase
• replicon: chromosome
• strand: +
• coordinates: 2022127..2023173
• length: 1047
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331101 NCBI
• RefSeq: YP_001332848 NCBI
• BioCyc:
• MicrobesOnline: 3707402 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  1021
  
  ATGCCAGTATATAAGGATGATAATACAGGTAAATGGTATTTTTCCATTAGATATAAAGAT
  GTATACGGTAATAACAAACGAAAAATGAAGCGTGGGTTTGAACGTAAGAAAGATGCCAAA
  CTAGCTGAAAGCGAATTTATACAAAATGTTAAATATGGATACTCGGACAATCAACCCTTT
  GAATATATATTTTTTAATCGTTTAAAAAATGAAAATCTTTCTGCACGCTCAATAGAAAAG
  CGAACTACAGAATATAATACTCACATAAAAGAAAGGTTCGGAAATATCCCTATTGGCAAA
  ATCACTACTACGCAATGTACTGCTTTCAGGAATTATTTGTTAAACGATGCAGGTCTTTCT
  GTTGGCTATGCACGATCTGTGTGGGCAGGTTTTAAAGCAGTTATCAATTACGCCAAAAAG
  CATTACAAGCTCTTATACGACCCCACATTATCGGTAACTCCTATTCCCAGAACAAAACCA
  CAAGCTAAATTTATCACTCGTGAAGAATTTGATGAAAAAGTAGAACAAATCACAAACGAT
  ACTTCTCGTCAGCTAACTAAACTGTTATTTTATTCTGGTCTTAGAATAGGCGAAGCTTTA
  GCTTTGCAGTGGAAAGATTACGATAAAATAAAAGGCGAAATTGACGTAAATAAGAAAATC
  AATTTAAGTAATAGAGAAATTGAATATAATCTAAAAAAAGAAAGTTCTAAAGGGATAATA
  CCTGTACCAAAATTAATTAGAGAGATGCTTAAAAACATGTATAATGAATCTTCTAAAAGA
  TATAAATATTTTGACGAAAACTATTTTATATTCGGGGGGTTAGAACCTATTAGATACGTT
  ACCTATTCGTATCATTTTAAATCTGTATTCCCGAATCTAAAAATACACCATTTAAGACAC
  TCGTACGCAAGCTATTTAATTAATAATGGTGTAGATATGTATTTATTAATGGAATTAATG
  AGGCACTCTAACATTACAGAAACAATTCAAACGTACTCTCATTTATATACTGATAAAAAA
  CATCAAGCTATGAACATATTTGATTAA
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⊟Protein

⊟General

• locus tag: NWMN_1814 [new locus tag: NWMN_RS10425 ]
• symbol: Int
• description: phage integrase
• length: 348
• theoretical pI: 9.97772
• theoretical MW: 41333
• GRAVY: -0.712356

⊟Function

• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerC (TIGR02224; HMM-score: 53)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerD (TIGR02225; HMM-score: 46.6)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair integron integrase (TIGR02249; HMM-score: 43.6)
  Genetic information processing Mobile and extrachromosomal element functions Other integron integrase (TIGR02249; HMM-score: 43.6)
• TheSEED: data available for NCTC8325
• PFAM:
  DNA-mend (CL0382) Phage_integrase; Phage integrase family (PF00589; HMM-score: 70.4)
  and 4 more
  MBD-like (CL0081) Arm-DNA-bind_4; Arm DNA-binding domain (PF14657; HMM-score: 45.8)
  l-integrase_N (CL0469) Phage_int_SAM_3; Phage integrase, N-terminal SAM-like domain (PF14659; HMM-score: 30.6)
  Phage_int_SAM_5; Phage integrase SAM-like domain (PF13102; HMM-score: 26.6)
  no clan defined DUF5126; Domain of unknown function (DUF5126) (PF17166; HMM-score: 9.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.014878
  • TAT(Tat/SPI): 0.00072
  • LIPO(Sec/SPII): 0.004127
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222026 NCBI
• RefSeq: YP_001332848 NCBI
• UniProt: A0A0H3K9E8 UniProt

⊟Protein sequence

• MPVYKDDNTGKWYFSIRYKDVYGNNKRKMKRGFERKKDAKLAESEFIQNVKYGYSDNQPFEYIFFNRLKNENLSARSIEKRTTEYNTHIKERFGNIPIGKITTTQCTAFRNYLLNDAGLSVGYARSVWAGFKAVINYAKKHYKLLYDPTLSVTPIPRTKPQAKFITREEFDEKVEQITNDTSRQLTKLLFYSGLRIGEALALQWKDYDKIKGEIDVNKKINLSNREIEYNLKKESSKGIIPVPKLIREMLKNMYNESSKRYKYFDENYFIFGGLEPIRYVTYSYHFKSVFPNLKIHHLRHSYASYLINNGVDMYLLMELMRHSNITETIQTYSHLYTDKKHQAMNIFD

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: int > NWMN_1815

⊟Regulation

• data available for NCTC8325

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications